Effect of potassium and zinc on growth, yield and economics of sweet potato (Ipomoea batatas L.) cv. CO-34

A field experiment was conducted to study the effect of different levels of potassium and zinc on growth, yield and economics of sweet potato. The experiment was laid out on clay and loam soil by adopting randomized block design with factorial technique (FRBD). The sixteen treatments consisted of combination of four levels of po-tassium (0, 80, 100 and 120 kg/ha through muriate of potash and four levels of foliar zinc (control i.e. water spray, 10, 20 and 30ppm) through zinc sulphate. The individual application of potassium 120 kg K2O/ha significantly in-creased the number of tubers per plant (4.60), average weight of tuber (275.31 g), length of tuber (16.77 cm), diam-eter of tuber (5.69 cm), tuber yield per plot (9.71 kg), tuber yield per hectare (49.04 t) respectively as compared to control. With the foliar application of zinc (30 ppm) significant increase in number of tubers per plant (4.18), average weight of tuber (234.73 g), length of tuber (18.12 cm), diameter of tuber (5.16 cm), tuber yield per plot (8.33 kg) and tuber yield per hectare (42.05 t) was recordedas compared to control. The treatment combination (120 kg K2O+30ppm Zn) recorded the maximum yield parameters i.e. chlorophyll content (37.00 mg/100 g), average weight of tuber (302.17 g), length of tuber (19.82 cm), diameter of tuber (5.97 cm), maximum tuber yield per plot (11.02 kg), tuber yield per hectare (55.67 t) and benefit-cost ratio (B: C ratio) of 4.22:1.While, the treatment (120 kg K2O+30ppm Zn) had the maximum number of tuber (4.86), minimum number of tuber was recorded in control. From the experi-ment, it appeared that application of potassium and zinc can be used to improve yield and higher net monetary re-turns of sweet potato

Effect of pre-harvest spray of calcium nitrate, boric acid and zinc sulphate on yield and quality of Nagpur mandarin (Citrus reticulata Blanco)

An investigation was conducted on uniform, healthy, eight year old trees of Nagpur mandarin (citrus reticulata Blanco) at Fruit Research Farm, Department of Fruit Science at College of Horticulture and Forestry, Jhalawar during 16 September, 2014 to 1 March, 2015. Various doses of calcium nitrate (1.0%, 2.0% and 3.0 %), boric acid (0.2 %, 0.4 % and 0.6 %) and zinc sulphate (0.2 %, 0.4 % and 0.6 %) were sprayed before harvesting and compared with untreated ones. The results obtained indicated that the trees sprayed with T27 i.e. (calcium nitrate 3.0 % + boric acid 0.6 % + zinc sulphate 0.6 %) showed maximum increase diameter of fruit, fruit weight, fruit volume, number of fruits per plant, fruit yield per plant, estimated yield per hectare, reducing sugar, non reducing sugar, total sugar, juice per cent, sensory score and reduced peel thickness over control. Further, T24 treatment combination (calcium nitrate 3.0 % + boric acid 0.4 % + zinc sulphate 0.6 %) has also significantly increased number of segments per fruit, TSS, TSS: Acid ratio, ascorbic acid content, and reduced number of seeds per fruit and acidity percent of fruits

Effect of pre-harvest spray of calcium nitrate, boric acid and zinc sulphate on yield and quality of Nagpur mandarin (Citrus reticulata Blanco)

An investigation was conducted on uniform, healthy, eight year old trees of Nagpur mandarin (citrus reticulata Blanco) at FruitResearch Farm, Department of Fruit Science at College of Horticulture and Forestry, Jhalawar during 16 September, 2014 to 1 March, 2015.Various doses of calcium nitrate (1.0%, 2.0% and 3.0 %), boric acid (0.2 %, 0.4 % and 0.6 %) and zinc sulphate (0.2 %, 0.4 % and 0.6 %)were sprayed before harvesting and compared with untreated ones. The results obtained indicated that the trees sprayed with T27 i.e. (calciumnitrate 3.0 % + boric acid 0.6 % + zinc sulphate 0.6 %) showed maximum increase diameter of fruit, fruit weight, fruit volume, number offruits per plant, fruit yield per plant, estimated yield per hectare, reducing sugar, non reducing sugar, total sugar, juice per cent, sensory scoreand reduced peel thickness over control. Further, T24 treatment combination (calcium nitrate 3.0 % + boric acid 0.4 % + zinc sulphate 0.6 %)has also significantly increased number of segments per fruit, TSS, TSS: Acid ratio, ascorbic acid content, and reduced number of seeds perfruit and acidity percent of fruits

A Dual Receptor Crosstalk Model of G-Protein-Coupled Signal Transduction

Macrophage cells that are stimulated by two different ligands that bind to G-protein-coupled receptors (GPCRs) usually respond as if the stimulus effects are additive, but for a minority of ligand combinations the response is synergistic. The G-protein-coupled receptor system integrates signaling cues from the environment to actuate cell morphology, gene expression, ion homeostasis, and other physiological states. We analyze the effects of the two signaling molecules complement factors 5a (C5a) and uridine diphosphate (UDP) on the intracellular second messenger calcium to elucidate the principles that govern the processing of multiple signals by GPCRs. We have developed a formal hypothesis, in the form of a kinetic model, for the mechanism of action of this GPCR signal transduction system using data obtained from RAW264.7 macrophage cells. Bayesian statistical methods are employed to represent uncertainty in both data and model parameters and formally tie the model to experimental data. When the model is also used as a tool in the design of experiments, it predicts a synergistic region in the calcium peak height dose response that results when cells are simultaneously stimulated by C5a and UDP. An analysis of the model reveals a potential mechanism for crosstalk between the Gαi-coupled C5a receptor and the Gαq-coupled UDP receptor signaling systems that results in synergistic calcium release

Atomic force microscopy analysis of nanoparticles in non-ideal conditions

Nanoparticles are often measured using atomic force microscopy or other scanning probe microscopy methods. For isolated nanoparticles on flat substrates, this is a relatively easy task. However, in real situations, we often need to analyze nanoparticles on rough substrates or nanoparticles that are not isolated. In this article, we present a simple model for realistic simulations of nanoparticle deposition and we employ this model for modeling nanoparticles on rough substrates. Different modeling conditions (coverage, relaxation after deposition) and convolution with different tip shapes are used to obtain a wide spectrum of virtual AFM nanoparticle images similar to those known from practice. Statistical parameters of nanoparticles are then analyzed using different data processing algorithms in order to show their systematic errors and to estimate uncertainties for atomic force microscopy analysis of nanoparticles under non-ideal conditions. It is shown that the elimination of user influence on the data processing algorithm is a key step for obtaining accurate results while analyzing nanoparticles measured in non-ideal conditions

Counter-current chromatography for the separation of terpenoids: A comprehensive review with respect to the solvent systems employed

Copyright @ 2014 The Authors.This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.Natural products extracts are commonly highly complex mixtures of active compounds and consequently their purification becomes a particularly challenging task. The development of a purification protocol to extract a single active component from the many hundreds that are often present in the mixture is something that can take months or even years to achieve, thus it is important for the natural product chemist to have, at their disposal, a broad range of diverse purification techniques. Counter-current chromatography (CCC) is one such separation technique utilising two immiscible phases, one as the stationary phase (retained in a spinning coil by centrifugal forces) and the second as the mobile phase. The method benefits from a number of advantages when compared with the more traditional liquid-solid separation methods, such as no irreversible adsorption, total recovery of the injected sample, minimal tailing of peaks, low risk of sample denaturation, the ability to accept particulates, and a low solvent consumption. The selection of an appropriate two-phase solvent system is critical to the running of CCC since this is both the mobile and the stationary phase of the system. However, this is also by far the most time consuming aspect of the technique and the one that most inhibits its general take-up. In recent years, numerous natural product purifications have been published using CCC from almost every country across the globe. Many of these papers are devoted to terpenoids-one of the most diverse groups. Naturally occurring terpenoids provide opportunities to discover new drugs but many of them are available at very low levels in nature and a huge number of them still remain unexplored. The collective knowledge on performing successful CCC separations of terpenoids has been gathered and reviewed by the authors, in order to create a comprehensive document that will be of great assistance in performing future purifications. © 2014 The Author(s)

Dark Matter Search Results from the PICO-60C(3)F(8) Bubble Chamber

[EN] New results are reported from the operation of the PICO-60 dark matter detector, a bubble chamber filled with 52 kg of C3F8 located in the SNOLAB underground laboratory. As in previous PICO bubble chambers, PICO-60 C3F8 exhibits excellent electron recoil and alpha decay rejection, and the observed multiple-scattering neutron rate indicates a single-scatter neutron background of less than one event per month. A blind analysis of an efficiency-corrected 1167-kg day exposure at a 3.3-keV thermodynamic threshold reveals no single-scattering nuclear recoil candidates, consistent with the predicted background. These results set the most stringent direct-detection constraint to date on the weakly interacting massive particle (WIMP)-proton spin-dependent cross section at 3.4 x 10(-41) cm(2) for a 30-GeVc(-2) WIMP, more than 1 order of magnitude improvement from previous PICO results.The PICO Collaboration wishes to thank SNOLAB and its staff for support through underground space, logistical, and technical services. SNOLAB operations are supported by the Canada Foundation for Innovation and the Province of Ontario Ministry of Research and Innovation, with underground access provided by Vale at the Creighton mine site. We are grateful to Kristian Hahn and Stanislava Sevova of Northwestern University and Bjorn Penning of the University of Bristol for their assistance and useful discussion. We wish to acknowledge the support of the Natural Sciences and Engineering Research Council of Canada (NSERC) and the Canada Foundation for Innovation (CFI) for funding. We acknowledge the support from National Science Foundation (NSF) (Grants No. 0919526, No. 1506337, No. 1242637, and No. 1205987). We acknowledge that this work is supported by the U.S. Department of Energy (DOE) Office of Science, Office of High Energy Physics (under Award No. DE-SC-0012161), by a DOE Office of Science Graduate Student Research (SCGSR) award, by Direccion General Asuntos del Personal Academico, Universidad Nacional Autonoma de Mexico (DGAPA-UNAM) through the grant Programa de Apoyo a Proyectos de Investigacion e Innovacion Tecnologica (PAPIIT) No. IA100316 and by Consejo Nacional de Ciencia y Tecnologia (CONACyT) (Mexico) through Grant No. 252167, by the Department of Atomic Energy (DAE), the Government of India, under the Center of AstroParticle Physics II project (CAPP-II) at Saha Institute of Nuclear Physics (SINP), by the Czech Ministry of Education, Youth and Sports (Grant No. LM2015072), and by the Spanish Ministerio de Economia y Competitividad, Consolider MultiDark (Grant No. CSD2009-00064). This work is partially supported by the Kavli Institute for Cosmological Physics at the University of Chicago through NSF Grant No. 1125897, and an endowment from the Kavli Foundation and its founder Fred Kavli. We also wish to acknowledge the support from Fermi National Accelerator Laboratory under Contract No. De-AC02-07CH11359, and Pacific Northwest National Laboratory, which is operated by Battelle for the U.S. Department of Energy under Contract No. DE-AC05-76RL01830. We also thank Compute Canada and the Center for Advanced Computing, ACENET, Calcul Quebec, Compute Ontario, and WestGrid for the computational support.Amole, C.; Ardid Ramírez, M.; Arnquist, I.; Asner, DM.; Baxter, D.; Behnke, E.; Bhattacharjee, P.... (2017). Dark Matter Search Results from the PICO-60C(3)F(8) Bubble Chamber. Physical Review Letters. 118(25). https://doi.org/10.1103/PhysRevLett.118.251301S11825Olive, K. A. (2014). Review of Particle Physics. 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Spin-Dependent Weakly-Interacting-Massive-Particle–Nucleon Cross Section Limits from First Data of PandaX-II Experiment. Physical Review Letters, 118(7). doi:10.1103/physrevlett.118.071301Aartsen, M. G., Ackermann, M., Adams, J., Aguilar, J. A., Ahlers, M., Ahrens, M., … Ansseau, I. (2017). Search for annihilating dark matter in the Sun with 3 years of IceCube data. The European Physical Journal C, 77(3). doi:10.1140/epjc/s10052-017-4689-9Tanaka, T., Abe, K., Hayato, Y., Iida, T., Kameda, J., Koshio, Y., … Nakahata, M. (2011). AN INDIRECT SEARCH FOR WEAKLY INTERACTING MASSIVE PARTICLES IN THE SUN USING 3109.6 DAYS OF UPWARD-GOING MUONS IN SUPER-KAMIOKANDE. The Astrophysical Journal, 742(2), 78. doi:10.1088/0004-637x/742/2/78Choi, K., Abe, K., Haga, Y., Hayato, Y., Iyogi, K., Kameda, J., … Nakahata, M. (2015). Search for Neutrinos from Annihilation of Captured Low-Mass Dark Matter Particles in the Sun by Super-Kamiokande. Physical Review Letters, 114(14). doi:10.1103/physrevlett.114.141301Roszkowski, L., Austri, R. R. de, & Trotta, R. (2007). Implications for the Constrained MSSM from a new prediction forb→sγ. Journal of High Energy Physics, 2007(07), 075-075. doi:10.1088/1126-6708/2007/07/075Akerib, D. S., Araújo, H. M., Bai, X., Bailey, A. J., Balajthy, J., Beltrame, P., … Boulton, E. M. (2016). Results on the Spin-Dependent Scattering of Weakly Interacting Massive Particles on Nucleons from the Run 3 Data of the LUX Experiment. Physical Review Letters, 116(16). doi:10.1103/physrevlett.116.161302Aprile, E., Aalbers, J., Agostini, F., Alfonsi, M., Amaro, F. D., Anthony, M., … Bauermeister, B. (2016). XENON100 dark matter results from a combination of 477 live days. Physical Review D, 94(12). doi:10.1103/physrevd.94.122001Adrián-Martínez, S., Albert, A., André, M., Anton, G., Ardid, M., Aubert, J.-J., … Basa, S. (2016). Limits on dark matter annihilation in the sun using the ANTARES neutrino telescope. Physics Letters B, 759, 69-74. doi:10.1016/j.physletb.2016.05.019Adrián-Martínez, S., Albert, A., André, M., Anton, G., Ardid, M., Aubert, J.-J., … Basa, S. (2016). A search for Secluded Dark Matter in the Sun with the ANTARES neutrino telescope. Journal of Cosmology and Astroparticle Physics, 2016(05), 016-016. doi:10.1088/1475-7516/2016/05/016Akerib, D. S., Alsum, S., Araújo, H. M., Bai, X., Bailey, A. J., Balajthy, J., … Biesiadzinski, T. P. (2017). Results from a Search for Dark Matter in the Complete LUX Exposure. Physical Review Letters, 118(2). doi:10.1103/physrevlett.118.021303Tan, A., Xiao, M., Cui, X., Chen, X., Chen, Y., Fang, D., … Gong, H. (2016). Dark Matter Results from First 98.7 Days of Data from the PandaX-II Experiment. Physical Review Letters, 117(12). doi:10.1103/physrevlett.117.121303Angloher, G., Bento, A., Bucci, C., Canonica, L., Defay, X., Erb, A., … Zöller, A. (2016). Results on light dark matter particles with a low-threshold CRESST-II detector. The European Physical Journal C, 76(1). doi:10.1140/epjc/s10052-016-3877-3Agnese, R., Anderson, A. J., Aramaki, T., Asai, M., Baker, W., Balakishiyeva, D., … Billard, J. (2016). New Results from the Search for Low-Mass Weakly Interacting Massive Particles with the CDMS Low Ionization Threshold Experiment. Physical Review Letters, 116(7). doi:10.1103/physrevlett.116.071301Agnes, P., Agostino, L., Albuquerque, I. F. M., Alexander, T., Alton, A. K., Arisaka, K., … Bonfini, G. (2016). Results from the first use of low radioactivity argon in a dark matter search. Physical Review D, 93(8). doi:10.1103/physrevd.93.081101Agnese, R., Anderson, A. J., Asai, M., Balakishiyeva, D., Basu Thakur, R., Bauer, D. A., … Bowles, M. A. (2014). Search for Low-Mass Weakly Interacting Massive Particles with SuperCDMS. Physical Review Letters, 112(24). doi:10.1103/physrevlett.112.241302Agnese, R., Anderson, A. J., Asai, M., Balakishiyeva, D., Barker, D., Basu Thakur, R., … Bowles, M. A. (2015). 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A Global Clustering Algorithm to Identify Long Intergenic Non-Coding RNA - with Applications in Mouse Macrophages

Identification of diffuse signals from the chromatin immunoprecipitation and high-throughput massively parallel sequencing (ChIP-Seq) technology poses significant computational challenges, and there are few methods currently available. We present a novel global clustering approach to enrich diffuse CHIP-Seq signals of RNA polymerase II and histone 3 lysine 4 trimethylation (H3K4Me3) and apply it to identify putative long intergenic non-coding RNAs (lincRNAs) in macrophage cells. Our global clustering method compares favorably to the local clustering method SICER that was also designed to identify diffuse CHIP-Seq signals. The validity of the algorithm is confirmed at several levels. First, 8 out of a total of 11 selected putative lincRNA regions in primary macrophages respond to lipopolysaccharides (LPS) treatment as predicted by our computational method. Second, the genes nearest to lincRNAs are enriched with biological functions related to metabolic processes under resting conditions but with developmental and immune-related functions under LPS treatment. Third, the putative lincRNAs have conserved promoters, modestly conserved exons, and expected secondary structures by prediction. Last, they are enriched with motifs of transcription factors such as PU.1 and AP.1, previously shown to be important lineage determining factors in macrophages, and 83% of them overlap with distal enhancers markers. In summary, GCLS based on RNA polymerase II and H3K4Me3 CHIP-Seq method can effectively detect putative lincRNAs that exhibit expected characteristics, as exemplified by macrophages in the study

Search for charged-lepton flavor violation in Υ(2S)→ℓ∓τ±\Upsilon(2S) \to \ell^\mp\tau^\pm (ℓ=e,μ\ell=e,\mu) decays at Belle

We report a search for the charged-lepton flavor violation in $\Upsilon(2S) \to \ell^\mp\tau^\pm$ ($\ell=e,\mu$) decays using a $25~\mathrm{fb}^{-1}$ $\Upsilon(2S)$ sample collected by the Belle detector at the KEKB $e^{+}e^{-}$ asymmetric-energy collider. We find no evidence for a signal and set upper limits on the branching fractions ($\mathcal{B}$) at 90$\%$ confidence level. We obtain the most stringent upper limits: $\mathcal{B}(\Upsilon(2S) \to \mu^{\mp}\tau^{\pm}) < 0.26 \times 10^{-6}$ and $\mathcal{B}(\Upsilon(2S) \to e^{\mp}\tau^{\pm}) < 1.02 \times 10^{-6}$.Comment: 11 pages, 3 figures, Submitted to JHE