3 research outputs found
Mass Spectrometry Imaging on Porous Silicon: Investigating the Distribution of Bioactives in Marine Mollusc Tissues
Desorption/ionization on porous silicon-mass spectrometry
(DIOS-MS)
is an attractive alternative to conventional matrix-assisted laser
desorption/ionization mass spectrometry (MALDI-MS) for the analysis
of low molecular weight compounds. Porous silicon (pSi) chips are
also suitable as support for mass spectrometry imaging (MSI). Here,
we report an implementation of DIOS-MSI using the biosynthetic organs
of a marine mollusc for proof of principle. The tissue section is
stamped onto a fluorocarbon-functionalized pSi chip, which extracts
and traps small hydrophobic molecules from the tissue under retention
of their relative spatial distribution. The section is subsequently
removed and the chip is imaged without any remaining tissue. We apply
this novel tissue contact printing approach to investigate the distribution
of biologically active brominated precursors to Tyrian purple in the
hypobranchial gland of the marine mollusc, <i>Dicathais orbita,</i> using DIOS–MSI. The tissue contact printing is also compatible
with other types of desorption/ionization surfaces, such as nanoassisted
laser desorption/ionization (NALDI) targets
L'enquête multi-disciplinaire entreprise dans le Finistère
Maho Jacques. L'enquête multi-disciplinaire entreprise dans le Finistère. In: Revue française de sociologie, 1963, 4-3. pp. 321-325
The Anti-Obesity Compound Asperuloside Reduces Inflammation in the Liver and Hypothalamus of High-Fat-Fed Mice
Obesity produces a systemic low-grade inflammation associated with many adverse health conditions and, as we recently learned, with complications of COVID-19. Functional studies in animal models have demonstrated that asperuloside, an iridoid glycoside found in many medicinal plants, has produced promising anti-obesity results. However, the safety profile and the anti-inflammatory properties of asperuloside remain unknown. Here, we confirmed the previously reported anti-obesity properties of asperuloside, and, importantly, we performed toxicity studies assessing cell viability providing a dose reference for future animal experiments. Asperuloside significantly reduced blood levels of leptin and the mRNA levels of orexigenic peptides, such as NPY and AgRP in mice consuming HFD, with no effect on mice eating a standard chow diet. In addition, our results indicate that ASP reduced both hypothalamic and hepatic mRNA levels of pro-inflammatory cytokines such as IL-1, IL-6 and TNF-α as well as the blood levels of plasminogen activator inhibitor-1 (PAI-1), which are known to play a major role in the development of insulin resistance and cardiovascular complications. Collectively, our findings suggest that asperuloside is a safe compound for long-term use in animal models and that it reduces the elevated levels of pro-inflammatory cytokines occurring in obesity