Feasibility of Podcasts and City by City Analysis for Upside.FM

The objective of the project is to conduct a feasibility study for Upside, with regard to their underlying business model. We then hope to make recommendations for other cities and complementary products that can fit under the Upside umbrella

Calcium negatively regulates secretion from dense granules in Toxoplasma gondii.

Apicomplexan parasites including Toxoplasma gondii and Plasmodium spp. manufacture a complex arsenal of secreted proteins used to interact with and manipulate their host environment. These proteins are organised into three principle exocytotic compartment types according to their functions: micronemes for extracellular attachment and motility, rhoptries for host cell penetration, and dense granules for subsequent manipulation of the host intracellular environment. The order and timing of these events during the parasite's invasion cycle dictates when exocytosis from each compartment occurs. Tight control of compartment secretion is, therefore, an integral part of apicomplexan biology. Control of microneme exocytosis is best understood, where cytosolic intermediate molecular messengers cGMP and Ca2+ act as positive signals. The mechanisms for controlling secretion from rhoptries and dense granules, however, are virtually unknown. Here, we present evidence that dense granule exocytosis is negatively regulated by cytosolic Ca2+ , and we show that this Ca2+ -mediated response is contingent on the function of calcium-dependent protein kinases TgCDPK1 and TgCDPK3. Reciprocal control of micronemes and dense granules provides an elegant solution to the mutually exclusive functions of these exocytotic compartments in parasite invasion cycles and further demonstrates the central role that Ca2+ signalling plays in the invasion biology of apicomplexan parasites.Medical Research Council Australian Research Counci

Aggregations of a Sessile Ctenophore, Coeloplana sp., on Indo-West Pacific Gorgonians

We document the benthic ctenophores Coeloplana sp. and Vallicula multiformis from Oman, extending their geographic range. A new Coeloplana species was found forming aggregations on gorgonians of two octocoral host genera, Melithaea and Euplexaura, representing associations previously unknown to occur in the Indo-West Pacific region. Our findings also illustrate the concurrent presence of the ectocommensal ophiuroid Ophiothela mirabilis, which adversely affects other Coeloplana species in the tropical West Atlantic, where it is considered invasive. This exploration contributes to our understanding of the biogeography, species distribution, and ectosymbiotic associations of these genera, setting the stage for a comprehensive species description and in-depth analysis of host relationships in future studies

Magnesium(II)-ATP Complexes in 1-Ethyl-3-Methylimidazolium Acetate Solutions Characterized by 31Mg β-Radiation-Detected NMR Spectroscopy

The complexation of MgII with adenosine 5′-triphosphate (ATP) is omnipresent in biochemical energy conversion, but is difficult to interrogate directly. Here we use the spin-urn:x-wiley:14337851:media:anie202207137:anie202207137-math-0001 β-emitter 31Mg to study MgII-ATP complexation in 1-ethyl-3-methylimidazolium acetate (EMIM-Ac) solutions using β-radiation-detected nuclear magnetic resonance (β-NMR). We demonstrate that (nuclear) spin-polarized 31Mg, following ion-implantation from an accelerator beamline into EMIM-Ac, binds to ATP within its radioactive lifetime before depolarizing. The evolution of the spectra with solute concentration indicates that the implanted 31Mg initially bind to the solvent acetate anions, whereafter they undergo dynamic exchange and form either a mono- (31Mg-ATP) or di-nuclear (31MgMg-ATP) complex. The chemical shift of 31Mg-ATP is observed up-field of 31MgMg-ATP, in accord with quantum chemical calculations. These observations constitute a crucial advance towards using β-NMR to probe chemistry and biochemistry in solution

Coming Together during COVID-19: A Mixed Methods Exploratory Study on Collective Efficacy in a State Developmental Disabilities Network

Background: Collective efficacy (CE) is a group’s shared belief that through their united efforts they can overcome challenges to achieve common goals (Bandura, 1993; 1997). CE has been shown to be related to professional growth, stress reduction, and overall collaborative impact in studies of groups responding to ongoing challenges as well as unforeseen circumstances (i.e. teachers, first-responders, and community responses to natural disasters) (Benight, 2004; Donohoo, 2016; Prati et al., 2011). COVID-19 has forced organizations serving individuals with disabilities to come together to adapt and change the ways in which they serve the disability community. Objective: This study examines reported attributes of CE as experienced by Arizona Developmental Disability Network (ADDN) members and their partners. This study respectively examines CE prior to COVID-19, currently, and predicted likelihood of CE attributes continuing in the future. Method: This mixed-method study involves data collected through a questionnaire and qualitative data collected through interviews with ADDN members and their partners. Results: The questionnaire results describe and compare the attributes of CE using sub-domains of social cohesion and trust, group competence, and enabling structures in three-time frames: prior to COVID-19, currently, and prediction of six months from now. The interviews provide narrative description of specific ADDN activities, roles, and perceptions; thus, adding a valuable dimension to interpreting the results. Conclusion: ADDN members and their partners have persisted and quickly adapted to COVID-19. These connections made during this time are likely to remain and help better serve Arizona’s disability community

Two pup vocalization types are genetically and functionally separable in deer mice

Vocalization is a widespread vertebrate social behavior that is essential for fitness in the wild. While many vocal behaviors are highly conserved, heritable features of specific vocalization types can vary both within and between species, raising the questions of why and how some vocal behaviors evolve. Here, using new computational tools to automatically detect and cluster vocalizations into distinct acoustic categories, we compare pup isolation calls across neonatal development in eight taxa of deer mice (genusPeromyscus) and compare them to laboratory mice (C57Bl6/j strain) and free-living, wild house mice (Mus musculus musculus). Whereas bothPeromyscusandMuspups produce ultrasonic vocalizations (USVs),Peromyscuspups also produce a second call type with acoustic features, temporal rhythms, and developmental trajectories that are distinct from those of USVs. In deer mice, these tonal and low frequency “cries” are predominantly emitted in postnatal days one through nine, while USVs are primarily made after day nine. Using playback assays, we show that cries result in a more rapid approach byPeromyscusmothers than USVs, suggesting a role for cries in eliciting parental care early in neonatal development. Using genetic crosses between two sister species of deer mice exhibiting large, innate differences in the acoustic structure of cries and USVs, we find that variation in vocalization rate, duration, and pitch display different degrees of genetic dominance and that cry and USV features can be uncoupled in second-generation hybrids. Taken together, this work shows that vocal behavior can evolve quickly between closely related rodent species in which vocalization types, likely serving distinct functions in communication, are controlled by distinct genetic loci

In the Supreme Court of the United States, October term 1965, no. ... original : State of South Carolina, plaintiff v. Nicholas deB. Katzenbach, Attorney General for the United States, defendant : motion for leave to file complaint.

This report is the motion for leave to file complaint by the defendant in South Carolina v. Katzenbach, 383 U.S. 301 (1966), a United States Supreme Court case in which the Court rejected a challenge from the state of South Carolina to the preclearance provisions of the Voting Rights Act of 1965, which required that some states submit changes in election districts to the Attorney General of the United States (at the time, Nicholas Katzenbach)