La construction d’une démarche interdisciplinaire à partir de l’émergence de la céréaliculture biologique en Camargue: le projet CEBIOCA

La céréaliculture biologique apparait comme une alternative aux modes de production conventionnels, pour un nombre croissant d'agriculteurs de Camargue. Le programme CEBIOCA cherche à appuyer le développement de ce prototype d'agriculture en favorisant l'élaboration de pratiques agricoles pertinentes, la production de matériel végétal adapté et la mesure des impacts de la conversion à l'AB. Un groupe de travail interdisciplinaire, associant génétique, agronomie, malherbologie, écologie et sociologie s'est constitué à l'occasion de la réponse à l'appel d'offre CIAB et au cours de la première phase de fonctionnemet qui est présenté ici. Malgré certaines lacunes ou faiblesses dans la construction interdiciplinaire, les résultats font entrevoir des perspectives prometteuses,dans un contexte plus favorable, à condition que les partenariats scientifiques et professionnles soient renforcés

Calibration test of PET scanners in a multi-centre clinical trial on breast cancer therapy monitoring using 18F-FLT.

UNLABELLED: A multi-centre trial using PET requires the analysis of images acquired on different systems We designed a multi-centre trial to estimate the value of 18F-FLT-PET to predict response to neoadjuvant chemotherapy in patients with newly diagnosed breast cancer. A calibration check of each PET-CT and of its peripheral devices was performed to evaluate the reliability of the results. MATERIAL AND METHODS: 11 centres were investigated. Dose calibrators were assessed by repeated measurements of a 68Ge certified source. The differences between the clocks associated with the dose calibrators and inherent to the PET systems were registered. The calibration of PET-CT was assessed with an homogeneous cylindrical phantom by comparing the activities per unit of volume calculated from the dose calibrator measurements with that measured on 15 Regions of Interest (ROIs) drawn on 15 consecutive slices of reconstructed filtered back-projection (FBP) images. Both repeatability of activity concentration based upon the 15 ROIs (ANOVA-test) and its accuracy were evaluated. RESULTS: There was no significant difference for dose calibrator measurements (median of difference -0.04%; min = -4.65%; max = +5.63%). Mismatches between the clocks were less than 2 min in all sites and thus did not require any correction, regarding the half life of 18F. For all the PET systems, ANOVA revealed no significant difference between the activity concentrations estimated from the 15 ROIs (median of difference -0.69%; min = -9.97%; max = +9.60%). CONCLUSION: No major difference between the 11 centres with respect to calibration and cross-calibration was observed. The reliability of our 18F-FLT multi-centre clinical trial was therefore confirmed from the physical point of view. This type of procedure may be useful for any clinical trial involving different PET systems

Geometagenomics illuminates the impact of agriculture on the distribution and prevalence of plant viruses at the ecosystem scale

© 2018 International Society for Microbial Ecology All rights reserved. Disease emergence events regularly result from human activities such as agriculture, which frequently brings large populations of genetically uniform hosts into contact with potential pathogens. Although viruses cause nearly 50% of emerging plant diseases, there is little systematic information about virus distribution across agro-ecological interfaces and large gaps in understanding of virus diversity in nature. Here we applied a novel landscape-scale geometagenomics approach to examine relationships between agricultural land use and distributions of plant-associated viruses in two Mediterranean-climate biodiversity hotspots (Western Cape region of South Africa and Rhône river delta region of France). In total, we analysed 1725 geo-referenced plant samples collected over two years from 4.5 × 4.5 km 2 grids spanning farmlands and adjacent uncultivated vegetation. We found substantial virus prevalence (25.8-35.7%) in all ecosystems, but prevalence and identified family-level virus diversity were greatest in cultivated areas, with some virus families displaying strong agricultural associations. Our survey revealed 94 previously unknown virus species, primarily from uncultivated plants. This is the first effort to systematically evaluate plant-associated viromes across broad agro-ecological interfaces. Our findings indicate that agriculture substantially influences plant virus distributions and highlight the extent of current ignorance about the diversity and roles of viruses in nature

Geometagenomics illuminates the impact of agriculture on the distribution and prevalence of plant viruses at the ecosystem scale

Disease emergence events regularly result from human activities such as agriculture, which frequently brings large populations of genetically uniform hosts into contact with potential pathogens. Although viruses cause nearly 50% of emerging plant diseases, there is little systematic information about virus distribution across agro-ecological interfaces and large gaps in understanding of virus diversity in nature. Here we applied a novel landscape-scale geometagenomics approach to examine relationships between agricultural land use and distributions of plantassociated viruses in two Mediterranean-climate biodiversity hotspots (Western Cape region of South Africa and Rhône river delta region of France). In total, we analysed 1725 geo-referenced plant samples collected over two years from 4.5 × 4.5 km2 grids spanning farmlands and adjacent uncultivated vegetation. We found substantial virus prevalence (25.8–35.7%) in all ecosystems, but prevalence and identified family-level virus diversity were greatest in cultivated areas, with some virus families displaying strong agricultural associations. Our survey revealed 94 previously unknown virus species, primarily from uncultivated plants. This is the first effort to systematically evaluate plant-associated viromes across broad agro-ecological interfaces. Our findings indicate that agriculture substantially influences plant virus distributions and highlight the extent of current ignorance about the diversity and roles of viruses in nature

Photoperiod buffers responses to salt and temperature during germination of two coastal salt marsh colonizers Juncus acutus and Juncus maritimus

International audienceJuncus acutus and J. maritimus are two colonizers of coastal marsh rangeland with worldwide distribution. We tested the effect of salt, temperature, and photoperiod on the germination capacity of seeds of the two Juncus from the Rhone delta (south of France). We measured the first day of germination, the mean time, speed, and rate of germination on seeds subjected to five salinity levels, three Dark-Light temperatures and two D-L photoperiods (12-12 and 10-14). The 10-14 D-L photoperiod, although it corresponds to one of the two main germination periods of J. acutus and J. maritimus in the northern Mediterranean, has never been previously used to study their germination capacity. Analyses showed significant effects of salinity and temperature on the germination parameters tested, and for these two factors we found results comparable to those of previous studies. Salinity slowed down and reduced the germination process while spring temperatures had a positive effect. A surprising result was obtained by changing the D-L photoperiod from 12-12 to 10-14 which then largely buffered the negative effect of salt and high temperatures on germination. This capacity of J. acutus and J. maritimus to withstand salty conditions during the germination phase due to the spring photoperiod could be decisive in the ability of both species to colonize saline environments

Distribution de Ludwigia en France : étude des causes de l'expansion récente en Camargue

The current distribution of Ludwigia uruguayensis and L.peploides has been given by a litterature survey and by a national survey. A recent growth of the geographic distribution of these two species, especially along the Atlantic front, has been observed. The commercialization as ornamental species seems to be the main cause of implantation in several regions where the species then multiply by cuttings along the river streams. The results of an experiment show that the L.grandiflora production is extremely influenced by drought and salinity (within the rnge 0 to 10g/l). The current management of the Camargue marshes, which tends to drastically reduce the salinity and summer drought, might explain the recent growth of L.grandiflora in Camargue. / La distribution actuelle de Ludwigia uruguayensis et L. peploides a été établie à partir de l'étude de la littérature et par une enquête nationale. Les deux espèces montrent une extension récente de leur distribution géographique particulièrement le long de la façade atlantique. La commercialisation comme espèce ornementale semble être responsable de l'implantation dans plusieurs régions où les espèces se propagent ensuite principalement par voie végétative le long des cours d'eau. Les résultats d'une expérience montrent que la production de L. grandiflora est très affectée par la sécheresse et la salinité (dans la gamme 0 à 10 g/l.). La gestion actuelle des marais de Camargue qui vise à réduire fortement la salinité et la sécheresse estivale semble pouvoir expliquer l'expansion récente de L. grandiflora en Camargue

Écologie de la restauration : Rencontre des spécialistes de la restauration écologique des écosystème

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