1,089 research outputs found
Development of high sensitive real-time PCR to detect mustard and other allergens of the family Brassicaceae in food samples
Mustard is a commonly used condiment including in production of other food products. As mustard is an allergen, it is necessary to control its presence. The development of PCR test-systems for its detection is complicated by the fact that this condiment can be made from seeds of various plant species (Brassica juncea, Brassica nigra, Sinapis alba) of the family Brassicaceae that are not closely related. This family includes other plant species such as white cabbage (Brassica oleracea) and rapeseed (Brassica napus), which can cause the allergic reaction, although seldom. In this connection, many authors use primers specific to many species of this family, including to allergens, to detect mustard. In this work, we used the similar strategy. To increase sensitivity, primers for the mitochondrial COX gene were selected. To increase PCR stability in analysis of deeply processed products, primers were selected for a region with a length of 61 base pair. In the work, the specificity and sensitivity of the developed PCR method was confirmed. Analyses of different products, including those that underwent deep technological processing, were carried out with these primers. Also, primers were selected to detect white mustard (S. alba). When analyzing products on the presence of white mustard, charac‑ teristic regional preferences were demonstrated: this species is used in manufacturing products mainly in the UK and USA
Synthesis of Carboxymethyl Starch for increasing drilling mud quality in drilling oil and gas wells
This paper describes the impact of carboxymethyl starch preparation conditions on physicochemical properties of polysaccharide reagent, widely used as fluid loss reducing agent in drilling mud. Variation of the main parameters of carboxymethylation is researched in the experiment. The following conditions such as temperature and reaction time, amount of water, as well as ratio of NaOH to monochloracetic acid define the characteristics of carboxymethyl starch. The degree of substitution is defined for polysaccharides, as well as the characteristics of samples have been studied by infrared spectroscopy. Rheological characteristics and fluid loss indicator have been investigated to study the impact of the reagents on drilling mud quality
Flame front propagation IV: Random Noise and Pole-Dynamics in Unstable Front Propagation II
The current paper is a corrected version of our previous paper
arXiv:adap-org/9608001. Similarly to previous version we investigate the
problem of flame propagation. This problem is studied as an example of unstable
fronts that wrinkle on many scales. The analytic tool of pole expansion in the
complex plane is employed to address the interaction of the unstable growth
process with random initial conditions and perturbations. We argue that the
effect of random noise is immense and that it can never be neglected in
sufficiently large systems. We present simulations that lead to scaling laws
for the velocity and acceleration of the front as a function of the system size
and the level of noise, and analytic arguments that explain these results in
terms of the noisy pole dynamics.This version corrects some very critical
errors made in arXiv:adap-org/9608001 and makes more detailed description of
excess number of poles in system, number of poles that appear in the system in
unit of time, life time of pole. It allows us to understand more correctly
dependence of the system parameters on noise than in arXiv:adap-org/9608001Comment: 23 pages, 4 figures,revised, version accepted for publication in
journal "Combustion, Explosion and Shock Waves". arXiv admin note:
substantial text overlap with arXiv:nlin/0302021, arXiv:adap-org/9608001,
arXiv:nlin/030201
The blue vibronically resolved electroluminescence of azatrioxa[8]circulene
Organic Light Emitting Dioides (OLED)devices were fabricated with blue emission based on azatrioxa[8]circulene and 4,4-N,N'-Dicarbazolyl-1,1'-biphenyl (CBP) with maximum brightness of 840 kd/m(2) at 12 V and the starting voltage of 3.5 V. The vibronic emission spectrum was analyzed by the promotive modes calculation method. The electroluminescence of fabricated OLED device is caused by the 0-0 electronic transition and single excitations of 1473 cm(-1) and 1673 cm(-1) modes and combinations thereof.Peer reviewe
Tight-binding g-Factor Calculations of CdSe Nanostructures
The Lande g-factors for CdSe quantum dots and rods are investigated within
the framework of the semiempirical tight-binding method. We describe methods
for treating both the n-doped and neutral nanostructures, and then apply these
to a selection of nanocrystals of variable size and shape, focusing on
approximately spherical dots and rods of differing aspect ratio. For the
negatively charged n-doped systems, we observe that the g-factors for
near-spherical CdSe dots are approximately independent of size, but show strong
shape dependence as one axis of the quantum dot is extended to form rod-like
structures. In particular, there is a discontinuity in the magnitude of
g-factor and a transition from anisotropic to isotropic g-factor tensor at
aspect ratio ~1.3. For the neutral systems, we analyze the electron g-factor of
both the conduction and valence band electrons. We find that the behavior of
the electron g-factor in the neutral nanocrystals is generally similar to that
in the n-doped case, showing the same strong shape dependence and discontinuity
in magnitude and anisotropy. In smaller systems the g-factor value is dependent
on the details of the surface model. Comparison with recent measurements of
g-factors for CdSe nanocrystals suggests that the shape dependent transition
may be responsible for the observations of anomalous numbers of g-factors at
certain nanocrystal sizes.Comment: 15 pages, 6 figures. Fixed typos to match published versio
Extended Emission from Short Gamma-Ray Bursts Detected with SPI-ACS/INTEGRAL
The short duration (T90 < 2 s) gamma-ray bursts (GRBs) detected in the
SPI-ACS experiment onboard the INTEGRAL observatory are investigated. Averaged
light curves have been constructed for various groups of events, including
short GRBs and unidentified short events. Extended emission has been found in
the averaged light curves of both short GRBs and unidentified short events. It
is shown that the fraction of the short GRBs in the total number of SPI-ACS
GRBs can range from 30 to 45%, which is considerably larger than has been
thought previously.Comment: 27 pages, 10 figure
Existence of radial stationary solutions for a system in combustion theory
In this paper, we construct radially symmetric solutions of a nonlinear
noncooperative elliptic system derived from a model for flame balls with
radiation losses. This model is based on a one step kinetic reaction and our
system is obtained by approximating the standard Arrehnius law by an ignition
nonlinearity, and by simplifying the term that models radiation. We prove the
existence of 2 solutions using degree theory
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