High thermal tolerance in high-elevation species and laboratory-reared colonies of tropical bumble bees

Bumble bees are key pollinators with some species reared in captivity at a commercial scale, but with significant evidence of population declines and with alarming predictions of substantial impacts under climate change scenarios. While studies on the thermal biology of temperate bumble bees are still limited, they are entirely absent from the tropics where the effects of climate change are expected to be greater. Herein, we test whether bees' thermal tolerance decreases with elevation and whether the stable optimal conditions used in laboratory-reared colonies reduces their thermal tolerance. We assessed changes in the lower (CTMin) and upper (CTMax) critical thermal limits of four species at two elevations (2600 and 3600 m) in the Colombian Andes, examined the effect of body size, and evaluated the thermal tolerance of wild-caught and laboratory-reared individuals of Bombus pauloensis. We also compiled information on bumble bees' thermal limits and assessed potential predictors for broadscale patterns of variation. We found that CTMin decreased with increasing elevation, while CTMax was similar between elevations. CTMax was slightly higher (0.84°C) in laboratory-reared than in wild-caught bees while CTMin was similar, and CTMin decreased with increasing body size while CTMax did not. Latitude is a good predictor for CTMin while annual mean temperature, maximum and minimum temperatures of the warmest and coldest months are good predictors for both CTMin and CTMax. The stronger response in CTMin with increasing elevation, and similar CTMax, supports Brett's heat-invariant hypothesis, which has been documented in other taxa. Andean bumble bees appear to be about as heat tolerant as those from temperate areas, suggesting that other aspects besides temperature (e.g., water balance) might be more determinant environmental factors for these species. Laboratory-reared colonies are adequate surrogates for addressing questions on thermal tolerance and global warming impacts

Top pair Asymmetries at Hadron colliders with general Z′Z' couplings

Recently it has been shown that measurement of charge asymmetry of top pair production at LHC excludes any flavor violating $Z'$ vector gauge boson that could explain Tevatron forward-backward asymmetry (FBA). We consider the general form of a $Z'$ gauge boson including left-handed, right-handed vector and tensor couplings to examine FBA and charge asymmetry. To evaluate top pair asymmetries at Tevatron and LHC, we consider $B^0_q$ mixing constraints on flavor changing $Z'$ couplings and show that this model still explain forward-backward asymmetry at Tevatron and charge asymmetry can not exclude it in part of parameters space.Comment: 18 pages, 7 figure

A Krüppel-like transcription factor gene is involved in salt stress responses in Medicago spp.

Legume plants are able to fix nitrogen in symbiotic association with rhizobia and, like many crops, are sensitive to high salt conditions. However, very few molecular markers can be associated to stress tolerance in legume crops. A Kruppel-like transcription factor, Mtzpt2-1, required for the formation of the nitrogen-fixing region, confers salt tolerance to yeast cells. Here, legume responses to salt stresses were studied using alfalfa and its close relative Medicago truncatula, a model legume species. Salt stress induces the Mszpt2-1 gene both in roots and root harbouring nodules. In addition, Sinorhizobium meliloti strains tolerating up to 700 mM NaCl, were used in nodulation assays to assess salt tolerance of the symbiotic response of M. truncatula. Few nodules, mainly in the upper part of the root, could be detected in plants treated with 200 mM NaCl, suggesting that nodule initiation was particularly sensitive to salt stress. We have also defined for M. truncatula the threshold of NaCl tolerance after which recovery of stressed plants is irreversible under laboratory conditions. After analysing several times of salt treatment (150 mM NaCl), M. truncatula 108R plants stressed for 7 days could not recover (less than 5%), whereas a 4-day treatment allowed at least 75% recovery. Transgenic M. truncatula plants expressing Mtzpt2-1 in antisense configuration are more sensitive to `recover' from salt stress than the wild type. These results identify Mtzpt2-1 as a molecular marker potentially linked to stress tolerance in M. truncatula and suggest its participation in a transcriptional program induced in these plants to cope with salt stress.Facultad de Ciencias ExactasInstituto de Biotecnología y Biología Molecula

A Replicative In Vitro Assay for Drug Discovery against Leishmania donovani.

The protozoan parasite Leishmania donovani is the causative agent of visceral leishmaniasis, a disease potentially fatal if not treated. Current available treatments have major limitations, and new and safer drugs are urgently needed. In recent years, advances in high-throughput screening technologies have enabled the screening of millions of compounds to identify new antileishmanial agents. However, most of the compounds identified in vitro did not translate their activities when tested in in vivo models, highlighting the need to develop more predictive in vitro assays. In the present work, we describe the development of a robust replicative, high-content, in vitro intracellular L. donovani assay. Horse serum was included in the assay media to replace standard fetal bovine serum, to completely eliminate the extracellular parasites derived from the infection process. A novel phenotypic in vitro infection model has been developed, complemented with the identification of the proliferation of intracellular amastigotes measured by EdU incorporation. In vitro and in vivo results for miltefosine, amphotericin B, and the selected compound 1 have been included to validate the assay

Measurement of the gluon PDF at small x with neutrino telescopes

We analyze the possibility that neutrino telescopes may provide an experimental determination of the slope lambda of the gluon distribution in the proton at momentum fractions x smaller than the accelerator reach. The method is based on a linear relation between lambda and the spectral index (slope) of the down-going atmospheric muon flux above 100 TeV, for which there is no background. Considering the uncertainties in the charm production cross section and in the cosmic ray composition, we estimate the error on the measurement of lambda through this method, excluding the experimental error of the telescopes, to be ~ +/- 0.2Comment: 16 pages with 16 figures - new version, comments added, same results and figure

Light MSSM Higgs boson mass to three-loop accuracy

The light CP even Higgs boson mass, Mh, is calculated to three-loop accuracy within the Minimal Supersymmetric Standard Model (MSSM). The result is expressed in terms of DRbar parameters and implemented in the computer program H3m. The calculation is based on the proper approximations and their combination in various regions of the parameter space. The three-loop effects to Mh are typically of the order of a few hundred MeV and opposite in sign to the two-loop corrections. The remaining theory uncertainty due to higher order perturbative corrections is estimated to be less than 1 GeV.Comment: 39 pages, 13 figures. v2: minor changes, typos fixe

PTt history from kyanite-sillimanite migmatites and garnet-staurolite schists from the Bayankhongor area, Mongolia indicates suprasubduction switching from extension to compression during Rodinia assembly

The tectonometamorphic evolution of the peri-Siberian tract of the Central Asian Orogenic Belt is mainly characterized by Baikalian Late Proterozoic - Early Cambrian cycle related to amalgamation of Proterozoic oceanic and continent fragments to Siberain landmass. Here we present in-situ monazite geochronology linked to P−T modelling of micashischsts and migmatite gneisses at the northern part of the Precambrian Baydrag block (central Mongolia) previously considered as a part of Baikalian metamorphic belt. Garnet-sillimanite-kyanite gneiss records first burial to the sillimanite stability at ~725 °C and 6.5 kbar, followed by burial to the kyanite stability at ~650 °C and ~8 kbar. The garnet-staurolite schist records burial to the staurolite-stability at ~620 °C and 6 kbar, followed by a nearly isothermal burial to ~580 °C and 9 kbar. The monazite data yield a continuum of 207Pb-corrected 238U/206Pb dates of c. 926−768 Ma in the Grt−Sil−Ky gneiss, and c. 937−754 Ma in the Grt-St schist. Based on monazite textural positon and internal zoning, the time of prograde burial and peak under a thermal gradient of 28-32 °C/km is estimated at c. 870−890 Ma. It is not clear whether such high grade conditions prevailed until a phase of further burial under a geothermal gradient of 18-22 °C/km and dated at 800−820 Ma. Additionally, monazite with dates of c. 568−515 Ma occurs as whole grains or as rims with sharp boundaries on Grenvillean monazite in Grt-St schist testifying for minor Baikalian overprint. Metamorphic zircon rims with Th/U ratio ~0.01-0.06 in Grt−Sil−Ky gneiss with 877 ± 7 Ma age, together with lower intercepts of zircon discordia lines in both Grt-Sil-Ky gneiss and Grt-St schist further support the Tonian age of high grade metamorphism. The P−T and geochronology data show anticlockwise P−T evolution from c. 930 to 750 Ma which is interpreted as a result of thickening of suprasubduction extensional and hot edifice - probably of back arc or arc type. This kind of prograde metamorphism was so far described only on the northern part of the Tarim block and interpreted as a result of initiation of peri-Rodinian subduction of Mirovoi Ocean. Here, we further discuss geodynamic consequences of a unique discovery of Tonian metamorphism in term of tectonic switch related to initiation of peri-Rodinian oceanic subduction during supercontinent assembly followed by strong mechanical coupling potentially related to onset of Rodinia splitting

Lepton Fluxes from Atmospheric Charm

We reexamine the charm contribution to atmospheric lepton fluxes in the context of perturbative QCD. We include next-to-leading order corrections and discuss theoretical uncertainties due to the extrapolations of the gluon distributions at small-x. We show that the charm contribution to the atmospheric muon flux becomes dominant over the conventional contribution from pion and kaon decays at energies of about 10^5 GeV. We compare our fluxes with previous calculations.Comment: 19 pages, latex, revtex, psfi

A Search for Mid-Infrared Molecular Hydrogen Emission from Protoplanetary Disks

We observed the Herbig Ae/Be stars UX Ori, HD 34282, HD 100453, HD 101412, HD 104237 and HD 142666, and the T Tauri star HD 319139 and searched for H2 0-0 S(2) emission at 12.278 micron and H2 0-0 S(1) emission at 17.035 micron with VISIR, ESO-VLT's high-resolution MIR spectrograph. None of the sources present evidence for H2 emission. Stringent 3sigma upper limits to the integrated line fluxes and the mass of optically thin warm gas in the disks are derived. The disks contain less than a few tenths of Jupiter mass of optically thin H2 gas at 150 K at most, and less than a few Earth masses of optically thin H2 gas at 300 K and higher temperatures. We compare our results to a Chiang and Goldreich (1997, CG97) two-layer disk model. The upper limits to the disk's optically thin warm gas mass are smaller than the amount of warm gas in the interior layer of the disk, but they are much larger than the amount of molecular gas in the surface layer. We present a calculation of the expected thermal H2 emission from optically thick disks, assuming a CG97 disk structure, a gas-to-dust ratio of 100 and Tgas = Tdust. The expected H2 thermal emission fluxes from typical disks around Herbig Ae/Be stars (10^-16 to 10^-17 erg/s/cm2 at 140 pc) are much lower than the detection limits of our observations (5*10^-15 erg/s/cm2). H2 emission levels are very sensitive to departures from the thermal coupling between the molecular gas and dust. Additional sources of heating of gas in the disk's surface layer could have a major impact on the expected H2 disk emission. In the observed sources the molecular gas and dust in the surface layer have not significantly departed from thermal coupling (Tgas/Tdust< 2) and that the gas-to-dust ratio in the surface layer is very likely lower than 1000.Comment: 16 pages, 9 figures, accepted by A&A. v2: typo in footnote ** corrected, v3: corrections of the A&A language editor included, typo in title of Fig. 1. correcte

Scalar-mediated ttˉt\bar t forward-backward asymmetry

A large forward-backward asymmetry in $t\bar t$ production, for large invariant mass of the $t\bar t$ system, has been recently observed by the CDF collaboration. Among the scalar mediated mechanisms that can explain such a large asymmetry, only the t-channel exchange of a color-singlet weak-doublet scalar is consistent with both differential and integrated $t\bar t$ cross section measurements. Constraints from flavor changing processes dictate a very specific structure for the Yukawa couplings of such a new scalar. No sizable deviation in the differential or integrated $t\bar t$ production cross section is expected at the LHC.Comment: 22 pages, 1 figure and 2 tables. v2: Corrected Eqs.(50,51,74), adapted Fig.1, Tab.1 and relevant discussions. Extended discussion of top decay and single to