Ceramic materials lead to underestimated DNA quantifications : a method for reliable measurements

In the context of investigating cell-material interactions or of material-guided generation of tissues, DNA quantification represents an elective method to precisely assess the number of cells attached or embedded within different substrates. Nonetheless, nucleic acids are known to electrostatically bind to ceramics, a class of materials commonly employed in orthopaedic implants and bone tissue engineering scaffolds. This phenomenon is expected to lead to a relevant underestimation of the DNA amount, resulting in erroneous experimental readouts. The present work aims at *lpar;i) investigating the effects of DNA-ceramic bond occurrence on DNA quantification, and (ii) developing a method to reliably extract and accurately quantify DNA in ceramic-containing specimens. A cell-free model was adopted to study DNA-ceramic binding, highlighting an evident DNA loss (up to 90%) over a wide range of DNA/ceramic ratios (w/w). A phosphate buffer-based (800 mM) enzymatic extraction protocol was developed and its efficacy in terms of reliable DNA extraction and measurement was confirmed with commonly used fluorometric assays, for various ceramic substrates. The proposed buffered DNA extraction technique was validated in a cell-based experiment showing 95% DNA retrieval in a cell seeding experiment, demonstrating a 3.5-fold increase in measured DNA amount as compared to a conventional enzymatic extraction protocol. In conclusion, the proposed phosphate buffer method consistently improves the DNA extraction process assuring unbiased analysis of samples and allowing accurate and sensitive cell number quantification on ceramic containing substrates

Analysis of broadband microwave conductivity and permittivity measurements of semiconducting materials

We perform broadband phase sensitive measurements of the reflection coefficient from 45 MHz up to 20 GHz employing a vector network analyzer with a 2.4 mm coaxial sensor which is terminated by the sample under test. While the material parameters (conductivity and permittivity) can be easily extracted from the obtained impedance data if the sample is metallic, no direct solution is possible if the material under investigation is an insulator. Focusing on doped semiconductors with largely varying conductivity, here we present a closed calibration and evaluation procedure for frequencies up to 5 GHz, based on the rigorous solution for the electromagnetic field distribution inside the sample combined with the variational principle; basically no limiting assumptions are necessary. A simple static model based on the electric current distribution proves to yield the same frequency dependence of the complex conductivity up to 1 GHz. After a critical discussion we apply the developed method to the hopping transport in Si:P at temperature down to 1 K.Comment: 9 pages, 10 figures, accepted for publication in the Journal of Applied Physic

Relativistic Coulomb Green's function in dd-dimensions

Using the operator method, the Green's functions of the Dirac and Klein-Gordon equations in the Coulomb potential $-Z\alpha/r$ are derived for the arbitrary space dimensionality $d$. Nonrelativistic and quasiclassical asymptotics of these Green's functions are considered in detail.Comment: 9 page

Accidental Father-to-Son HIV-1 Transmission During the Seroconversion Period

A 4-year-old child born to an HIV-1 seronegative mother was diagnosed with HIV-1, the main risk factor being transmission from the child's father who was seroconverting at the time of the child's birth. In the context of a forensic investigation, we aimed to identify the source of infection of the child and date of the transmission event. Samples were collected from the father and child at two time points about 4 years after the child's birth. Partial segments of three HIV-1 genes (gag, pol, and env) were sequenced and maximum likelihood (ML) and Bayesian methods were used to determine direction and estimate date of transmission. Neutralizing antibodies were determined using a single cycle assay. Bayesian trees displayed a paraphyletic-monophyletic topology in all three genomic regions, with the father's host label at the root, which is consistent with father-to-son transmission. ML trees found similar topologies in gag and pol and a monophyletic-monophyletic topology in env. Analysis of the time of the most recent common ancestor of each HIV-1 gene population indicated that the child was infected shortly after the father. Consistent with the infection history, both father and son developed broad and potent HIV-specific neutralizing antibody responses. In conclusion, the direction of transmission implicated the father as the source of transmission. Transmission occurred during the seroconversion period when the father was unaware of the infection and was likely accidental. This case shows how genetic, phylogenetic, and serological data can contribute for the forensic investigation of HIV transmission.info:eu-repo/semantics/publishedVersio

Engineering Time-Reversal Invariant Topological Insulators With Ultra-Cold Atoms

Topological insulators are a broad class of unconventional materials that are insulating in the interior but conduct along the edges. This edge transport is topologically protected and dissipationless. Until recently, all existing topological insulators, known as quantum Hall states, violated time-reversal symmetry. However, the discovery of the quantum spin Hall effect demonstrated the existence of novel topological states not rooted in time-reversal violations. Here, we lay out an experiment to realize time-reversal topological insulators in ultra-cold atomic gases subjected to synthetic gauge fields in the near-field of an atom-chip. In particular, we introduce a feasible scheme to engineer sharp boundaries where the "edge states" are localized. Besides, this multi-band system has a large parameter space exhibiting a variety of quantum phase transitions between topological and normal insulating phases. Due to their unprecedented controllability, cold-atom systems are ideally suited to realize topological states of matter and drive the development of topological quantum computing.Comment: 11 pages, 6 figure

Memory Effects and Scaling Laws in Slowly Driven Systems

This article deals with dynamical systems depending on a slowly varying parameter. We present several physical examples illustrating memory effects, such as metastability and hysteresis, which frequently appear in these systems. A mathematical theory is outlined, which allows to show existence of hysteresis cycles, and determine related scaling laws.Comment: 28 pages (AMS-LaTeX), 18 PS figure

In vivo functional and myeloarchitectonic mapping of human primary auditory areas

In contrast to vision, where retinotopic mapping alone can define areal borders, primary auditory areas such as A1 are best delineated by combining in vivo tonotopic mapping with postmortem cyto- or myeloarchitectonics from the same individual. We combined high-resolution (800 μm) quantitative T(1) mapping with phase-encoded tonotopic methods to map primary auditory areas (A1 and R) within the "auditory core" of human volunteers. We first quantitatively characterize the highly myelinated auditory core in terms of shape, area, cortical depth profile, and position, with our data showing considerable correspondence to postmortem myeloarchitectonic studies, both in cross-participant averages and in individuals. The core region contains two "mirror-image" tonotopic maps oriented along the same axis as observed in macaque and owl monkey. We suggest that these two maps within the core are the human analogs of primate auditory areas A1 and R. The core occupies a much smaller portion of tonotopically organized cortex on the superior temporal plane and gyrus than is generally supposed. The multimodal approach to defining the auditory core will facilitate investigations of structure-function relationships, comparative neuroanatomical studies, and promises new biomarkers for diagnosis and clinical studies

Three-dimensional theory of stimulated Raman scattering

We present a three-dimensional theory of stimulated Raman scattering (SRS) or superradiance. In particular we address how the spatial and temporal properties of the generated SRS beam, or Stokes beam, of radiation depends on the spatial properties of the gain medium. Maxwell equations for the Stokes field operators and of the atomic operators are solved analytically and a correlation function for the Stokes field is derived. In the analysis we identify a superradiating part of the Stokes radiation that exhibit beam characteristics. We show how the intensity in this beam builds up in time and at some point largely dominates the total Stokes radiation of the gain medium. We show how the SRS depends on geometric factors such as the Fresnel number and the optical depth, and that in fact these two factors are the only factors describing the coherent radiation.Comment: 21 pages 14 figure

A study of rotating globular clusters - the case of the old, metal-poor globular cluster NGC 4372

Aims: We present the first in-depth study of the kinematic properties and derive the structural parameters of NGC 4372 based on the fit of a Plummer profile and a rotating, physical model. We explore the link between internal rotation to different cluster properties and together with similar studies of more GCs, we put these in the context of globular cluster formation and evolution. Methods: We present radial velocities for 131 cluster member stars measured from high-resolution FLAMES/GIRAFFE observations. Their membership to the GC is additionally confirmed from precise metallicity estimates. Using this kinematic data set we build a velocity dispersion profile and a systemic rotation curve. Additionally, we obtain an elliptical number density profile of NGC 4372 based on optical images using a MCMC fitting algorithm. From this we derive the cluster's half-light radius and ellipticity as r_h=3.4'+/-0.04' and e=0.08+/-0.01. Finally, we give a physical interpretation of the observed morphological and kinematic properties of this GC by fitting an axisymmetric, differentially rotating, dynamical model. Results: Our results show that NGC 4372 has an unusually high ratio of rotation amplitude to velocity dispersion (1.2 vs. 4.5 km/s) for its metallicity. This, however, puts it in line with two other exceptional, very metal-poor GCs - M 15 and NGC 4590. We also find a mild flattening of NGC 4372 in the direction of its rotation. Given its old age, this suggests that the flattening is indeed caused by the systemic rotation rather than tidal interactions with the Galaxy. Additionally, we estimate the dynamical mass of the GC M_dyn=2.0+/-0.5 x 10^5 M_Sun based on the dynamical model, which constrains the mass-to-light ratio of NGC 4372 between 1.4 and 2.3 M_Sun/L_Sun, representative of an old, purely stellar population.Comment: Accepted for publication in A&A, 12 pages, 14 figures, 2 table