Antioxidant potential assessment of phenolic and flavonoid rich fractions of <i>Clematis orientalis</i> and <i>Clematis ispahanica</i> (Ranunculaceae)

<p>The antioxidant activities of crude extract fractions using Hexane, Chloroform, Ethyl Acetate, Butanol and Water of <i>Clematis orientalis</i> and <i>Clematis ispahanica</i> were investigated. 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay and the ferric reducing/antioxidant potential (FRAP) were used to evaluate the antioxidant capacity. The total phenolics were found to be 4.37–9.38 and 1.32–11.37 mg gallic acid equivalents (GAE)/g in different fractions for <i>C. orientalis</i> and <i>C. ispahanica</i>, respectively. The ethyl acetate fraction of <i>C. orientalis</i> and chloroform fraction of <i>C. ispahanica</i> showed the highest DPPH and FRAP activities at a concentration of 300 μg/mL. The predominant phenolic compounds identified by HPLC in <i>C. orientalis</i> were Resorcinol (603.5 μg/g DW) in chloroform fraction and Ellagic acid (811.7 μg/g DW) in chloroform fraction of <i>C. ispahanica</i>.</p