NSF GK-12 Urban Educators Program

poster abstractThe IUPUI NSF GK-12 Urban Educators program partners STEM graduate students with the K-12 community to bring science into the classroom. Our main goal and conceptual focus is to create an urban partnership between STEM graduate fellows, IPS STEM teachers, and IUPUI School of Science and IU School of Medicine faculty that centers on early exposure to field and laboratory research in biomedical and environmental science education, appropriate for grades 6-12. The largest activity of this project involves GK-12 Fellows working with STEM teachers to bring inquiry-based research projects, linked to classroom needs, and the Indiana Academic Standards, into classrooms. Fellows work under two multidisciplinary research themes: Medicine and Human Health and Discovering the Science of the Environment (DSE). The activities developed provide middle school and high school students with opportunities to learn science by engaging in on-going experimentation and fieldwork. This poster will present a series of posters from specific Fellow-Teacher teams, highlighting their accomplishments in bringing graduate level research into the K-12 classroom. Both Fellows and Teachers will be presenting their work and available to share their experiences and lessons learned

Introducing Biology Undergraduates to Authentic Research through Grand Challenges in Global Health: Examining Environmental Factors that Influence the Development of Zebrafish Embryos

To increase student excitement and engagement in science, a course-based undergraduate research experience (CURE) has been introduced in the curriculum at IUPUI. In Fall 2013, original research projects investigating prenatal alcohol, nicotine and caffeine exposure effects on development of zebrafish embryos was introduced into the Introductory Biology K102 course. This research project was also a part of a new Themed Learning Community (TLC) at IUPUI called “From Molecules to Medicines” that examined grand challenges in global health. In documenting the developmental effects on zebrafish embryos, and designing new protocols to address student research questions, students gained experience with authentic research methods, laboratory techniques, microscopy, image analyses, statistical analyses, scientific writing and presentation skills. This project, especially in a freshman undergraduate lab setting, requires a new way of problem-solving, but greatly facilitates student excitement and engagement in science through the use of research-based high-impact practices for student success and persistence. To continue an inquiry-based lab on global health issues and to keep IUPUI biology curricula current with the rapid rise of bioinformatics, concepts of bioinformatics were introduced into the Cell Biology Laboratory K325 course in Spring, 2014. Students were allowed to work on their own investigatory projects to analyzed zebrafish microarray data to find genes affected after ethanol exposure. Students used NCBI/ Ensembl databases to retrieve the gene/protein sequences, and various freely available tools (GeneBank, Protein Data Bank, BLAST, ClustalW, ExPasy, Phylogenetic Tree) to investigate the evolutionary conservation of genes/proteins affected after ethanol exposure. Student learnt 3D-protein structure construction and observed how 3D-protein structure could change with single amino acid changes. Preliminary assessment indicates that students are gaining an understanding the web-based databases and tools and enjoying the investigatory nature of the lab exercises

Using Zebrafish to implement a Course-Based Undergraduate Research Experience (CURE) to study Teratogenesis in Two Biology Laboratory Courses

poster abstractAbstract: Two related course-based undergraduate research experiences (CUREs) were introduced into a freshman introductory biology and a sophomore level cell biology class. In fall 2013, first semester freshman students were introduced to scientific research in an introductory biology laboratory course. Students were mentored to develop and execute original research projects investigating embryonic nicotine and caffeine exposure effects on development, particularly on heart development and function, using zebrafish embryos. In spring 2014, sophomore level cell biology students extended these studies and analyzed the effects of nicotine and caffeine at precise times in gastrulation. The freshman research experience was repeated in fall 2014 where a new group of students expanded the earlier research to investigate effects of additional toxicants on development. Students designed new protocols, made measurements, documented data, presented results and generated novel, high quality preliminary data that will be further studied in successive semesters. Student researchers identified novel effects of nicotine exposure on gastrulation and heart morphogenesis. Student surveys showed the greatest gains in ability to (1) design experiments, (2) analyze data, and (3) make scientific presentations. This CURE approach generated excitement and engagement that translated into high student satisfaction and enhanced learning

Using Amino Acid Derivatives to Inhibit Pseudomonas aeruginosa Biofilm Formation on Cystic Fibrosis Bronchial Epithelia Cells

poster abstractCystic Fibrosis is a genetic disease caused by a mutation which inhibits the proper transport of sodium and chloride ions across epithelium. Improper ion transport results in the accumulation of thick mucus in critical organs such as the lungs, pancreas, liver, and intestines. The genetic mutation is incurable, but treating the symptoms can vastly increase life expectancy. CF patients are often afflicted with bacterial infections which colonize the excess mucus within the lungs. The most prevalent pathogen associated with CF lung infection is Pseudomonas aeruginosa, a Gram-negative bacterium found in soil and water. Pseudomonas aeruginosa exists in two forms: planktonic (free-swimming) and sessile (immobile within a biofilm community). The planktonic form is about 1,000x more susceptible to antibiotics and immune cells than the sessile form. Biofilm communities of sessile bacteria are protected by an exopolysaccharide layer outside of the cell wall. Small molecules which inhibit biofilm formation or initiate biofilm disassembly can dramatically increase the effectiveness of drugs and the immune system. In order to identify novel biofilm-inhibitory molecules, we assessed the activity of a library of small molecules in biofilm assays. Active compounds were then screened for activity on living Cystic Fibrosis bronchial epithelial cells infected with Pseudomonas aeruginosa. Compounds which successfully inhibit biofilm formation without affecting the Cystic Fibrosis bronchial epithelium cells can potentially be a new drug for treating Cystic Fibrosis infections

Effect of Curcuminoids in Turmeric on Developing Zebrafish Treated with Ethanol

poster abstractThis experiment was designed with the intention of determining whether turmeric could act as a rescue agent to prevent or mitigate the extent of Fetal Alcohol Spectrum Disorder (FASD) caused by early ethanol exposure using zebrafish as a model system. A range of turmeric concentrations were made from a stock solution of turmeric dissolved in ethanol (1mg turmeric in 5mL ethanol). The active agents in turmeric are the curcuminoids: Curcumin, Desmethoxycurcumin, and Bisdemethoxycurcumin. The curcuminoids concentration was estimated using liquid chromatography. These agents were present in the turmeric stock solution at the following concentrations: Bisdemethoxycurcumin: 36.6 +/- 0.1 ug/mL, Desmethoxycurcumin: 43.4 +/- 0.1 ug/mL, and Curcumin: 124.1 +/- 0.2 ug/mL. Untreated zebrafish embryos were placed in embryo medium, ethanol treated embryos in 100mM ethanol containing embryo medium, and turmeric co-supplemented medium with differing concentrations of turmeric. Since the turmeric stock solution was dissolved in ethanol, the concentration of ethanol was kept at a constant 100mM ethanol and the amount of turmeric solution added. The concentrations of the test plates were then based on this solution and made to be 100 mM ethanol and 1.16 uM curcuminoids, 100 mM ethanol and 1.74 uM curcuminoids, and 100 mM ethanol and 2.32 uM curcuminoids. The developing embryos were treated with the turmeric solution and/or ethanol during 2-24 hours post fertilization (hpf). These embryos were imaged at 72 hpf and their body length and eye diameter were measured. The embryos supplemented with curcuminoids showed a significant rescue effect on the body length and eye diameter compared to ethanol treated embryos. This indicates that the curcuminoids acted as a rescue agent to reduce the effects that are typical of FASD in developing zebrafish

Turmeric Extract Rescues Ethanol‐Induced Developmental Defect in the Zebrafish Model for Fetal Alcohol Spectrum Disorder (FASD)

Prenatal ethanol exposure causes the most frequent preventable birth disorder, fetal alcohol spectrum disorder (FASD). The effect of turmeric extracts in rescuing an ethanol‐induced developmental defect using zebrafish as a model was determined. Ethanol‐induced oxidative stress is one of the major mechanisms underlying FASD. We hypothesize that antioxidant inducing properties of turmeric may alleviate ethanol‐induced defects. Curcuminoid content of the turmeric powder extract (5 mg/mL turmeric in ethanol) was determined by UPLC and found to contain Curcumin (124.1 ± 0.2 μg/mL), Desmethoxycurcumin (43.4 ± 0.1 μg/mL), and Bisdemethoxycurcumin (36.6 ± 0.1 μg/mL). Zebrafish embryos were treated with 100 mM (0.6% v/v) ethanol during gastrulation through organogenesis (2 to 48 h postfertilization (hpf)) and supplemented with turmeric extract to obtain total curcuminoid concentrations of 0, 1.16, 1.72, or 2.32 μM. Turmeric supplementation showed significant rescue of the body length at 72 hpf compared to ethanol‐treated embryos. The mechanism underlying the rescue remains to be determined

PONDWATER, BIOFILMS, AND CYSTIC FIBROSIS: INTRODUCING CUTTING EDGE RESEARCH INTO THE HIGH SCHOOL CLASSROOM

poster abstrac

Central Indiana STEM Talent Expansion Program: Student and Faculty Interventions

Funded by 5-year, $2M grant from the National Science Foundation, the Central Indiana STEM Talent Expansion Program (CI-STEP) at Indiana University-Purdue University Indianapolis (IUPUI) is creating a pipeline of students and a campus culture change to increase the number of undergraduates obtaining Science, Technology, Engineering, and Mathematics (STEM) degrees. CI-STEP addresses initiatives needed for transforming the undergraduate STEM experience by propagating, expanding, and creating new evidence-based educational innovations in undergraduate STEM education at IUPUI

The effects of implementing recitation activities on success rates in a college calculus course

Over a period of six years, three different types of recitation sessions were implemented into the large enrollment section of a college calculus course. During the fall semesters, the results on the departmental final examination, the DFW rates, and the one-year retention rates of students as STEM majors were examined by the type of recitation session used with the large enrollment section. The three types of recitation sessions studied were: (1) optional mentoring sessions at the Math Assistance Center conducted by undergraduate students (peer mentors), (2) required mentoring sessions conducted by graduate students, and (3) required VGNA (Verbal, Graphical or Geometric, Numeric, and Algebraic) Concept activities, which were also coupled with mentoring sessions conducted by graduate students. The success of the students in the large enrollment section of the course, which included one of the three different types of recitation sessions, was compared to the success of students in the small enrollment sections of the course (enrollments less than 50 students). The effects of using each type of recitation session on raising departmental final examination scores, lowering DFW rates, and raising one-year retention rates is presented. The results of this study demonstrate methods of raising student success rates in large enrollment (lecture-format) courses

Successful Integration of Distributed Drug Discovery (D3) Components: Computational, Synthetic, and Biological Evaluation of Phenylalanine Derivatives as Potential Biofilm Inhibitors

poster abstractDistributed Drug Discovery (D3) is a multidisciplinary approach to identifying molecules that exhibit activity in the treatment of neglected diseases such as malaria, leishmaniasis, and tuberculosis as well as recalcitrant cystic fibrosis (CF) airway infections. D3 seeks to accomplish this task by combining computational chemistry, synthetic chemistry, and biological screening all within an educational framework. Recent reports suggest that D-amino acids are effective in the disassembly and inhibition of bacterial biofilms, which are important for a number of bacterial infections, including those in the CF lung. Utilizing chemical drawing software, we constructed (enumerated) target phenylalanine derivatives from commercially available benzyl halides by substitution at the α position of an amino acid scaffold. A subset of these enumerated molecules was computationally selected for synthesis based on chemical properties. These compounds were synthesized using simple, solid-phase techniques in an undergraduate organic chemistry laboratory class. The resulting racemic unnatural amino acid derivatives were then screened for activity in a biofilm assay. The results show biofilm inhibition with synthesized phenylalanine derivatives. Analysis of the results reveals a trend between lipophilicity and the degree of biofilm inhibition. These new molecules may lead to an avenue for therapy for those CF individuals suffering with bacterial lung infection. As a part of the undergraduate curriculum, this work provides the first example of D3-linked undergraduate student computational analysis, synthesis, and biological evaluation