An Unconventional Glutamatergic Circuit in the Retina Formed by vGluT3 Amacrine Cells

SummaryIn the vertebrate retina, glutamate is traditionally thought to be released only by photoreceptors and bipolar cells to transmit visual signals radially along parallel ON and OFF channels. Lateral interactions in the inner retina are mediated by amacrine cells, which are thought to be inhibitory neurons. Here, we report calcium-dependent glutamate release from vGluT3-expressing amacrine cells (GACs) in the mouse retina. GACs provide an excitatory glutamatergic input to ON-OFF and ON direction-selective ganglion cells (DSGCs) and a subpopulation of W3 ganglion cells, but not to starburst amacrine cells. GACs receive excitatory inputs from both ON and OFF channels, generate ON-OFF light responses with a medium-center, wide-surround receptive field structure, and directly regulate ganglion cell activity. The results reveal a functional glutamatergic circuit that mediates noncanonical excitatory interactions in the retina and probably plays a role in generating ON-OFF responses, crossover excitation, and lateral excitation

Airframe-Propulsion Integration Design and Optimization

Airframe-propulsion integration design is one of the key technologies of the hypersonic vehicle. With the development of hypersonic vehicle design method, CFD technology, and optimization method, it is possible to improve the conceptual design of airframe-propulsion integration both in accuracy and efficiency. In this chapter, design methods of waverider airframes and propulsion systems, including inlets, nozzles, isolators, and combustors, are reviewed and discussed in the light of CFD analyses. Thereafter, the Busemann inlet, a three-dimensional flow-stream traced nozzle, and a circular combustor together with a cone-derived waverider are chosen to demonstrate the airframe-propulsion integration design. The propulsion system is optimized according to the overall performance, and then the component such as the nozzle is optimized to obtain a better conceptual configuration

Spatiotemporal features of the soil moisture across Northwest China using remote sensing data, reanalysis data, and global hydrological model

Soil moisture is an important factor affecting the change of land surface hydrological processes and the distribution of material and energy exchanges between the land and atmosphere and vegetation’s temporal and spatial distributions, especially in arid and semi-arid regions. This paper focuses on soil moisture features across Northwest China, the core region of the Silk Road Economic Belt. Six soil moisture datasets from the period 1981–2020 were employed, which included ERA5 (the European Centre for Medium-Range Weather Forecasts Atmospheric Reanalysis 5), ESA-CCI (European Space Agency Climate Change Initiative), GLDAS (Global Land Data Assimilation System), MERRA-2 (The Modern-Era Retrospective Analysis for Research and Applications, Version 2), RSSSM (A Remote Sensing-based global 10-day resolution Surface Soil Moisture dataset), and SSM-Feng (Regional multimodal fusion of surface soil moisture data in China). The temporal and spatial variation of the linear trend and abrupt change characteristics at seasonal and annual scale were explored. The results are as follows: 1) ESA-CCI, GLDAS, and MERRA-2 showed a slow increase in annual soil moisture tendency at a rate of less than 0.001 m3/m3/year, while ERA5 and SSM-Feng showed a significant decreasing linear trend at a rate of 1.31 × 10−4 m3/m3/year and 1.01 × 10−4 m3/m3/year (p < 0.05), respectively. 2) In autumn and winter, only GLDAS and MERRA-2 showed significant increasing trends. In the growing season (i.e., from April to October), the soil moisture of ESA-CCI, GLDAS, and MERRA-2 significantly increased at the rates of 3.29 × 10−4 m3/m3/year, 3.30 × 10−4 m3/m3/year, and 6.64 × 10−4 m3/m3/year (p < 0.05), respectively. 3) ERA5 and ESA-CCI have frequent abrupt changes in 1984, 1987, and 2006 for ERA5, 2010–2012 and 2019–2020 for ESA-CCI. 4) In terms of spatial variations, most datasets show that soil moisture has increased across most regions. The ERA5, ESA-CCI, GLDAS, MERRA-2, and SSM-Feng datasets show decreased soil moisture in the Tarim Basin. The conclusions of this study deepen the understanding of temporal and spatial variation in soil moisture in arid areas of Northwest China. Through these conclusions, a certain theoretical basis can be provided for the complex water cycle process in the arid region

Comparative transcriptome analysis of genes involved in paradormant bud release response in ‘Summer Black’ grape

Grapevines possess a hierarchy of buds, and the fruitful winter bud forms the foundation of the two-crop-a-year cultivation system, yielding biannual harvests. Throughout its developmental stages, the winter bud sequentially undergoes paradormancy, endodormancy, and ecodormancy to ensure survival in challenging environmental conditions. Releasing the endodormancy of winter bud results in the first crop yield, while breaking the paradormancy of winter bud allows for the second crop harvest. Hydrogen cyanamide serves as an agent to break endodormancy, which counteracting the inhibitory effects of ABA, while H2O2 and ethylene function as signaling molecules in the process of endodormancy release. In the context of breaking paradormancy, common agronomic practices include short pruning and hydrogen cyanamide treatment. However, the mechanism of hydrogen cyanamide contributes to this process remains unknown. This study confirms that hydrogen cyanamide treatment significantly improved both the speed and uniformity of bud sprouting, while short pruning proved to be an effective method for releasing paradormancy until August. This observation highlights the role of apical dominance as a primary inhibitory factor in suppressing the sprouting of paradormant winter bud. Comparative transcriptome analysis revealed that the sixth node winter bud convert to apical tissue following short pruning and established a polar auxin transport canal through the upregulated expression of VvPIN3 and VvTIR1. Moreover, short pruning induced the generation of reactive oxygen species, and wounding, ethylene, and H2O2 collectively acted as stimulating signals and amplified effects through the MAPK cascade. In contrast, hydrogen cyanamide treatment directly disrupted mitochondrial function, resulting in ROS production and an extended efficacy of the growth hormone signaling pathway induction

Perioperative nutritional risk and its influencing factors in patients with oral cancer: a longitudinal study

IntroductionWe aimed to investigate the nutritional risk status and dynamic changes in patients with perioperative oral cancer at different stages and analyze the factors influencing nutritional risk and the correlation among body mass index, nutrition-related symptoms, and nutritional risk.MethodsIn total, 198 patients with oral cancer who were hospitalized in the Head & Neck Surgery Departments of a tertiary cancer hospital in Hunan Province, China, from May 2020 to January 2021, were selected as participants. The Nutritional Risk Screening 2002 scale and Head and Neck Patient Symptom Checklist were used to assess patients on admission day, 7 days post-surgery, and 1 month post-discharge. Multivariate analysis of variance, paired t-test, and generalized estimating equation were used to analyze the trajectory and influencing factors of nutritional risk in patients with perioperative oral cancer. Spearman’s correlation analysis was used to explore the correlation among body mass index, symptoms, and nutritional risk.ResultsThe nutritional risk scores of patients with oral cancer at the three time points were 2.30 ± 0.84, 3.21 ± 0.94, and 2.11 ± 0.84, respectively, and the differences were significant (p < 0.05). The incidences of nutritional risk were 30.3, 52.5, and 37.9%, respectively. The factors influencing nutritional risk included education level, smoking status, clinical stage, flap repair, and tracheotomy (β = −0.326, 0.386, 0.387, 0.336, and 0.240, respectively, p < 0.05). Nutritional risk was negatively correlated with body mass index (rs = −0.455, p < 0.01) and positively correlated with pain, loss of appetite, sore mouth, bothersome smells, swallowing difficulty, taste changes, depression, chewing difficulty, thick saliva, and anxiety (rs = 0.252, 0.179, 0.269, 0.155, 0.252, 0.212, 0.244, 0.384, 0.260, and 0.157, respectively, p < 0.05).ConclusionThe incidence of nutritional risk in patients with perioperative oral cancer was high, and the trajectory of nutritional risk changed over time. Strengthening the nutritional monitoring and management of postoperative patients or those with low education level, advanced-stage cancer, flap repair, tracheotomy, and low body mass index; strengthening tobacco control management; and controlling nutrition-related discomfort symptoms in perioperative oral cancer patients are necessary

Role of Alkaline-Earth Metal-Catalyst: A Theoretical Study of Pyridines Hydroboration

Density functional theory (DFT) calculations have been performed to investigate the mechanism of alkaline-earth-metal-catalyzed hydroboration of pyridines with borane. In this reaction, the active catalytic species is considered to be an alkaline earth metal hydride complex when the corresponding alkaline earth metal is used as the catalyst. The theoretical results reveal that initiation of the catalytic cycle is hydride transfer to generate a magnesium hydride complex when β-diimine alkylmagnesium is used as a pre-catalyst. The magnesium hydride complex can undergo coordination of the pyridine reactant followed by hydride transfer to form a dearomatized magnesium pyridine intermediate. Coordination of borane and hydride transfer from borohydride to magnesium then give the hydroboration product and regenerate the active magnesium hydride catalyst. The rate-determining step of the catalytic cycle is hydride transfer to pyridine with a free energy barrier of 29.7 kcal/mol. Other alkaline earth metal complexes, including calcium and strontium complexes, were also considered. The DFT calculations show that the corresponding activation free energies for the rate-determining step of this reaction with calcium and strontium catalysts are much lower than with the magnesium catalyst. Therefore, calcium and strontium complexes can be used as the catalyst for the reaction, which could allow mild reaction conditions

An Improved Detection of Circulating Tumor DNA in Extracellular Vesicles-Depleted Plasma

Circulating tumor DNA (ctDNA) in plasma has been used as a biomarker for cancer detection and outcome prediction. In this study, we collected the five precipitates (fractions 1–5) and leftover supernatant plasma component (fraction 6) by a sequential centrifugation in plasma samples from nine small cell lung cancer (SCLC) patients. The fractions 3, 5 and 6 were large vesicles, exosomes and extracellular vesicles (EVs)-depleted plasma, respectively. Fragment size analysis using DNAs from these fractions showed dramatical differences from a peak of 7–10 kb in fraction 1 to 140–160 bp in fraction 6. To determine ctDNA content, we performed whole genome sequencing and applied copy number-based algorithm to calculate ctDNA percentage. This analysis showed the highest ctDNA content in EV-depleted plasma (average = 27.22%), followed by exosomes (average = 22.09%) and large vesicles (average = 19.70%). Comparatively, whole plasma, which has been used in most ctDNA studies, showed an average of 23.84% ctDNA content in the same group of patients. To further demonstrate higher ctDNA content in fraction 6, we performed mutational analysis in the plasma samples from 22 non-small cell lung cancer (NSCLC) patients with known EGFR mutations. This analysis confirmed higher mutation detection rates in fraction 6 (14/22) than whole plasma (10/22). This study provides a new insight into potential application of using fractionated plasma for an improved ctDNA detection