Research Notes : United States : Heterosis performance and combining ability in soybeans

Before hybrid soybeans can become a reality, two requirements should be satisfied: (i) an economical large-scale method of producing hybrid seed must be found, and (ii) heterosis for yield must exist. With the findings of genetic male sterility in soybeans (Brim and Young, 1971) interest has devel-oped in the potential productivity of hybrid soybeans. A method for produc-ing experimental quantities of hybrid soybean seed using genetic male sterility and green seed embryo was suggested by Burton and Carter (1983)

Influence of Nitrogen Rate and Sampling Date on Soil Analysis Values of Highbush Blueberries

Three studies were conducted on nitrogen (N) fertilization effects on soil of highbush blueberries in Arkansas. Objectives included: 1) determination of varying N rate effects on the standard soil analysis variables, 2) determination of date of soil sampling effects on soil analyses, 3) evaluation of the interactions of N rate and time of sampling, and 4) determination of changes in soil content over years. This research was done in two of the more important highbush blueberry production areas in Arkansas: the Arkansas River Valley where blueberries are grown on sandy loam soils, and northwest Arkansas where production is more common on silt loam soils. This information provides greater insight into the soil dynamics of highbush blueberry plantings in Arkansas and can be used to increase precision of fertility and other soil property recommendations provided to growers

Research Notes : United States : The effect of the narrow-leaf gene in a segregating population

A single recessive gene designated Jn by Bernard and Weiss (1972) controls the inheritance of the lanceolate trifoliolate in soybean. They state that the narrow-leaf condition is associated with a high number of four-seeded pods, which they attributed to a pleiotropic effect of the Jn gene. No yield differences have been found between narrow and normal leaf types using isolines

Upper White River BMP Implementation Project (NPS Final Report)

The project objective was to monitor agricultural best management practices implemented to minimize sediment, nutrient, and bacterial impact on water quality of the Upper White River watershed. The project targeted the primary agricultural causes of non-point source nutrient and bacterial pollution in three sub-basins of the White River in the Beaver Lake Watershed. Areas with high animal densities targeted high source areas. High source areas were treated with best management practices (BMP) in an effort to reduce the impact to the White River and Beaver Lake. The predominant BMP implemented was waste management, a component of the farm nutrient management plan

Hybrid forage sorghum performance trials in Oklahoma, 1989

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Negative regulation of syntaxin4/SNAP-23/VAMP2-mediated membrane fusion by Munc18c <i>In Vitro</i>

Background: Translocation of the facilitative glucose transporter GLUT4 from an intracellular store to the plasma membrane is responsible for the increased rate of glucose transport into fat and muscle cells in response to insulin. This represents a specialised form of regulated membrane trafficking. Intracellular membrane traffic is subject to multiple levels of regulation by conserved families of proteins in all eukaryotic cells. Notably, all intracellular fusion events require SNARE proteins and Sec1p/Munc18 family members. Fusion of GLUT4-containing vesicles with the plasma membrane of insulin-sensitive cells involves the SM protein Munc18c, and is regulated by the formation of syntaxin 4/SNAP23/VAMP2 SNARE complexes. Methodology/Principal Findings Here we have used biochemical approaches to characterise the interaction(s) of Munc18c with its cognate SNARE proteins and to examine the role of Munc18c in regulating liposome fusion catalysed by syntaxin 4/SNAP23/VAMP2 SNARE complex formation. We demonstrate that Munc18c makes contacts with both t- and v-SNARE proteins of this complex, and directly inhibits bilayer fusion mediated by the syntaxin 4/SNAP23/VAMP2 SNARE complex. Conclusion/Significance Our reductionist approach has enabled us to ascertain a direct inhibitory role for Munc18c in regulating membrane fusion mediated by syntaxin 4/SNAP23/VAMP2 SNARE complex formation. It is important to note that two different SM proteins have recently been shown to stimulate liposome fusion mediated by their cognate SNARE complexes. Given the structural similarities between SM proteins, it seems unlikely that different members of this family perform opposing regulatory functions. Hence, our findings indicate that Munc18c requires a further level of regulation in order to stimulate SNARE-mediated membrane fusion

Chlorpromazine reduces avoidance performance deficit in rats with dorsomedial thalamic lesions

Rats with bilateral lesions of the dorsomedial area of the thalamus were shown to have a significant performance deficit on the acquisition of a one-way active shock-avoidance task relative to sham-operated controls. Administration of chlorpromazine prior to testing was shown to reduce this deficit among the DMT-lesioned animals

The binding of Varp to VAMP7 traps VAMP7 in a closed, fusogenically inactive conformation.

SNAREs provide energy and specificity to membrane fusion events. Fusogenic trans-SNARE complexes are assembled from glutamine-contributing SNAREs (Q-SNAREs) embedded in one membrane and an arginine-contributing SNARE (R-SNARE) embedded in the other. Regulation of membrane fusion events is crucial for intracellular trafficking. We identify the endosomal protein Varp as an R-SNARE-binding regulator of SNARE complex formation. Varp colocalizes with and binds to VAMP7, an R-SNARE that is involved in both endocytic and secretory pathways. We present the structure of the second ankyrin repeat domain of mammalian Varp in complex with the cytosolic portion of VAMP7. The VAMP7-SNARE motif is trapped between Varp and the VAMP7 longin domain, and hence Varp kinetically inhibits the ability of VAMP7 to form SNARE complexes. This inhibition will be increased when Varp can also bind to other proteins present on the same membrane as VAMP7, such as Rab32-GTP

Intracellular Bacteria Encode Inhibitory SNARE-Like Proteins

Pathogens use diverse molecular machines to penetrate host cells and manipulate intracellular vesicular trafficking. Viruses employ glycoproteins, functionally and structurally similar to the SNARE proteins, to induce eukaryotic membrane fusion. Intracellular pathogens, on the other hand, need to block fusion of their infectious phagosomes with various endocytic compartments to escape from the degradative pathway. The molecular details concerning the mechanisms underlying this process are lacking. Using both an in vitro liposome fusion assay and a cellular assay, we showed that SNARE-like bacterial proteins block membrane fusion in eukaryotic cells by directly inhibiting SNARE-mediated membrane fusion. More specifically, we showed that IncA and IcmG/DotF, two SNARE-like proteins respectively expressed by Chlamydia and Legionella, inhibit the endocytic SNARE machinery. Furthermore, we identified that the SNARE-like motif present in these bacterial proteins encodes the inhibitory function. This finding suggests that SNARE-like motifs are capable of specifically manipulating membrane fusion in a wide variety of biological environments. Ultimately, this motif may have been selected during evolution because it is an efficient structural motif for modifying eukaryotic membrane fusion and thus contribute to pathogen survival