Evaluation of the analytical performances of the Cobas c513 analyser for HbA1c assay

Introduction: Haemoglobin A1c (HbA1c) is considered to be the gold standard for the follow-up of glycaemic control in patients with diabetes mellitus and is also a diagnostic tool. Accordingly, reliable and efficient methods must be used for its quantification. Roche Diagnostics have recently adapted the Tina-quant® HbA1c Third Generation immunoassay on a fully dedicated analyser, the Cobas c513, which allows a high throughput of up to 400 samples per hour. The present article deals with the evaluation of the analytical performances of this system which has been recently introduced to the market. Materials and methods: Precision, comparison with two ion-exchange high-performance liquid chromatography (HPLC) methods (Variant II and D-100 systems, BioRad Laboratories) using Passing Bablok and Bland-Altman analyses, accuracy and interference of the most frequent haemoglobin (Hb) variants on HbA1c measurement were evaluated. Results: Precision was high, with coefficients of variation lower than 1.1% (HbA1c values expressed in National Glycohemoglobin Standardization Program units, 1.7% for values expressed in International Federation of Clinical Chemistry and Laboratory Medicine [IFCC] units). The comparison study showed similar results with the two HPLC systems. The analysis of samples with IFCC-assigned values showed high methodological accuracy. Finally, no interference of bilirubin, triglycerides and common Hb variants (Hb AC, AD, AE, AS) was observed. Conclusions: This evaluation showed that the analytical performance of the Cobas c513 analyser for HbA1c assay makes it suitable for a routine use in clinical chemistry laboratories

Spectroimmunohistochemistry: A Novel Form of MALDI Mass Spectrometry Imaging Coupled to Immunohistochemistry for Tracking Antibodies.

Peer reviewe

Multiple Changes in Peptide and Lipid Expression Associated with Regeneration in the Nervous System of the Medicinal Leech

peer reviewe

Ovarian cancer molecular pathology.

Peer reviewe

Ophiolites and melange terranes in Iran: a geochronological study and its paleotectonic implications

In Iran, units mapped as “coloured melange zones” consist of either melange terranes, ophiolite complexes, or ultramafic—mafic units, which are not complete ophiolitic sequences. These zones represent block margins that were active in Alpine times. Crystallization of the ophiolitic rocks, their emplacement at continental margins and the mixing of various rocks in melange are post-Paleozoic events that were considered from stratigraphical data to be pre-Upper Cretaceous to Cenozoic in age. Thirteen whole-rock and 30 mineral K-Ar ages were obtained from the ophiolitic complexes and from igneous and metamorphic rocks in the melange zones. Ophiolite crystallization, ocean-floor metamorphism and blueschist metamorphism in the south Baluchestan rocks appear to be of Campanian age. Ocean crust generation occurred at the same time in the Zagros Belt, but is perhaps slightly older in the East Iranian Ranges (north Baluchestan). Middle-grade metamorphic alteration of the exotic rocks of south Baluchestan and south Iran occurred at some time between (?) Late Jurassic to Late Cretaceous times (south Iran) and in the Campanian (south Baluchestan). There is evidence for an Early Eocene low-grade metamorphism in Baluchestan, perhaps of the same age as that in the Zagros Belt. Alkaline to calc-alkaline magmatism in the melange domain of north Baluchestan is Middle—Late Eocene, and Aquitanian. The formation of the melange, stratigraphically of Upper Cretaceous age in the Zagros Belt, may have occurred at the Cretaceous-Paleocene boundary in Baluchestan and continued possibly into post-Eocene times

K-Ar Radiometric Age Determinations of White Micas from the Piemont Zone, French-Italian Western Alps

K - Ar radiometric data are presented suggesting a different metamorphic history for both parts of the Piemont zone in the Western Alps: the Combin zone to the west, considered to have escaped the late Cretaceous metamorphic event, and the Zermatt-Saas zone to the east, where ophiolites predominate, and which has been affected by a first metamorphism during late Cretaceous. Paragonite lenses within serpentinite give an isotopic ratio dating a serpentinization phase, or representing mixed ages. Late Cretaceous ages are found in the Zermatt-Saas zone and in the melange areas. The Combin zone gives only Eocene-Oligocene ages, as well as that part of the Zermatt-Saas zone that is closer to the Lepontine thermal high (dated as 38 m.y.). No late Oligocene-early Miocene ages (as previously obtained on Na-amphiboles) have been found among the investigated micas

Rôles du chirurgien dentiste dans la prise en charge des cancers épidermoïdes de la langue mobile

LILLE2-BU Santé-Recherche (593502101) / SudocPARIS-BIUM (751062103) / SudocLILLE2-UFR Odontologie (593502202) / SudocSudocFranceF