4-(Phenyl­sulfan­yl)benzene-1,2-dicarbonitrile

In the title compound, C14H8N2S, the dicyano-substituted aromatic ring and the phenyl ring attached to the central S atom adopt an angular V-shaped configuration. The dihedral angle between the rings is 103.6°

Automaticity in processing spatial-numerical associations: Evidence from a perceptual orientation judgment task of Arabic digits in frames.

Human adults are faster to respond to small/large numerals with their left/right hand when they judge the parity of numerals, which is known as the SNARC (spatial-numerical association of response codes) effect. It has been proposed that the size of the SNARC effect depends on response latencies. The current study introduced a perceptual orientation task, where participants were asked to judge the orientation of a digit or a frame surrounding the digit. The present study first confirmed the SNARC effect with native Chinese speakers (Experiment 1) using a parity task, and then examined whether the emergence and size of the SNARC effect depended on the response latencies (Experiments 2, 3, and 4) using a perceptual orientation judgment task. Our results suggested that (a) the automatic processing of response-related numerical-spatial information occurred with Chinese-speaking participants in the parity task; (b) the SNARC effect was also found when the task did not require semantic access; and (c) the size of the effect depended on the processing speed of the task-relevant dimension. Finally, we proposed an underlying mechanism to explain the SNARC effect in the perceptual orientation judgment task

Clinical value of miR-23a-3p expression in early diagnosis of diabetic kidney disease

Introduction: The objective was to observe the expression of miR-23a-3p in the serum of patients with type 2 diabetic nephropathy (T2DN) and to explore its clinical significance. Materials and methods: 112 patients with type 2 diabetes were divided into a simple diabetes mellitus (NON) group, T2DN microalbuminuria (MIC) group, and T2DN macroalbuminuria (MAC) group, according to the urinary protein-creatinine ratio (uACR). Clinical data were collected, miR-23a-3p levels in serum were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR), and clinical parameters were measured by an automatic biochemical analyser; the influencing factors of diabetic kidney disease (DKD) and the correlation between miR-23a-3p expression and clinical parameters were analysed. Results: The expression of miR-23a-3p in the serum of the DKD group was lower than that of the normal control (CON) and NON groups. Correlation analysis showed that miR-23a-3p was positively correlated with urinary albumin (Albu), glycosylated haemoglobin (HbA1c), total cholesterol (CHOL), glycated albumin (GA-L), serum creatinine (Scr), fasting blood glucose (GLU), and uric acid (UA), negatively correlated with uACR and high-density lipoprotein cholesterol (HDL-C), but not correlated with urinary creatinine (CREA). The area under the receiver operating characteristic (ROC) curve (AUC) of miR-23a-3p for the diagnosis of DKD was 0.686 [95% confidence interval (CI): 0.599–0.773], with a sensitivity of 64.5% and a specificity of 71.2%; the AUC for differentiating NON from DKD was 0.700 (95% CI: 0.598–0.802), with a sensitivity of 61.8% and a specificity of 77.8%. Multivariate logistic regression analysis showed that serum miR-23a-3plevels were not associated with the development of DKD after adjusting for other levels of influence and were not significant for the differentiation of NON and DKD. Conclusion: Serum miR-23a-3p levels are decreased in T2DN patients, and this change becomes more significant with the severity of the disease, which may be a marker for the early diagnosis and progression of T2DN

Spin polarized proton beam generation from gas-jet targets by intense laser pulses

A method of generating spin polarized proton beams from a gas jet by using a multi-petawatt laser is put forward. With currently available techniques of producing pre-polarized monatomic gases from photodissociated hydrogen halide molecules and petawatt lasers, proton beams with energy ~ 50 MeV and ~ 80 % polarization are proved to be obtained. Two-stage acceleration and spin dynamics of protons are investigated theoretically and by means of fully self-consistent three dimensional particle-in-cell simulations. Our results predict the dependence of the beam polarization on the intensity of the driving laser pulse. Generation of bright energetic polarized proton beams would open a domain of polarization studies with laser driven accelerators, and have potential application to enable effective detection in explorations of quantum chromodynamics

Tetra­methyl 5,5′-[4,5-dicyano-1,2-phenyl­enebis(­oxy)]diisophthalate chloro­form monosolvate

In the title compound, C28H20N2O10·CHCl3, the phen­oxy rings are inclined to the central phenyl ring at dihedral angles of 84.71 (13) and 80.56 (13)°. In the crystal, pairs of weak inter­molecular C—H⋯O hydrogen bonds link mol­ecules related by an inversion center, forming dimers. There are also C—H⋯π inter­actions present

Assessment Accuracy of Standard Point Positioning Enhanced by Observation and Position Domain Filtering Utilizing a Multi-Epoch Least-Squares Integration Method

To enhance the positioning accuracy of standalone GNSS receivers in environments unable to provide precise ephemeris and clock offset, such as undeveloped forest areas that lack network communication and power supply, this study employed the Time Difference Carrier Phase (TDCP) technology to improve the positioning accuracy of Standard Point Positioning (SPP), where the Least-Squares (LS) and the extended Multi-Epoch Least Squares (MELS) method were applied in the position domain filtering for a single GNSS receiver and compare its performance with the existing observation domain filtering method. Firstly, the simulated data sets with various positioning accuracies were used to verify the effectiveness and convergence of the LS filtering methods. The results indicate that the LS filtering method produces a lower root mean square (RMS) error than the original strategy. Secondly, this study uses two kinematic GNSS data sets to evaluate the performance of the observation and position domain filtering, with an emphasis on the MELS method. The numerical experiment results show that the position domain LS filtering method outperforms the other two methods. The open environment experiments result shows that the positioning domain filtering method achieved positioning accuracies of 0.202 m, 0.843 m, and 2.036 m in the E, N, and U directions, respectively, with improvements of 68.0%, 21.6%, and 24.0%, compared to the original algorithm which achieved positioning accuracies of 0.631 m, 1.076 m, and 2.680 m. It also achieved improvements of 24.0%, 4.0%, and 18.3%, respectively, compared to the observation domain filtering method with positioning accuracies of 0.353 m, 0.886 m, and 2.526 m. The forest scenes experiments result shows that the positioning domain filtering method achieved positioning accuracies of 1.308 m, 1.375 m, and 2.133 m in the E, N, and U directions, respectively, with improvements of 42.4%, 36.2%, and 27.6%, compared to original algorithm which achieved positioning accuracies of 1.863 m, 1.873 m, and 2.722 m, and also achieved improvements of 27.0%, 19.4% and 10.6%, respectively, comparing to observation domain filtering method with positioning accuracies of 1.661 m, 1.642 m and 2.359 m. Moreover, the examination of the LS method results based on different epochs reveals that the filtering accuracy increases as more epochs are incorporated into the position domain integration and the enhancement value reaches a few millimeters

Enhanced Expression of Pullulanase in Bacillus subtilis by New Strong Promoters Mined From Transcriptome Data, Both Alone and in Combination

Pullulanase plays an important role as a starch hydrolysis enzyme in the production of bio-fuels and animal feed, and in the food industry. Compared to the methods currently used for pullulanase production, synthesis by Bacillus subtilis would be safer and easier. However, the current yield of pullulanase from B. subtilis is low to meet industrial requirements. Therefore, it is necessary to improve the yield of pullulanase by B. subtilis. In this study, we mined 10 highly active promoters from B. subtilis based on transcriptome and bioinformatic data. Individual promoters and combinations of promoters were used to improve the yield of pullulanase in B. subtilis BS001. Four recombinant strains with new promoters (Phag, PtufA, PsodA, and PfusA) had higher enzyme activity than the control (PamyE). The strain containing PsodA+fusA (163 U/mL) and the strain containing PsodA+fusA+amyE (336 U/mL) had the highest activity among the analyzed dual- and triple-promoter construct stains in shake flask, which were 2.29 and 4.73 times higher than that of the strain with PamyE, respectively. Moreover, the activity of the strain containing PsodA+fusA+amyE showed a maximum activity of 1,555 U/mL, which was 21.9 times higher than that of the flask-grown PamyE strain in a 50-liter fermenter. Our work showed that these four strong promoters mined from transcriptome data and their combinations could reliably increase the yield of pullulanase in quantities suitable for industrial applications

Factors affecting the early establishment of neonatal intestinal flora and its intervention measures

In recent years, it has become evident that early-life intestinal flora plays a pivotal role in determining human health. Consequently, it is imperative to explore the establishment of neonatal intestinal flora and its influencing factors. Early neonatal intestinal flora is influenced by a multitude of factors, including maternal and infant-related factors, as well as external environment. This review summarizes the colonization mechanism of intestinal flora in the early life of newborns and discussed their influence on the establishment of neonatal intestinal flora, taking into account factors such as delivery mode, gestational age and feeding mode. Additionally, this review delves into the natural or artificial reconstruction of intestinal flora colonization defects in infants born via cesarean section and premature infants, with the goal of establishing a theoretical foundation for preventing and treating issues related to neonatal intestinal flora colonization and associated diseases

MAP kinase phosphatase 1 controls innate immune responses and suppresses endotoxic shock

Septic shock is a leading cause of morbidity and mortality. However, genetic factors predisposing to septic shock are not fully understood. Excessive production of proinflammatory cytokines, particularly tumor necrosis factor (TNF)-α, and the resultant severe hypotension play a central role in the pathophysiological process. Mitogen-activated protein (MAP) kinase cascades are crucial in the biosynthesis of proinflammatory cytokines. MAP kinase phosphatase (MKP)-1 is an archetypal member of the dual specificity protein phosphatase family that dephosphorylates MAP kinase. Thus, we hypothesize that knockout of the Mkp-1 gene results in prolonged MAP kinase activation, augmented cytokine production, and increased susceptibility to endotoxic shock. Here, we show that knockout of Mkp-1 substantially sensitizes mice to endotoxic shock induced by lipopolysaccharide (LPS) challenge. We demonstrate that upon LPS challenge, Mkp-1−/− cells exhibit prolonged p38 and c-Jun NH2-terminal kinase activation as well as enhanced TNF-α and interleukin (IL)-6 production compared with wild-type cells. After LPS challenge, Mkp-1 knockout mice produce dramatically more TNF-α, IL-6, and IL-10 than do wild-type mice. Consequently, Mkp-1 knockout mice develop severe hypotension and multiple organ failure, and exhibit a remarkable increase in mortality. Our studies demonstrate that MKP-1 is a pivotal feedback control regulator of the innate immune responses and plays a critical role in suppressing endotoxin shock