1,863 research outputs found
Investigation into reversed phase chromatography peptide separation systems part II : an evaluation of the robustness of a protocol for column characterisation
The robustness of the Peptide Reversed Phase Chromatography (RPC) Column Characterisation Protocol was evaluated using reduced factorial design, to ascertain the degree of control required for parameters including temperature, flow rate, dwell volume, a systematic shift in the gradient, amount of formic acid in the aqueous and organic, pH of the ammonium formate and amount of acetonitrile (%MeCN) in the strong solvent, where a loss of MeCN resulted in an unacceptable variation. Mitigations have been introduced to ensure the integrity of the data to allow RPC columns to be characterised using peptides as probes, with the definitive protocol described. In addition, the instrument and column batch to batch variability were assessed with good reproducibility
Improved Fan Control
Cooling large computer enclosures is an increasingly difficult task, usually requiring multiple fans and/or multiple rotors per fan. A technique is disclosed that reduces stress on a power distribution system for multiple fans by delaying individual fan control signals at the final distribution point near the fans
The Art of Clinical Supervision: Pastoral Counseling (New York and Mahwah, NJ: Paulist Press, 1987)
Reviewed by Peter Yuichi Clark
A 3,500-year tree-ring record of annual precipitation on the northeastern Tibetan Plateau
An annually resolved and absolutely dated ring-width chronology spanning 4,500 y has been constructed using subfossil, archaeological, and living-tree juniper samples from the northeastern Tibetan Plateau. The chronology represents changing mean annual precipitation and is most reliable after 1500 B.C. Reconstructed precipitation for this period displays a trend toward more moist conditions: the last 10-, 25-, and 50-y periods all appear to be the wettest in at least three and a half millennia. Notable historical dry periods occurred in the 4th century BCE and in the second half of the 15th century CE. The driest individual year reconstructed (since 1500 B.C.) is 1048 B.C., whereas the wettest is 2010. Precipitation variability in this region appears not to be associated with inferred changes in Asian monsoon intensity during recent millennia. The chronology displays a statistical association with the multidecadal and longer-term variability of reconstructed mean Northern Hemisphere temperatures over the last two millennia. This suggests that any further large-scale warming might be associated with even greater moisture supply in this region
Investigation into reversed phase chromatography peptide separation systems part I : development of a protocol for column characterisation
A protocol was defined which utilised peptides as probes for the characterisation of reversed phase chromatography peptide separation systems. These peptide probes successfully distinguished between differing stationary phases through the probe's hydrophobic, electrostatic, hydrogen bonding and aromatic interactions with the stationary phase, in addition, to more subtle interactions such as the phase's ability to separate racemic or isomeric probes. The dominating forces responsible for the chromatographic selectivity of peptides appear to be hydrophobic as well as electrostatic and polar in nature. This highlights the need for other types of stationary phase ligands with possibly mixed mode functionalities / electrostatic / polar interactions for peptide separations rather than the hydrophobic ligands which dominate small molecule separations. Selectivity differences are observed between phases, but it appears that it is the accessibility differences between these phases which play a crucial role in peptide separations i.e. accessibility to silanols, the hydrophobic acetonitrile / ligand layer or a thin adsorbed water layer on the silica surface
Exotic torus manifolds and equivariant smooth structures on quasitoric manifolds
In 2006 Masuda and Suh asked if two compact non-singular toric varieties
having isomorphic cohomology rings are homeomorphic. In the first part of this
paper we discuss this question for topological generalizations of toric
varieties, so-called torus manifolds. For example we show that there are
homotopy equivalent torus manifolds which are not homeomorphic. Moreover, we
characterize those groups which appear as the fundamental groups of locally
standard torus manifolds.
In the second part we give a classification of quasitoric manifolds and
certain six-dimensional torus manifolds up to equivariant diffeomorphism.
In the third part we enumerate the number of conjugacy classes of tori in the
diffeomorphism group of torus manifolds. For torus manifolds of dimension
greater than six there are always infinitely many conjugacy classes. We give
examples which show that this does not hold for six-dimensional torus
manifolds.Comment: 21 pages, 2 figures, results about quasitoric manifolds adde
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