17 research outputs found

    Increased Biodiversity in the Environment Improves the Humoral Response of Rats

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    Previous studies have compared the immune systems of wild and of laboratory rodents in an effort to determine how laboratory rodents differ from their naturally occurring relatives. This comparison serves as an indicator of what sorts of changes might exist between modern humans living in Western culture compared to our hunter-gatherer ancestors. However, immunological experiments on wild-caught animals are difficult and potentially confounded by increased levels of stress in the captive animals. In this study, the humoral immune responses of laboratory rats in a traditional laboratory environment and in an environment with enriched biodiversity were examined following immunization with a panel of antigens. Biodiversity enrichment included colonization of the laboratory animals with helminths and co-housing the laboratory animals with wild-caught rats. Increased biodiversity did not apparently affect the IgE response to peanut antigens following immunization with those antigens. However, animals housed in the enriched biodiversity setting demonstrated an increased mean humoral response to T-independent and T-dependent antigens and increased levels of “natural” antibodies directed at a xenogeneic protein and at an autologous tissue extract that were not used as immunogens

    Ovarian Cancer: Early Symptom Patterns

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    Measuring Change During Behavioral Parent Training Using the Parent Instruction-Giving Game with Youngsters (PIGGY): A Clinical Replication

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    This is the second study to investigate the clinical use of the Parent-Instruction Game with Youngsters (PIGGY) which is a structured observation system derived from the Dyadic Parent-Child Interaction Coding System II (DPICS-II; Eyberg, Bessmer, Newcomb, Edwards, & Robinson, 1994) and the Behavior Coding System (BCS; Forehand & McMahon, 1981). In a previous study, the PIGGY demonstrated strong reliability and validity as well as clinical utility (Hupp, Reitman, Forde, Shriver, & Kelley, 2008). The present study is a replication of the previous research on clinical utility by using the PIGGY to monitor changes in parent and child behavior during and after behavioral parent trainin

    Adaptation in a Mouse Colony Monoassociated with Escherichia coli K-12 for More than 1,000 Daysâ–ż

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    Although mice associated with a single bacterial species have been used to provide a simple model for analysis of host-bacteria relationships, bacteria have been shown to display adaptability when grown in a variety of novel environments. In this study, changes associated with the host-bacterium relationship in mice monoassociated with Escherichia coli K-12 over a period of 1,031 days were evaluated. After 80 days, phenotypic diversification of E. coli was observed, with the colonizing bacteria having a broader distribution of growth rates in the laboratory than the parent E. coli. After 1,031 days, which included three generations of mice and an estimated 20,000 generations of E. coli, the initially homogeneous bacteria colonizing the mice had evolved to have widely different growth rates on agar, a potential decrease in tendency for spontaneous lysis in vivo, and an increased tendency for spontaneous lysis in vitro. Importantly, mice at the end of the experiment were colonized at an average density of bacteria that was more than 3-fold greater than mice colonized on day 80. Evaluation of selected isolates on day 1,031 revealed unique restriction endonuclease patterns and differences between isolates in expression of more than 10% of the proteins identified by two-dimensional electrophoresis, suggesting complex changes underlying the evolution of diversity during the experiment. These results suggest that monoassociated mice might be used as a tool for characterizing niches occupied by the intestinal flora and potentially as a method of targeting the evolution of bacteria for applications in biotechnology

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    <p>(A) Rat muscle extracts were separated by SDS PAGE and probed by immunoblotting as described in the Methods. The analysis was limited to 15 animals (n = 8 biome enriched; lanes E1 through E8, and n = 7 biome depleted; lanes D1 through D7) due to size constraints of the gel. A control strip with no serum is labeled “C”, and indicates reactivity of the anti-IgM conjugate with muscle-derived antigens. (B) The number of bands recognized by natural IgM in individual sera (p = 0.0089) and the total reactivity of natural IgM from each serum sample (p = 0.0093) are shown, with the bars indicating the mean and standard error. (C) The distribution of bands as a function of band size is shown. For this analysis, the average number of bands in biome depleted and biome enriched rats (Y-axis) was plotted on linear and log scales (main figure and figure inset, respectively) versus different band sizes (X-axis).</p

    Relative concentration of DNP-specific antibody in the serum of biome depleted (n = 20) and biome enriched (n = 15) rats.

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    <p>The relative concentration of antibody was determined by ELISA as described in the Methods. Relative levels of (A) IgM, (B) IgG, and (C) subclasses of IgG are shown. The means and standard errors are shown. The <i>p</i>-values associated with comparing data from biome depleted and biome enriched animals using a t-test are shown. (NS = not significant)</p

    Natural anti-human serum albumin antibody levels in the serum of biome depleted (n = 20) and biome enriched (n = 15) rats.

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    <p>The relative concentration of antibody was determined by ELISA as described in the Methods. Relative levels of (A) IgM and (B) IgG are shown. Binding to human serum albumin (HSA) was used as a measure of reactivity toward a xenogeneic antigen for which the animals lacked previous exposure. The means, standard errors, and the <i>p</i>-values associated with comparing data from biome depleted and biome enriched animals using a t-test are shown. (NS = not significant)</p
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