Qualitative and quantitative proteomic profiling of cripto-1- embryonic stem cells by means of accurate mass LC-MS analysis

Cripto is one of the key regulators of embryonic stem cells (ESCs) differentiation into cardiomyocites vs neuronal fate. Cripto-/- murine ESCs have been utilized to investigate the molecular mechanisms underlying early events of mammalian lineage differentiation. 2D/LC-MS/MS and a label-free LC-MS approaches were used to qualitatively and quantitatively profile the cripto-/- ESC proteome, providing an integral view of the alterations induced in stem cell functions by deleting the cripto gene.Cripto is one of the key regulators of embryonic stem cells (ESCs) differentiation into cardiomyocites vs neuronal fate. Cripto-/- murine ESCs have been utilized to investigate the molecular mechanisms underlying early events of mammalian lineage differentiation. 2D/LC-MS/MS and a label-free LC-MS approaches were used to qualitatively and quantitatively profile the cripto-/- ESC proteome, providing an integral view of the alterations induced in stem cell functions by deleting the cripto gene. © 2009 American Chemical Society