Topical effectiveness of different concentrations of nanosilver solution on Leishmania major lesions in Balb/c mice

Background & objectives: Cutaneous leishmaniasis is an infection caused by protozoan genus Leishmania. Althoughglucantime is commonly used for the treatment of leishmaniasis, it has some side effects including increased liverenzymes and electrocardiogram changes. In addition, the drug is expensive, the injection is painful, and researchshows that resistance of parasite to glucantime is growing in different parts of the world. Therefore, scientists arepaying more attention to develop new drugs such as nanosilver solution. The present study is an attempt toevaluate the in vivo topical effects of different concentrations of nanosilver solution in the treatment of leishmaniasislesions.Methods: In all, 90 female Balb/c mice aged 6–8 wk were infected with 2×106 viable stationary-phase promastigotesin the base of tail. Different concentrations (60, 80, 120, 130 and 2000 ppm) nanosilver solution were used in thepresent study to test the efficacy in the treatment of lesions. Clinical control of the infection trends was conductedweekly for 5 wk by measuring lesion diameter with standard Kulis-Vernieh. Data were analyzed by paired t-test,analysis of variance (ANOVA), and Tukey test.Results: Mean lesion diameter pre- and post-treatment did not significantly differ between different treatmentgroups (p >0.05). Likewise, a significant difference in splenic parasite load was also not observed between differenttreatment groups.Interpretation & conclusion: Based on our results, different concentrations of nanosilver are ineffective in reducingmean sizes of lesions

Specific immune responses induced by multi-epitope DNA derived from Mycobacterium tuberculosis DosR antigens

One third of the world population are latently infected with Mycobacterium tuberculosis and are at the risk of reactivation of tuberculosis (TB). The most effective strategy for control of TB worldwide is the development of a vaccine that inhibits progression of latent TB to active infection. In this study, two optimized constructs consisting of multi-epitopes DNA derived from three latency antigens Rv2029c, Rv2031c, and Rv2627c fused with or without light chain 3 (LC3) are synthetized. The immunogenicity effectiveness of two DNA constructs was evaluated in the mouse model. LC3-fused multi-epitope DNA construct induced strong specific Th1 immune responses with high increase in IFN-γ+ CD4+ and IL-2+ CD4+ T cell populations (both with p + IL-2+ CD4+ T cell population (p + CD8+ T cell population (p + and CD8+ T cell populations. The results indicated that LC3-fused multi-epitope DNA construct has a potential to be investigated for future development of a new TB vaccine

Electron-based crystalline undulator

We discuss the features of a crystalline undulator of the novel type based on the effect of a planar channeling of ultra-relativistic electrons in a periodically bent crystals. It is demonstrated that an electron-based undulator is feasible in the tens of GeV range of the beam energies, which is noticeably higher than the energy interval allowed in a positron-based undulator. Numerical analysis of the main parameters of the undulator as well as the characteristics of the emitted undulator radiation is carried out for 20 and 50 GeV electrons channeling in diamond and silicon crystals along the (111) crystallographic planes.Comment: 16 pages, 8 figures, Latex, IOP styl

Fare Free Public Transportation: A Full-Scale Real-World Experiment in Alexandria (VA)

69A43551747123The Fare Free Public Transportation (FFPT) concept is a common part of the agenda among transit agencies and state and federal policy makers. The subject is particularly important in the post-pandemic period, as transit use is slowly recovering but has not yet reached pre-pandemic ridership and market share. FFPT has been implemented in Europe and to a certain degree in the USA; however, there are very few studies that have effectively collected data and evaluated the consequences with respect to its implementation. This study monitored a full-scale, real-world FFPT plan implemented in Alexandria, VA in the Fall of 2021, separating respondents into treatment and control groups. Descriptive statistics indicated minimal disparity between the treatment and control groups across most socio-demographic variables. Notably, residents of Alexandria exhibit a higher propensity to use buses compared to the control group, both prior to and post-policy implementation. Regarding awareness of the policy, a majority of respondents were uninformed, while the policy's impact is more pronounced among those who were aware. Around 32% of respondents increased their bus usage following FFPT implementation, with approximately 80% of this subset utilizing buses more frequently than before. This policy evaluation is relevant not only to Alexandria, but to many stakeholders across the country that are considering similar policies in other cities

Relationships between self-discrepancies, emotions and depression in males and females

Self-discrepancy theory postulates that individuals who experience selfdiscrepancies feel uncomfortable, or disturbed, and may manifest emotional problems such as depression, dejection or agitation. This study examined selfdiscrepancies in relation to self-reported dejection, agitation, dependent depression and self-critical depression. The study also examined sex differences in the relationships between self-discrepancies and these different emotional states. Undergraduate students (96 males and 119 females) completed the Selves Questionnaire to measure self-discrepancies, the Emotions Questionnaire to measure dejection and agitation affects, and the Depressive Experiences Questionnaire to measure the dependent and self-critical forms of depression. Four different self-discrepancies were computed from the Selves Questionnaire; actual/ideal/own(AIOW), actual/ought/own(AOOW), actual/ideal/other(AIOT), and actual/ought/other (AOOT). The results using partial correlations controlling for the remaining self-discrepancies and the other dependent variable (either dejection or agitation and dependency or self-criticism) showed that only AOOT discrepancies predicted agitation in women, and none of selfdiscrepancies predicted dejection or agitation in men. The dependent and selfcritical types of depression correlated with some types of self-discrepancies differently in men and women. These differences suggest that the determinants of these negative affects may, to a limited degree, be different in men and women

How will mandibular third molar surgery affect mandibular second molar periodontal parameters?

Background: Several conflicting findings have been published in the previous literature regarding the effects of impacted third molar surgery on the periodontal parameters of the adjacent second molar; some authors have shown improvement of periodontal health distal to the adjacent second molar, whilst others have demonstrated loss of attachment level (AL) and reduction of alveolar bone height. The purpose of this study was to evaluate the changes in periodontal health parameters distal to the adjacent second molar following extraction of an impacted third molar. Materials and Methods: Out of 50 patients participated in the study, 42 patients completed the study. The mean age of the sample was 20.9 (range, 18-25) years. All teeth were mesioangular impacted mandibular third molars categorized at C1 class based on the Pell and Gregory classification. All surgeries were performed by one surgeon and the same surgeon recorded the pre-operative and post-operative measurements of probing depth (PD) and AL on the distobuccal aspect of the second molars. Data analysis were carried out with the SPSS software (version 19), using the paired-samples t-test and one sample t-test. Results: Surgical extraction of impacted mandibular third molar resulted in a significant increase of PD on the distobuccal aspect of the second molars, whereas AL was decreased significantly after surgery (P < 0.05). Conclusion: Unlike plenty of researches that have shown improvement of periodontal parameters of the second molar after extraction of impacted third molar, our study showed a significant increase in PD at the distal aspect of the second molar. Further follow-up on clinical and radiological parameters are required for more profound understanding of the long-term effects of third molar extraction on the periodontal parameters of the adjacent second molar

Audit de la morbidité des cytoréductions chirurgicales dans les cancers avancés de l'ovaire (étude multicentrique rétrospective dans 6 Centres français)

LILLE2-BU Santé-Recherche (593502101) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Investigations on the cardinal temperatures for germination of Plantago ovata and Plantago psyllium

A laboratory study was conducted to investigate the effect of different temperatures on seed germination of two species of Plantago namely P. ovata and P. psyllium in a completely randomized design with four replications. Various constant temperature (5, 7, 10, 15, 20, 25, 30 and 35 ◦C) were considered. According to the results, the effect of temperature on rate and percentage of seed germination in both species was significant. The highest germination rates were in 20 and 25 ◦C for P. ovata and P. psyllium, respectively. Maximum seed germination occurred for P. ovata (100%) and P. psyllium (99%) in 15 and 25 ◦C , respectively. Optimum thermal range for seed germination of P. ovata was (10-20◦C) and for P. psyllium was (15-25◦C). By increasing or decreasing temperature beyond the optimal thermal range, percentage of seed germination was decreased significantly. Based on linear regression between germination rate and temperature in both species, the cardinal temperatures: minimum(Tmin), optimum(To) and maximum (Tmax) were determined which were: 4.4, 19.0 and 25.5 ◦C for P.ovata and 9.4, 28.8 and 35.0◦C for P. psyllium, respectively

Organic cultivation of Plantago ovata and Plantago psyllium in response to water stress

In order to investigate the response of two species of P. ovata and P. psyllium to water deficit and manure, an experiment was conducted at the Agricultural Research Station, Ferdowsi University of Mashhad, in April 2002. For this purpose a split-factorial experiment based on completely randomized block design with three replications was used. Three irrigation intervals (every 10, 20 and 30 days) allocated in the main plots and three levels of animal manure (5, 10 and 15 tons/ha) and two species of plantago in factorial arrangement were as sub plots. Criteria such as plant height, spike length, number of spikes per plant, number of seeds per spike, 1000-seed weight, straw and seed yield were measured accordingly. Three quality characters namely amount of mucilage, swelling factor and swelling rate per gram mucilage were also measured. The results indicated that spike length and seed yield were significantly affected by irrigation intervals. The highest seed yield in P. ovata and P. psyllium was obtained in irrigation intervals of 20 and 10 days, respectively. There was no significant effect on another criteria by irrigation intervals. Effect of animal manure was only significant on seed yield. Seed yield was higher with application of 5 tons/ha and 15 tons/ha in P. ovata and P. psyllium, respectively. There was no significant correlation between the amount of mucilage, swelling factor and 1000-seed weight. However, the highest amount of mucilage were obtained in irrigation intervals of 30 and 10 days in P. ovata and P. psyllium respectively, maximum swelling factor with irrigation in 30 days interval and the highest swelling rate per gram mucilage in P. ovata and P. psyllium by irrigation intervals of 20 and 10 days, respectively. In water deficit condition and low-input agricultural systems it seems that P. ovata is more resistant species compared with P. psyllium