576 research outputs found
Brain Activations to Emotional Pictures are Differentially Associated with Valence and Arousal Ratings
Several studies have investigated the neural responses triggered by emotional pictures, but the specificity of the involved structures such as the amygdala or the ventral striatum is still under debate. Furthermore, only few studies examined the association of stimuli's valence and arousal and the underlying brain responses. Therefore, we investigated brain responses with functional magnetic resonance imaging of 17 healthy participants to pleasant and unpleasant affective pictures and afterwards assessed ratings of valence and arousal. As expected, unpleasant pictures strongly activated the right and left amygdala, the right hippocampus, and the medial occipital lobe, whereas pleasant pictures elicited significant activations in left occipital regions, and in parts of the medial temporal lobe. The direct comparison of unpleasant and pleasant pictures, which were comparable in arousal clearly indicated stronger amygdala activation in response to the unpleasant pictures. Most important, correlational analyses revealed on the one hand that the arousal of unpleasant pictures was significantly associated with activations in the right amygdala and the left caudate body. On the other hand, valence of pleasant pictures was significantly correlated with activations in the right caudate head, extending to the nucleus accumbens (NAcc) and the left dorsolateral prefrontal cortex. These findings support the notion that the amygdala is primarily involved in processing of unpleasant stimuli, particularly to more arousing unpleasant stimuli. Reward-related structures like the caudate and NAcc primarily respond to pleasant stimuli, the stronger the more positive the valence of these stimuli is
High stakes and low bars: How international recognition shapes the conduct of civil wars
When rebel groups engage incumbent governments in war for control of the state, questions of international recognition arise. International recognition determines which combatants can draw on state assets, receive overt military aid, and borrow as sovereigns—all of which can have profound consequences for the military balance during civil war. How do third-party states and international organizations determine whom to treat as a state's official government during civil war? Data from the sixty-one center-seeking wars initiated from 1945 to 2014 indicate that military victory is not a prerequisite for recognition. Instead, states generally rely on a simple test: control of the capital city. Seizing the capital does not foreshadow military victory. Civil wars often continue for many years after rebels take control and receive recognition. While geopolitical and economic motives outweigh the capital control test in a small number of important cases, combatants appear to anticipate that holding the capital will be sufficient for recognition. This expectation generates perverse incentives. In effect, the international community rewards combatants for capturing or holding, by any means necessary, an area with high concentrations of critical infrastructure and civilians. In the majority of cases where rebels contest the capital, more than half of its infrastructure is damaged or the majority of civilians are displaced (or both), likely fueling long-term state weakness
ArborZ: Photometric Redshifts Using Boosted Decision Trees
Precision photometric redshifts will be essential for extracting cosmological
parameters from the next generation of wide-area imaging surveys. In this paper
we introduce a photometric redshift algorithm, ArborZ, based on the
machine-learning technique of Boosted Decision Trees. We study the algorithm
using galaxies from the Sloan Digital Sky Survey and from mock catalogs
intended to simulate both the SDSS and the upcoming Dark Energy Survey. We show
that it improves upon the performance of existing algorithms. Moreover, the
method naturally leads to the reconstruction of a full probability density
function (PDF) for the photometric redshift of each galaxy, not merely a single
"best estimate" and error, and also provides a photo-z quality figure-of-merit
for each galaxy that can be used to reject outliers. We show that the stacked
PDFs yield a more accurate reconstruction of the redshift distribution N(z). We
discuss limitations of the current algorithm and ideas for future work.Comment: 10 pages, 13 figures, submitted to Ap
The DECam Ecliptic Exploration Project (DEEP) II. Observational Strategy and Design
We present the DECam Ecliptic Exploration Project (DEEP) survey strategy
including observing cadence for orbit determination, exposure times, field
pointings and filter choices. The overall goal of the survey is to discover and
characterize the orbits of a few thousand Trans-Neptunian Objects (TNOs) using
the Dark Energy Camera (DECam) on the Cerro Tololo Inter-American Observatory
(CTIO) Blanco 4 meter telescope. The experiment is designed to collect a very
deep series of exposures totaling a few hours on sky for each of several 2.7
square degree DECam fields-of-view to achieve a magnitude of about 26.2 using a
wide VR filter which encompasses both the V and R bandpasses. In the first
year, several nights were combined to achieve a sky area of about 34 square
degrees. In subsequent years, the fields have been re-visited to allow TNOs to
be tracked for orbit determination. When complete, DEEP will be the largest
survey of the outer solar system ever undertaken in terms of newly discovered
object numbers, and the most prolific at producing multi-year orbital
information for the population of minor planets beyond Neptune at 30 au.Comment: 29 pages, 4 figures and 4 table
Large Synoptic Survey Telescope Solar System Science Roadmap
The Large Synoptic Survey Telescope (LSST) is uniquely equipped to search for
Solar System bodies due to its unprecedented combination of depth and wide
field coverage. Over a ten-year period starting in 2022, LSST will generate the
largest catalog of Solar System objects to date. The main goal of the LSST
Solar System Science Collaboration (SSSC) is to facilitate the efforts of the
planetary community to study the planets and small body populations residing
within our Solar System using LSST data. To prepare for future survey cadence
decisions and ensure that interesting and novel Solar System science is
achievable with LSST, the SSSC has identified and prioritized key Solar System
research areas for investigation with LSST in this roadmap. The ranked science
priorities highlighted in this living document will inform LSST survey cadence
decisions and aid in identifying software tools and pipelines needed to be
developed by the planetary community as added value products and resources
before the planned start of LSST science operations.Comment: 7 pages; Feedback welcom
Excess PLAC8 promotes an unconventional ERK2-dependent EMT in colon cancer
The epithelial-to-mesenchymal transition (EMT) transcriptional program is characterized by repression of E-cadherin (CDH1) and induction of N-cadherin (CDH2), and mesenchymal genes like vimentin (VIM). Placenta-specific 8 (PLAC8) has been implicated in colon cancer; however, how PLAC8 contributes to disease is unknown, and endogenous PLAC8 protein has not been studied. We analyzed zebrafish and human tissues and found that endogenous PLAC8 localizes to the apical domain of differentiated intestinal epithelium. Colon cancer cells with elevated PLAC8 levels exhibited EMT features, including increased expression of VIM and zinc finger E-box binding homeobox 1 (ZEB1), aberrant cell motility, and increased invasiveness. In contrast to classical EMT, PLAC8 overexpression reduced cell surface CDH1 and upregulated P-cadherin (CDH3) without affecting CDH2 expression. PLAC8-induced EMT was linked to increased phosphorylated ERK2 (p-ERK2), and ERK2 knockdown restored cell surface CDH1 and suppressed CDH3, VIM, and ZEB1 upregulation. In vitro, PLAC8 directly bound and inactivated the ERK2 phosphatase DUSP6, thereby increasing p-ERK2. In a murine xenograft model, knockdown of endogenous PLAC8 in colon cancer cells resulted in smaller tumors, reduced local invasion, and decreased p-ERK2. Using MultiOmyx, a multiplex immunofluorescence-based methodology, we observed coexpression of cytosolic PLAC8, CDH3, and VIM at the leading edge of a human colorectal tumor, supporting a role for PLAC8 in cancer invasion in vivo
Multi-Level Communication of Human Retinal Pigment Epithelial Cells via Tunneling Nanotubes
Background: Tunneling nanotubes (TNTs) may offer a very specific and effective way of intercellular communication. Here we investigated TNTs in the human retinal pigment epithelial (RPE) cell line ARPE-19. Morphology of TNTs was examined by immunostaining and scanning electron microscopy. To determine the function of TNTs between cells, we studied the TNT-dependent intercellular communication at different levels including electrical and calcium signalling, small molecular diffusion as well as mitochondrial re-localization. Further, intercellular organelles transfer was assayed by FACS analysis. Methodology and Principal Findings: Microscopy showed that cultured ARPE-19 cells are frequently connected by TNTs, which are not attached to the substratum. The TNTs were straight connections between cells, had a typical diameter of 50 to 300 nm and a length of up to 120 µm. We observed de novo formation of TNTs by diverging from migrating cells after a short time of interaction. Scanning electron microscopy confirmed characteristic features of TNTs. Fluorescence microscopy revealed that TNTs between ARPE-19 cells contain F-actin but no microtubules. Depolymerisation of F-actin, induced by addition of latrunculin-B, led to disappearance of TNTs. Importantly, these TNTs could function as channels for the diffusion of small molecules such as Lucifer Yellow, but not for large molecules like Dextran Red. Further, organelle exchange between cells via TNTs was observed by microscopy. Using Ca2+ imaging we show the intercellular transmission of calcium signals through TNTs. Mechanical stimulation led to membrane depolarisation, which expand through TNT connections between ARPE-19 cells. We further demonstrate that TNTs can mediate electrical coupling between distant cells. Immunolabelling for Cx43 showed that this gap junction protein is interposed at one end of 44% of TNTs between ARPE-19 cells. Conclusions and Significance: Our observations indicate that human RPE cell line ARPE-19 cells communicate by tunneling nanotubes and can support different types of intercellular traffic
The DEEP2 Galaxy Redshift Survey: Design, Observations, Data Reduction, and Redshifts
We describe the design and data sample from the DEEP2 Galaxy Redshift Survey,
the densest and largest precision-redshift survey of galaxies at z ~ 1
completed to date. The survey has conducted a comprehensive census of massive
galaxies, their properties, environments, and large-scale structure down to
absolute magnitude M_B = -20 at z ~ 1 via ~90 nights of observation on the
DEIMOS spectrograph at Keck Observatory. DEEP2 covers an area of 2.8 deg^2
divided into four separate fields, observed to a limiting apparent magnitude of
R_AB=24.1. Objects with z < 0.7 are rejected based on BRI photometry in three
of the four DEEP2 fields, allowing galaxies with z > 0.7 to be targeted ~2.5
times more efficiently than in a purely magnitude-limited sample. Approximately
sixty percent of eligible targets are chosen for spectroscopy, yielding nearly
53,000 spectra and more than 38,000 reliable redshift measurements. Most of the
targets which fail to yield secure redshifts are blue objects that lie beyond z
~ 1.45. The DEIMOS 1200-line/mm grating used for the survey delivers high
spectral resolution (R~6000), accurate and secure redshifts, and unique
internal kinematic information. Extensive ancillary data are available in the
DEEP2 fields, particularly in the Extended Groth Strip, which has evolved into
one of the richest multiwavelength regions on the sky. DEEP2 surpasses other
deep precision-redshift surveys at z ~ 1 in terms of galaxy numbers, redshift
accuracy, sample number density, and amount of spectral information. We also
provide an overview of the scientific highlights of the DEEP2 survey thus far.
This paper is intended as a handbook for users of the DEEP2 Data Release 4,
which includes all DEEP2 spectra and redshifts, as well as for the
publicly-available DEEP2 DEIMOS data reduction pipelines. [Abridged]Comment: submitted to ApJS; data products available for download at
http://deep.berkeley.edu/DR4
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