FACTORS RELATED TO SUBSEQUENT ADOLESCENT PREGNANCY IN THE DOMINICAN REPUBLIC

Objectives: Adolescents with subsequent pregnancies in the Dominican Republic represent a significant but poorly understood population with little available data to inform health care services. The objective of this exploratory study was to begin to characterize this important cohort. Methods: A survey of demographic items and a sexual and obstetrical history was administered to 50 adolescents with subsequent pregnancies in La Romana, Dominican Republic. Results: Most participants were married (78%) and exclusively economically dependent on their partner (72%) and nearly half (48%) stopped attending school. Twelve percent reported having intended their current pregnancy. Conclusions: Adolescents with subsequent pregnancies were dependent on partners in the form of living conditions and marriage, economic dependence and unemployment, and lack of education

Progress towards Elimination of HIV Mother-to-Child Transmission in the Dominican Republic from 1999 to 2011

In 1999, prevention of mother-to-child transmission (pMTCT) using antiretrovirals was introduced in the Dominican Republic (DR). Highly active antiretroviral therapy (HAART) was introduced for immunosuppressed persons in 2004 and for pMTCT in 2008. To assess progress towards MTCT elimination, data from requisitions for HIV nucleic acid amplification tests for diagnosis of HIV infection in perinatally exposed infants born in the DR from 1999 to 2011 were analyzed. The MTCT rate was 142/1,274 (11.1%) in 1999?2008 and 12/302 (4.0%) in 2009?2011 (P \u3c .001), with a rate of 154/1,576 (9.8%) for both periods combined. This decline was associated with significant increases in the proportions of women who received prenatal HAART (from 12.3% to 67.9%) and infants who received exclusive formula feeding (from 76.3% to 86.1%) and declines in proportions of women who received no prenatal antiretrovirals (from 31.9% to 12.2%) or received only single-dose nevirapine (from 39.5% to 19.5%). In 2007, over 95% of DR pregnant women received prenatal care, HIV testing, and professionally attended delivery. However, only 58% of women in underserved sugarcane plantation communities (2007) and 76% in HIV sentinel surveillance hospitals (2003?2005) received their HIV test results. HIV-MTCT elimination is feasible but persistent lack of access to critical pMTCT measures must be addressed

Adjuvant Effect of Killed Propionibacterium acnes on Mouse Peritoneal B-1 Lymphocytes and Their Early Phagocyte Differentiation

B-1 lymphocytes are the predominant cells in mouse peritoneal cavity. They express macrophage and lymphocyte markers and are divided into B-1a, B-1b and B-1c subtypes. The role of B-1 cells is not completely clear, but they are responsible for natural IgM production and seem to play a regulatory role. An enriched B-1b cell population can be obtained from non-adherent peritoneal cell cultures, and we have previously demonstrated that these cells undergo differentiation to acquire a mononuclear phagocyte phenotype upon attachment to the substrate in vitro. Nevertheless, the B-1 cell response to antigens or adjuvants has been poorly investigated. Because killed Propionibacterium acnes exhibits immunomodulatory effects on both macrophages and B-2 lymphocytes, we analyzed whether a killed bacterial suspension or its soluble polysaccharide (PS) could modulate the absolute number of peritoneal B-1 cells in BALB/c mice, the activation status of these cells and their ability to differentiate into phagocytes in vitro. In vivo, P. acnes treatment elevated the absolute number of all B-1 subsets, whereas PS only increased B-1c. Moreover, the bacterium increased the number of B-1b cells that were positive for MHC II, TLR2, TLR4, TLR9, IL-4, IL-5 and IL-12, in addition to up-regulating TLR9, CD80 and CD86 expression. PS increased B-1b cell expression of TLR4, TLR9, CD40 and CD86, as well as IL-10 and IL-12 synthesis. Both of the treatments decreased the absolute number of B-1b cells in vitro, suggesting their early differentiation into B-1 cell-derived phagocytes (B-1CDP). We also observed a higher phagocytic activity from the phagocytes that were derived from B-1b cells after P. acnes and PS treatment. The adjuvant effect that P. acnes has on B-1 cells, mainly the B-1b subtype, reinforces the importance of B-1 cells in the innate and adaptive immune responses

Assessment of risk factors for sexually transmitted infections in high-risk communities in Santo Domingo and La Romana, Dominican Republic

Background: This study assesses the prevalence of sexually transmitted infections and risk factors among key populations living in La Romana and Santo Domingo, Dominican Republic: pregnant adolescents, men who have sex with men, trans women, sugar cane field (batey) residents, transactional sex workers, and people living with HIV. This work is based on a preliminary analysis of batey data collected in La Romana. Methods: In 2017, 203 participants, aged 18–60 years, were recruited from eight bateys systematically selected using time-location sampling. Informed consent was obtained, and a 40 min questionnaire was administered. Blood, urine, Papanicolaou samples, and oral/anal swabs were used to test for HIV, syphilis, hepatitis B virus (HBV), hepatitis C virus (HCV), gonorrhoea, chlamydia, Trichomonas vaginalis, Ureaplasma spp, and Mycoplasma genitalium, and Papanicolaou (cervical) samples were collected. Participants with positive results received free treatment, when available, follow-up and education. Findings: Of 203 participants, 131 (65%) were women and 161 (79%) were Spanish-speaking. One (<1%) participant tested HIV-positive, 32 (16%) participants were chlamydia-positive, and 18 (9%) participants were anal chlamydia positive. 27 (13%) participants were positive for T vaginalis, and 60 (30%) participants were positive for Ureaplasma spp. 116 (88%) of 132 women underwent Papanicolaou testing. 21 (18%) women had abnormal Papanicolaou results, and 36 (31%) women were positive for human papillomavirus (HPV); of those, 25% of women had HPV16, 8% of women had HPV18/45, and 67% of women had indeterminate high-risk type. 19 (9%) participants were HBV positive. Two bateys were disproportionately affected: nine (33%) of 27 participants in Batey 1 and eight (32%) of 25 participants in Batey 2. Interpretation: The prevalence of HBV infection was disproportionately higher than those found in Central and Latin America. In response, Clínica de Familia La Romana and the Dominican Ministry of Health established an HBV vaccination and prevention programme for affected bateys, and this is underway. In terms of HIV, the prevalence was lower (<1% vs 3%) when compared to that previously reported in bateys. Targeted HIV prevention programmes conducted by Clínica de Familia La Romana and others might have helped curb HIV infection. The prevalence of high-risk HPV and other sexually transmitted infections in this study needs to be further explored. Overall, these results highlight the need for more research, for targeted prevention initiatives, and improved access to testing and treatment for sexually transmitted infections. Funding: David E Rogers Fellowship, NY Academy of Medicine Bioreference Laboratories

Thoughts of harm (n = 49).

Violence against adolescents is a pressing health problem with long-term implications for future physical and mental well-being, such as thoughts of self-harm, which have been associated with suicidal ideation and completion. However, much of the research has been conducted only in high-income countries. This study aimed to examine the correlation between violence against adolescents and self-harm thoughts in La Romana, Dominican Republic (DR). Cross-sectional survey data was collected at a community-based clinic from participants aged 13–20. Participants were recruited through the clinic’s adolescent program and peer referral, and verbal consent was obtained. A survey solicited information about each participant’s demographics, experiences with violence, and thoughts of harm to self or others. The survey was completed by 49 adolescents. The mean age was 16.78 (SD 2.34); 65% were female. We performed t-tests and Fisher’s exact to investigate the relationship between demographics, reported violence experiences and having self-harm thoughts. About half (45%) had experienced physical violence, 76% had experienced emotional violence, and 12% had experienced sexual violence. The most common perpetrators of physical and emotional violence were classmates (12% and 24%), and the most common perpetrator of sexual violence was an ex-partner (4.1%). Ten participants (20.4%) had thought about harming themselves. Self-harm thoughts were significantly associated with being female (p = 0.025), employed (p = 0.05), and to a higher number of experiences of physical (0.029) and sexual violence (p = 0.023). The results of this study suggest a high prevalence of both violence and self-harm thoughts in adolescents in the DR. Interventions that address physical and sexual violence against adolescents may be particularly important. Particular attention should also be paid to screening for self-harm thoughts in female-identifying adolescents. Further research is needed to better understand the relationship between violence and self-harm thoughts in adolescents in the DR.</div

Sample characteristics (n = 49).

Violence against adolescents is a pressing health problem with long-term implications for future physical and mental well-being, such as thoughts of self-harm, which have been associated with suicidal ideation and completion. However, much of the research has been conducted only in high-income countries. This study aimed to examine the correlation between violence against adolescents and self-harm thoughts in La Romana, Dominican Republic (DR). Cross-sectional survey data was collected at a community-based clinic from participants aged 13–20. Participants were recruited through the clinic’s adolescent program and peer referral, and verbal consent was obtained. A survey solicited information about each participant’s demographics, experiences with violence, and thoughts of harm to self or others. The survey was completed by 49 adolescents. The mean age was 16.78 (SD 2.34); 65% were female. We performed t-tests and Fisher’s exact to investigate the relationship between demographics, reported violence experiences and having self-harm thoughts. About half (45%) had experienced physical violence, 76% had experienced emotional violence, and 12% had experienced sexual violence. The most common perpetrators of physical and emotional violence were classmates (12% and 24%), and the most common perpetrator of sexual violence was an ex-partner (4.1%). Ten participants (20.4%) had thought about harming themselves. Self-harm thoughts were significantly associated with being female (p = 0.025), employed (p = 0.05), and to a higher number of experiences of physical (0.029) and sexual violence (p = 0.023). The results of this study suggest a high prevalence of both violence and self-harm thoughts in adolescents in the DR. Interventions that address physical and sexual violence against adolescents may be particularly important. Particular attention should also be paid to screening for self-harm thoughts in female-identifying adolescents. Further research is needed to better understand the relationship between violence and self-harm thoughts in adolescents in the DR.</div