Jogo do bicho, estado e cidadania: rupturas e continuidades no tempo de Vargas

A lógica de funcionamento de cada uma das instituições que compõem o aparelho jurídico do Estado – que compreende todas as corporações policiais/militares, os tribunais e os sistemas carcerários/punitivos – permite recuperar a forma como é realizada a cidadania em dado período histórico. Partindo da comparação da ação da Polícia e dos tribunais do Trabalho e da Justiça com o fenômeno social conhecido como jogo do bicho foi possível perceber que, por trás das contradições aparentes, se preservou formas de controle social e relações de dominação existentes antes do advento da carteira de trabalho

An outlook of drug consumption in Brazil in ninetienth century

Objetivo. O objetivo do presente artigo é compreender a natureza do consumo de psicoativos na sociedade brasileira escravista do século XIX. Metodologia. A metodologia utilizada é a histórica, que consiste em problematizar conceitos de outras áreas a partir do material empírico do passado. Resultados. Os resultados da pesquisa mostraram que o Brasil participou do fenômeno mundial chamado de revolução psicoativa, tanto como consumidor, quanto como fornecedor de drogas para o mercado mundial. Conclusões. Conclui-se dessa exposição que o uso de drogas de forma expandida é parte integrante da modernidade.Objective. The purpose of this article is to understand the nature of the consumption of psychoactive drugs in Brazilian slave society of the nineteenth century. Methodology. The methodology used is historical, which consists in problematizing concepts from other areas from the empirical material of the past. Results. The research results showed that Brazil participated in the worldwide phenomenon called psychoactive revolution, both as a consumer and as a drug supplier to the world market. Conclusions. It is concluded that the use of drugs in an expanded way is an integral part of modern times

Notch Mediates Inter-tissue Communication to Promote Tumorigenesis.

Disease progression in many tumor types involves the interaction of genetically abnormal cancer cells with normal stromal cells. Neoplastic transformation in a Drosophila genetic model of epidermal growth factor receptor (EGFR)-driven tumorigenesis similarly relies on the interaction between epithelial and mesenchymal cells, providing a simple system to investigate mechanisms used for the cross-talk. Using the Drosophila model, we show that the transformed epithelium hijacks the mesenchymal cells through Notch signaling, which prevents their differentiation and promotes proliferation. A key downstream target in the mesenchyme is Zfh1/ZEB. When Notch or zfh1 are depleted in the mesenchymal cells, tumor growth is compromised. The ligand Delta is highly upregulated in the epithelial cells where it is found on long cellular processes. By using a live transcription assay in cultured cells and by depleting actin-rich processes in the tumor epithelium, we provide evidence that signaling can be mediated by cytonemes from Delta-expressing cells. We, thus, propose that high Notch activity in the unmodified mesenchymal cells is driven by ligands produced by the cancerous epithelial. This long-range Notch signaling integrates the two tissues to promote tumorigenesis, by co-opting a normal regulatory mechanism that prevents the mesenchymal cells from differentiating

The nucleolar protein Viriato/Nol12 is required for the growth and differentiation progression activities of the Dpp pathway during Drosophila eye development

Drosophila Decapentaplegic (Dpp), a member of the BMP2/4 class of the TGF-βs, is required for organ growth, patterning and differentiation. However, much remains to be understood about the mechanisms acting downstream of these multiple roles. Here we investigate this issue during the development of the Drosophila eye. We have previously identified viriato (vito) as a dMyc-target gene encoding a nucleolar protein that is required for proper tissue growth in the developing eye. By carrying out a targeted in vivo double-RNAi screen to identify genes and pathways functioning with Vito during eye development, we found a strong genetic interaction between vito and members of the Dpp signaling pathway including the TGF-β receptors tkv (type I), put (type II), and the co-Smad medea (med). Analyzing the expression of the Dpp receptor Tkv and the activation pattern of the pathway’s transducer, p-Mad, we found that vito is required for a correct signal transduction in Dpp-receiving cells. Overall, we validate the use of double RNAi to find specific genetic interactions and, in particular, we uncover a link between the Dpp pathway and Vito, a nucleolar component. vito would act genetically downstream of Dpp, playing an important role in maintaining a sufficient level of Dpp activity for the promotion of eye disc growth and regulation of photoreceptor differentiation in eye development

Targeting of Fzr/Cdh1 for timely activation of the APC/C at the centrosome during mitotic exit.

A multi-subunit ubiquitin ligase, the anaphase-promoting complex/cyclosome (APC/C), regulates critical cellular processes including the cell cycle. To accomplish its diverse functions, APC/C activity must be precisely regulated in time and space. The interphase APC/C activator Fizzy-related (Fzr or Cdh1) is localized at centrosomes in animal cells. However, neither the mechanism of its localization nor its importance is clear. Here we identify the centrosome component Spd2 as a major partner of Fzr in Drosophila. The localization of Fzr to the centriole during interphase depends on direct interaction with Spd2. By generating Spd2 mutants unable to bind Fzr, we show that centrosomal localization of Fzr is essential for optimal APC/C activation towards its centrosomal substrate Aurora A. Finally, we show that Spd2 is also a novel APC/C(Fzr) substrate. Our study is the first to demonstrate the critical importance of distinct subcellular pools of APC/C activators in the spatiotemporal control of APC/C activity.Cancer Research UK (Career Development Fellowship), Biotechnology and Biological Sciences Research Council (project grant), Medical Research Council (project grant), Japan Society for the Promotion of Science (Postdoctoral Fellowship for Research Abroad), European Commission (Marie Skłodowska-Curie actions individual fellowship)This is the final version of the article. It first appeared from Nature Publishing Group via http://dx.doi.org/10.1038/ncomms1260

The conserved C2 phospholipid‐binding domain in Delta contributes to robust Notch signalling

Abstract: Accurate Notch signalling is critical for development and homeostasis. Fine‐tuning of Notch–ligand interactions has substantial impact on signalling outputs. Recent structural studies have identified a conserved N‐terminal C2 domain in human Notch ligands which confers phospholipid binding in vitro. Here, we show that Drosophila ligands Delta and Serrate adopt the same C2 domain structure with analogous variations in the loop regions, including the so‐called β1‐2 loop that is involved in phospholipid binding. Mutations in the β1‐2 loop of the Delta C2 domain retain Notch binding but have impaired ability to interact with phospholipids in vitro. To investigate its role in vivo, we deleted five residues within the β1‐2 loop of endogenous Delta. Strikingly, this change compromises ligand function. The modified Delta enhances phenotypes produced by Delta loss‐of‐function alleles and suppresses that of Notch alleles. As the modified protein is present on the cell surface in normal amounts, these results argue that C2 domain phospholipid binding is necessary for robust signalling in vivo fine‐tuning the balance of trans and cis ligand–receptor interactions

The role of Sgt1 in cell division

Ciências BiomédicasBiomedical Science

Uma guerra sem trégua : o combate ao jogo do bicho em Porto Alegre no alvorecer do século XX

O trabalho intitulado "'Uma guerra sem trégua': o combate ao jogo do bicho em Porto Alegre no alvorecer do século XX" pretendeu reconstruir a partir das fontes policiais, judiciais e da bibliografia pertinente os inúmeros expedientes utilizados pelo Poder Público para acabar com a prática do "jogo do bicho". Utilizando-se de um mercado lotérico já existente, tal modalidade de jogo alcançou enorme sucesso entre os porto-alegrenses. Este jogo foi capaz de congregar elementos de uma tradição onírica antiguíssima às rápidas mudanças que passavam a sociedade do período, sendo percebido pelos moralistas como mais um dos tantos sinais do despreparo da população local para a vida "civilizada".The paper titled "'Uma guerra sem trégua': o combate ao jogo do bicho em Porto Alegre no alvorecer do século XX" tried to rebuild from the police and judicial sources and the literature relevant to many ways used by governmental agencies to end the practice of "jogo do bicho". Using a lottery market already available, such type of game has achieved great success among the portoalegrenses. This game was able to bring together elements of an old tradition of the interpretation of dreans to the rapid changes that society went through at that period and being perceived by moralists as one of many signs that the local population was not prepared to live a "civilized" life