Identifikasi Genetik Menggunakan Marker Mikrosatelit Dan Hubungannya Dengan Sifat Kuantitatif Pada Sapi

The occurrence of genetic changes in Bali and Brangus cattle crossing and it\u27s relationship to quantitative traits (e.g. growth and birth weight) were identified using microsatellite markers. Sixteen microsatellite loci were amplified using polymerase chain reaction (PCR) technique, the amplified products were then separated on vertical discontinuous polyacrylamide gel electrophoresis (PAGE) and silver-staining method was applied to detect the fragment. The evaluation to the stability and allele inheritance pattern were checked using Chi-Square segregation analysis. The results of this research showed that allele numbers average detected in Brangus and Bali cattle and their progeny were 2.31, 2.56, and 2.75 respectively. At most microsatellite loci, the allele distributions incline to concentrate and to form bimodal trend. Of 16 micro satellite loci tested, 5 microsatelitte markers namely INRA 037, HEL 9, CSSM 66, INRA 035 and ETH 225 indicated a significant response to average daily gain. In terms of birth weight, a significant response was shown by INRA 037

Synthesis of Gd-dtpa-folat for Magnetic Resonance Imaging Contrast Agent and Characterization by Using 153gd-dtpa-folate Radioactive

Contrast agent was used to clarify the image of the organ that is difficult to distinguish by MRI (Magnetic Resonance Imaging) techniques, particularly in soft tissues of the central nervous system, liver, digestive system, lymphatic system, breast, cardiovascular and pulmonary systems. One of the commonly used contrast agents in hospitals is Gadolinium-DieThylenetriamine Pentaacetic Acid (Gd-DTPA). Gd-DTPA is non specific contrast agent, therefore it has led to develop a contrast agent that is able to achieve the target without defect to the surrounding normal cells. Folic acid as a safe vitamin, inexpensive, specific and also can be used as drug delivery. This study was conducted in the development of targeted MRI contrast agent based of folic acid as a carrier by using Gd metal and DTPA ligand. Since Gd-DTPA-folate is non-active compounds, the characterization of Gd-DTPA-folate was performed using 153Gd-DTPA-folate, which was obtained by reacting DTPA-folate with radionuclides 153Gd. The results of optimization reactions of 153Gd-DTPA-folate was obtained at the mole ratio of DTPA-folate to Gd metal 20:1 and produced the radiochemical purity more than 90%. Identification of the non active product Gd-DTPA-folate was performed using an Fourier Transform- Infra Red (FT-IR) Spectrophotometer and Thin Layer Chromatography (TLC) compared with folic acid and EDA-folate. The results indicated that the product is Gd-DTPA-folate

A Novel Single Nucleotide Polymorphism in Exon 4 of Insulin-Like Growth Factor-1 Associated with Production Traits in Bali Cattle

Insulin-like growth factor-1 (IGF-1) is one of the gene candidates that can be used in selection strategy by using DNA markers (marker assisted selection). Gene candidate strategy is a molecular biology techniques to identify quantitative trait loci directly, with the assumption that genetic variation associated to quantitative trait variation. This study was designed to identify any new mutations in exon 4 that can cause the IGF-1 gene polymorphism and then affect the production traits on Bali cattle. Single nucleotide polymorphism (SNP) discovery was conducted by using the direct sequencing technique. Genetic variation of the genes candidate was identified by using PCR-RFLP technique. The results of this study indicate the presence of a new SNP in exon 4 of IGF-1 gene caused by the T/C transition, which can be identified using Rsa1 restriction enzyme. Genotypic polymorphism of IGF-1/Rsa1 has a significant influence on birth weight, weaning weight and average daily gain of Bali cattle. CC genotype had a birth weight rate, weaning weight and average daily gain of: 15.64±1.83; 83.15±9.00, and 0.439±0.07 respectively, higher than the TT and CT genotype. IGF-1/Rsa1 can be used as a genetic marker for selection of birth weight, weaning weight, and daily body weight gain

Reading Fatwas of MUI a Perspective of Maslahah Concept

Abstrak: Kemaslahatan dan kebaikan manusia merupakan tujuan pokok setiap bentuk peratuan, termasuk fatwa-fatwa Majelis Ulama Indonesia (MUI). Salah satubidang fatwa yang diterbitkan MUI adalahhukumkeluarga. Fokusutamatulisaniniadalahmembacaulang dan menganalisa fatwa-fatwa MUI dalambidanghukumkeluargadenganperspektifkonsepmaslahatmayoritas fuqaha yang juga telahditerbitkandalam Fatwa MUI tentangKriteriaMaslahatNomor: 6/MUNAS VII/MUI/10/2005. Fatwa-fatwa tersebut, dilihatmenggunakanparadigmakonsepmaslahat, adakalanya masuk dalam kategori maṣlaḥahmu‘tabarah, maṣlaḥahmursalah, dan adaindikasibeberapa fatwa yang masuk kedalam kategori maṣlaḥah mulghah. Adapun fatwa MUI dalam bidang hukum keluarga yang dimaksud dalam tulisan ini yaitu fatwa tentang Aborsi, tentang Perkawinan Beda Agama, tentang Kewarisan Beda Agama, tentang Perkawinan Di BawahTangan (sirri, tidak dicatatkan), dan tentang Nikah Wisata.Kata kunci: Maṣlaḥah, GradasiMaṣlaḥah, MUI, HukumKeluargaAbstract: Human benefit and goodness are the main objectives of every form of regulation, including the fatwas of the Council of Indonesian Ulama (MUI). One of the fatwa fields issued by MUI is family law. The main focus of this paper is to reread and analyze the fatwas of the MUI in the field of family law with the perspective of the concept of majority fuqaha masses which have also been published in the MUI Fatwa concerning the Maslahat No. 6 / MUNAS VII / MUI / 10/2005. These fatwas, are seen using the concept of maslahat, sometimes include to the category of maṣlaḥahmu‘tabarah, maṣlaḥahmursalah, and there are indications of several fatwas that include to the category of maṣlaḥahmulghah. The fatwas of the MUI in the field of family law referred to in this article is a fatwa on Abortion, concerning Differential Marriage, about Different Religions, About Marriage Under the Hand (sirri, not recorded), and about Tourism Marriage.Keywords: Maṣlaḥah, Gradation of Maṣlaḥah, MUI, Family La

Organoleptic Value and Cholesterol Content of Shredded Meat of Rejected Layers Based on Immersion Level of Lime Juice (Citrus aurantifolia S.)

The production of shredded meat is an innovation to improve the structure of rejected layers of meat into processed products that are tender and low in cholesterol. This study aims to determine the effect of the immersion level of lime juice (Citrus aurantifolia S.). On the organoleptic value and cholesterol of shredded hens of rejected layers. The study was carried out using a randomized block design in the same direction with four treatments and tree replications, namely P0 = meat without soaking in Citrus aurantifolia S liquid; P1= meat soaked in 200 ml of Citrus aurantifolia S liquid, P2= meat soaked in 250 ml of Citrus aurantifolia S liquid and P3= meat soaked in 300 ml of Citrus aurantifolia S liquid. The research data were analyzed using the Analysis of Variance and continued with Duncan's Multiple Range Test using the SPSS 25 program. The results showed that soaking meat in Citrus aurantifolia S liquid in the production of shredded culled laying hens had no significant effect (p>0.05) on organoleptic quality (aroma, tenderness, taste, and acceptance), significantly (p<0.05) can improve the shredded structure according to the Indonesian National Standard (SNI: 1995). Conclusion: Soaking culled laying hens in Citrus aurantifolia S. liquid at a level of 200-300 ml of meat weight can produce shredded meat with a pleasant aroma, tender, tasty, soft, and acceptable to panelists with normal cholesterol levels, namely 10.00 - 46.66 mg/dl