Recent glacial recession in the Rwenzori Mountains of East Africa due to rising air temperature

Based on field surveys and analyses of optical spaceborne images (LandSat5, LandSat7), we report recent decline in the areal extent of glaciers in the Rwenzori Mountains of East Africa from 2.01 +/- 0.56 km(2) in 1987 to 0.96 +/- 0.34 km(2) in 2003. The spatially uniform loss of glacial cover at lower elevations together with meteorological trends derived from both station and reanalysis data, indicate that increased air temperature is the main driver. Clear trends toward increased air temperatures over the last four decades of similar to 0.5 degrees C per decade exist without significant changes in annual precipitation. Extrapolation of trends in glacial recession since 1906 suggests that glaciers in the Rwenzori Mountains will disappear within the next two decades

Hydrological and climatological change associated with glacial recession in the Rwenzori Mountains of Uganda

The areal extent of tropical icefields in the Rwenzori Mountains of East Africa has reduced steadily over the last century from 7.5 km^{2} 2 in 1906 to <1 km^{2} in 2003. Considerable debate persists regarding the impact of deglaciation on alpine riverflow and changes in climate driving glacial recession in the East African Highlands. Recent field surveys combined with historical observations reveal continued, rapid retreat in the terminal positions of valley glaciers (Speke, Elena). Observed acceleration in the rate of termini retreat since the 1960s is shown to arise, in part, from the morphologies of the glaciers and the beds within which those glaciers reside. Historical data combined with the first measurements of alpine riverflow in the Rwenzori Mountains show that the contribution of meltwater flows from dwindling icefields to alpine riverflow is negligible, contributing <0.5% of the mean annual river discharge recorded at the base of the mountains. Preliminary high-frequency monitoring of air temperature and humidity in the vicinity of icefields on the Rwenzori Mountains indicates that elevated daily maximum air temperatures coincide with episodic reductions in relative humidity and increased meltwater fluxes observed during the dry season. A sustained reduction in humidity to account for observed deglaciation is not evident from records of lowland precipitation, humidity or river discharge. Lakelevel records in East Africa are also inconsistent with a sudden decrease in regional humidity around 1880AD that is proposed to have triggered deglaciation in the East African Highlands. Water levels in the lakes proximate to the icefields of Mount Kenya and Kilimanjaro are rising in the late 19th century when glaciers on these mountains are observed to be in retreat. Lake levels do not, furthermore, indicate that enhanced humidity over the 19th century prior to 1880AD relative to the 20th century. Evidence of warming over the latter half of the 20th century and an earlier onset of deglaciation (~1870AD) from meteorological and palaeolimnological data suggest that the timing and drivers of deglaciation in the Rwenzori Mountains are consistent with the recession of alpine icefields elsewhere in the tropics

Climate change and the aquatic ecosystems of the Rwenzori Mountains, Uganda

The Rwenzori Mountains are home to one of the last remaining tropical icefields outside of the Andes. Over the last century, equatorial icefields of the East African highlands have been steadily shrinking but the precise climate tropical alpine glaciers remain unclear. More than a decade had passed since the last detailed measurements of glacial cover were made in the Rwenzori Mountains. Recent evidence from Kilimanjaro suggests that its icecap will disappear entirely by the year 2020(1). The Rwenzori glaciers contribute meltwater flows to aquatic ecosystems of the Rwenzori Mountains National Park, a Word Heritage Site featuring spectacular, rare Afroalpine flora and fauna, and are headwaters of the River Nile. With the overall aim of assessing the impact of recent climate change on alpine aquatic ecosystems of the Rwenzori Mountains, a collaborative, international research team led by the University College London (United Kingdom) and Makerere University (Uganda), and involving the Institut für Geographie from the University of Innsbruck (Austria) and Water Resources Management Department (Uganda) was assembled in order to pursue three primary scientific objectives: • to assess the magnitude of current glacial recession; • to assess the impact of glacial recession on alpine riverflow; and • to assess recent environmental change from observational datasets and available, environmental archives stored in lake sediment and glacial ice

Design and development of exome capture sequencing for the domestic pig (Sus scrofa)

Background: The domestic pig (Sus scrofa) is both an important livestock species and a model for biomedical research. Exome sequencing has accelerated identification of protein-coding variants underlying phenotypic traits in human and mouse. We aimed to develop and validate a similar resource for the pig.Results: We developed probe sets to capture pig exonic sequences based upon the current Ensembl pig gene annotation supplemented with mapped expressed sequence tags (ESTs) and demonstrated proof-of-principle capture and sequencing of the pig exome in 96 pigs, encompassing 24 capture experiments. For most of the samples at least 10x sequence coverage was achieved for more than 90% of the target bases. Bioinformatic analysis of the data revealed over 236,000 high confidence predicted SNPs and over 28,000 predicted indels.Conclusions: We have achieved coverage statistics similar to those seen with commercially available human and mouse exome kits. Exome capture in pigs provides a tool to identify coding region variation associated with production traits, including loss of function mutations which may explain embryonic and neonatal losses, and to improve genomic assemblies in the vicinity of protein coding genes in the pig

Referenceless, grating-based, single shot X-ray phase contrast imaging with optimized laser-driven K-α sources

Producción CientíficaWith its ability to efficiently probe low-Z materials, X-ray phase imaging methods have recently raised high interest in multiple fields from biology and medical applications to high energy density (HED) physics. Initially developed with synchrotron light and X-ray tubes, we present a novel grating based Talbot X-ray deflectometer (TXD) diagnostic that was coupled with laser-generated K-α X-ray sources. The multi-terawatt laser (I > 1 × 1014 W cm-2) was used as a testbed for diagnostic development. It was found that X-ray source chromaticity plays an important role in TXD. Indeed, the broadband spectrum of laser-generated X-ray sources may strongly impact image quality and thus diagnostic performance. We qualified X-ray emission from different laser-produced sources and determined laser, target, and deflectometer parameters that optimize TXD performance. We present the first results of referenceless grating-based X-ray imaging at high-power laser facilities and discuss the implications of this new development in HED research.This work has also been supported by the Research Grants No. PID2019-108764RB-I00 and PID2022-137632OB-I00 from the Spanish Ministry of Science and Innovation

Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function

Porcine Reproductive and Respiratory Syndrome (PRRS) is a panzootic infectious disease of pigs, causing major economic losses to the world-wide pig industry. PRRS manifests differently in pigs of all ages but primarily causes late-term abortions and stillbirths in sows and respiratory disease in piglets. The causative agent of the disease is the positive-strand RNA PRRS virus (PRRSV). PRRSV has a narrow host cell tropism, limited to cells of the monocyte/macrophage lineage. CD163 has been described as a fusion receptor for PRRSV, whereby the scavenger receptor cysteine-rich domain 5 (SRCR5) region was shown to be an interaction site for the virus in vitro. CD163 is expressed at high levels on the surface of macrophages, particularly in the respiratory system. Here we describe the application of CRISPR/Cas9 to pig zygotes, resulting in the generation of pigs with a deletion of Exon 7 of the CD163 gene, encoding SRCR5. Deletion of SRCR5 showed no adverse effects in pigs maintained under standard husbandry conditions with normal growth rates and complete blood counts observed. Pulmonary alveolar macrophages (PAMs) and peripheral blood monocytes (PBMCs) were isolated from the animals and assessed in vitro. Both PAMs and macrophages obtained from PBMCs by CSF1 stimulation (PMMs) show the characteristic differentiation and cell surface marker expression of macrophages of the respective origin. Expression and correct folding of the SRCR5 deletion CD163 on the surface of macrophages and biological activity of the protein as hemoglobin-haptoglobin scavenger was confirmed. Challenge of both PAMs and PMMs with PRRSV genotype 1, subtypes 1, 2, and 3 and PMMs with PRRSV genotype 2 showed complete resistance to viral infections assessed by replication. Confocal microscopy revealed the absence of replication structures in the SRCR5 CD163 deletion macrophages, indicating an inhibition of infection prior to gene expression, i.e. at entry/fusion or unpacking stages