46 research outputs found
Insights into Eyestalk Ablation Mechanism to Induce Ovarian Maturation in the Black Tiger Shrimp
Eyestalk ablation is commonly practiced in crustacean to induce ovarian maturation in captivity. The molecular mechanism of the ablation has not been well understood, preventing a search for alternative measures to induce ovarian maturation in aquaculture. This is the first study to employ cDNA microarray to examine effects of eyestalk ablation at the transcriptomic level and pathway mapping analysis to identify potentially affected biological pathways in the black tiger shrimp (Penaeus monodon). Microarray analysis comparing between gene expression levels of ovaries from eyestalk-intact and eyestalk-ablated brooders revealed 682 differentially expressed transcripts. Based on Hierarchical clustering of gene expression patterns, Gene Ontology annotation, and relevant functions of these differentially expressed genes, several gene groups were further examined by pathway mapping analysis. Reverse-transcriptase quantitative PCR analysis for some representative transcripts confirmed microarray data. Known reproductive genes involved in vitellogenesis were dramatically increased during the ablation. Besides these transcripts expected to be induced by the ablation, transcripts whose functions involved in electron transfer mechanism, immune responses and calcium signal transduction were significantly altered following the ablation. Pathway mapping analysis revealed that the activation of gonadotropin-releasing hormone signaling, calcium signaling, and progesterone-mediated oocyte maturation pathways were putatively crucial to ovarian maturation induced by the ablation. These findings shed light on several possible molecular mechanisms of the eyestalk ablation effect and allow more focused investigation for an ultimate goal of finding alternative methods to replace the undesirable practice of the eyestalk ablation in the future
Ablação ocular no camarão Macrobrachium rosenbergii (De Man) (Crustacea, Decapoda, Palaemonidae): efeitos sobre a reprodução, pigmentação epidérmica e atividade alimentar
Vitellogenin, the extraovarian precursor of the protein yolk in Crustacea : A review
International audienc
La vitellogénine chez un Crustacé Décapode Natantia, Palaemon serratus Pennant. Mise en évidence, comparaison immunologique avec les vitellines, site de synthèse et rôle des pédoncules oculaires
International audienc
De Euripide sophista : thesis philosophica / quam... proponit... Ed.-J.-B. Meusy,...
Avec mode text
Des moeurs dans la tragédie grecque : thèse de littérature / par Ed.-J.-B. Meusy
Avec mode text
Relationships between Leydig cell morphometry and plasma testosterone during postnatal development of the monkey, Macaca fascicularis
International audienc
Relationships between Leydig cell morphometry and plasma testosterone during postnatal development of the monkey, Macaca fascicularis
Functional differentiation of Leydig cells in the testis of the fetal monkey (Macaca fascicularis)
International audienc
Quelques aspects du contrôle hormonal de la synthèse de la vitellogénine chez le crustacé amphipode Orchestia gammarella (Pallas)
International audienc