Data for "Potato tuber origin and microbial composition determines resistance against soft rot Pectobacteriaceae"

In this study differences in disease incidence between potato seed lots inoculated with soft rot Pectobacteriaceae were assessed in two years. Subsequently the microbial communities in tubers and soils from seed lots with a low and high disease incidence were determined by amplicon sequencing

Natural Infections of Potato Plants Grown from Minitubers with Blackleg-Causing Soft Rot Pectobacteriaceae

Information on the infection incidence of blackleg-causing soft rot Pectobacteriaceae (BL-SRP) in potato crops grown from minitubers (PB1-crop) and the distribution of BL-SRP in individual plants was collected during a two-year survey conducted at five potato growers located in the Netherlands. In the last weeks before haulm destruction, leaves, stems, and tubers of 100 or 200 plants were analyzed separately for the presence of Pectobacterium parmentieri, P. brasiliense, P. atrosepticum, and Dickeya spp. Extracted plant parts enriched for BL-SRP were analyzed with TaqMan assays specific for the detection of blackleg-causing BL-SRP. In 2019, low incidences of P. parmentieri (1–6%) in leaves were found at four growing sites. At one farm, reactions were detected in TaqMan assays for D. zeae and D. chrysanthemi in leaves. In 2020, the crops of two growers were largely free from BL-SRP. At one farm, a high infection incidence (21%) was found for D. fangzhongdai in tubers. The isolated pathogen was able to cause potato blackleg. At two other farms, high infection incidences in tubers were found with P. brasiliense (35–39%) and P. parmentieri (12–19%), whereas the incidence of P. brasiliense in leaves was also high (8%). In conclusion, high infection incidences with BL-SRP in potatoes can be found in a PB1 crop at the end of the growing season. Infections in individual plants were found either in tubers or in leaves. The potential sources of initial infection are discussed

Factors influencing Ralstonia pseudosolanacearum infection incidence and disease development in rose plants

Glasshouse experiments were conducted to study infection and disease development in rockwool-grown rose plants inoculated with Ralstonia pseudosolanacearum. A R. pseudosolanacearum strain isolated from rose plants was more aggressive than strains from anthurium or curcuma. The three rose cultivars tested, Avalanche, Red Naomi, and Armando, differed in susceptibility. At 20°C, the rose strain caused hardly any symptoms over a 6-week period, whereas at 28°C typical wilt symptoms were observed within 2 weeks after stem inoculation of Armando, the most susceptible cultivar. Inoculating roots with the rose strain resulted only in weak atypical symptoms. Nevertheless, inoculating roots of cv. Armando at a relatively low inoculum dose of 104 cfu/ml led to high densities in the base of stems in one out of two experiments. R. pseudosolanacearum occasionally spread from stem inoculated plants with symptoms in rockwool slabs. This limited spread resulted in a low infection incidence, and only of plants directly adjacent to the plants with symptoms

Mapping tracks of Xanthomonas campestris pv. campestris resulting in Brassica seed infections

In this report research is described, conducted by Wageningen Plant Research (Wageningen Universityand Research) in collaboration with the seed companies Bejo Zaden b.v., Syngenta Seeds b.v., ENZA Zaden Research and Development b.v. and Rijk Zwaan Zaadteelt and Zaadhandel b.v. within the frame of public private partnership project entitled ‘Routes van Xanthomonas campestris pv.campestris die resulteren in zaadinfecties in Brassica in kaart brengen’ (Mapping tracks ofXanthomonas campestris pv. campestris resulting in Brassica seed infections).The project in Netherlands was granted by the Topsector ‘Horticulture and propagation material’ of the Dutch Ministry of Economic Affairs. It was conducted under grant number KV1505 095

Monascus ruber as cell factory for lactic acid production at low pH

A Monascus ruber strain was isolated that was able to grow on mineral medium at high sugar concentrations and 175 g/l lactic acid at pH 2.8. Its genome and transcriptomes were sequenced and annotated. Genes encoding lactate dehydrogenase (LDH) were introduced to accomplish lactic acid production and two genes encoding pyruvate decarboxylase (PDC) were knocked out to subdue ethanol formation. The strain preferred lactic acid to glucose as carbon source, which hampered glucose consumption and therefore also lactic acid production. Lactic acid consumption was stopped by knocking out 4 cytochrome-dependent LDH (CLDH) genes, and evolutionary engineering was used to increase the glucose consumption rate. Application of this strain in a fed-batch fermentation resulted in a maximum lactic acid titer of 190 g/l at pH 3.8 and 129 g/l at pH 2.8, respectively 1.7 and 2.2 times higher than reported in literature before. Yield and productivity were on par with the best strains described in literature for lactic acid production at low pH

Desde la niñez a la vejez : nuevos desafíos para la comprensión de la sociología de las edades

En este libro nos planteamos el siguiente objetivo: abordar las problemáticas que tuvieran como eje central las distintas etapas del curso de vida desde una perspectiva longitudinal. De este modo, entendimos el envejecimiento como un proceso que ocurre a lo largo de toda la vida y que ha consolidado campos de interés sociológicos diferenciales. La niñez, la juventud y la vejez aparecen as. como construcciones sociales históricamente constituidas, que institucionalizan muchas veces el actuar de los sujetos. Es as. que, a partir de la estratificación social de la "edad", muchas veces niños, jóvenes y viejos son sujetos de debate de inequidad y desigualdad social, tanto desde la agenda social como política