12 research outputs found
Közösségfejlesztés a Magyarországi Baptista Egyházban
Szakdolgozatom tĂ©májakĂ©nt a Baptista Egyházban folyĂł közössĂ©gfejlesztĂ©s elemzĂ©sĂ©t választottam. A közössĂ©gfejlesztĂ©s napjainkban a szociolĂłgiának egyre nagyobb teret hĂłdĂtĂł kutatási terĂĽlete. Manapság kĂĽlönbözĹ‘ közössĂ©gek fejlesztĂ©sĂ©re van lehetĹ‘sĂ©g. Hallhatunk iskolák, szervezetek, vállalatok, egyházak vagy Ă©ppen telepĂĽlĂ©sek kĂĽlönfĂ©le közössĂ©geinek fejlesztĂ©sĂ©rĹ‘l. Ezen tevĂ©kenysĂ©gek fontosak a társadalmi csoportok kohĂ©ziĂłjának az elĹ‘segĂtĂ©sĂ©hez Ă©s fenntartásához.
Dolgozatom cĂ©lja, hogy kĂ©pet adjak a Baptista Egyház közössĂ©gĂ©inek működĂ©sĂ©rĹ‘l, összetĂ©telĂ©rĹ‘l, kialakulásárĂłl Ă©s Ă©rtĂ©krendszerĂ©rĹ‘l. A kutatásom cĂ©lja az interjĂş alanyok válaszain keresztĂĽl bemutatni a baptista gyĂĽlekezeteken belĂĽli közössĂ©gek kapcsolatainak rendszerĂ©t, összetettsĂ©gĂ©t, valamint azt, hogy a válaszadĂłk mikĂ©nt vállalnak szerepet a gyĂĽlekezetĂĽk közössĂ©gĂ©nek Ă©pĂtĂ©sĂ©ben.
Kutatási módszerként az interjút választottam és használtam. Félig strukturált interjúkkal dolgoztam.
Szakdolgozatomban három hipotézist fogalmaztam meg, amelyekre a válaszokat a feltett kutatási kérdéseim alapján kaptam meg. Hipotéziseim részben igaznak bizonyultak. A gyülekezetbe járó tagok szeretnék, ha közösségük folyamatosan fejlődne, kapcsolataik erősödne.BSc/BASzociológi
Stu1p Is Physically Associated with β-Tubulin and Is Required for Structural Integrity of the Mitotic Spindle
Formation of the bipolar mitotic spindle relies on a balance of forces acting on the spindle poles. The primary outward force is generated by the kinesin-related proteins of the BimC family that cross-link antiparallel interpolar microtubules and slide them past each other. Here, we provide evidence that Stu1p is also required for the production of this outward force in the yeast Saccharomyces cerevisiae. In the temperature-sensitive stu1–5 mutant, spindle pole separation is inhibited, and preanaphase spindles collapse, with their previously separated poles being drawn together. The temperature sensitivity of stu1–5 can be suppressed by doubling the dosage of Cin8p, a yeast BimC kinesin–related protein. Stu1p was observed to be a component of the mitotic spindle localizing to the midregion of anaphase spindles. It also binds to microtubules in vitro, and we have examined the nature of this interaction. We show that Stu1p interacts specifically with β-tubulin and identify the domains required for this interaction on both Stu1p and β-tubulin. Taken together, these findings suggest that Stu1p binds to interpolar microtubules of the mitotic spindle and plays an essential role in their ability to provide an outward force on the spindle poles
Structure of the Golgi and Distribution of Reporter Molecules at 20°C Reveals the Complexity of the Exit Compartments
Incubating cells at 20°C blocks transport out of the Golgi complex and amplifies the exit compartments. We have used the 20°C block, followed by EM tomography and serial section reconstruction, to study the structure of Golgi exit sites in NRK cells. The dominant feature of Golgi structure in temperature-blocked cells is the presence of large bulging domains on the three trans-most cisternae. These domains extend laterally from the stack and are continuous with “cisternal” domains that maintain normal thickness and alignment with the other stacked Golgi cisternae. The bulging domains do not resemble the perpendicularly extending tubules associated with the trans-cisternae of control cells. Such tubules are completely absent in temperature-blocked cells. The three cisternae with bulging domains can be identified as trans by their association with specialized ER and the presence of clathrin-coated buds on the trans-most cisterna only. Immunogold labeling and immunoblots show a significant degradation of a medial- and a trans-Golgi marker with no evidence for their redistribution within the Golgi or to other organelles. These data suggest that exit from the Golgi occurs directly from three trans-cisternae and that specialized ER plays a significant role in trans-Golgi function