Publicar en otras revistas citando a la Revista Española de Nutrición Humana y Dietética puede ayudar a conseguir el Impact Factor: Thomson Reuters® nos observa [Editorial]

La publicación de artículos científicos en revistas con alto factor de impacto es uno de los parámetros que más se valora en la carrera de los investigadores. Aunque existen distintas instituciones que estiman diversos índices de repercusión, el único índice oficial y valorado de forma internacional es el Impact Factor (IF) de Thomson Reuters® que anualmente publica en el Journal Citation Reports (JCR). En este punto, es importante señalar que en estos momentos RENHyD está siendo observada por Thomson Reuters®, al haber sido incluida en un nuevo recurso de información de este productor, llamado ESCI (Emerging Sources Citation Index) y que es un nuevo índice de la colección principal de Web of ScienceT. Creemos que es necesario que todos los implicados en la revista (lectores e investigadores) sepan cómo pueden ayudar a que la revista crezca y mejore su repercusión, la única estrategia es citar los artículos publicados en RENHyD para fundamentar los nuevos trabajos que se publican en otras revistas con factor de impacto. El consumo de nuestras publicaciones es la estrategia que ayude a conseguir el factor de impacto

Genomic DNA isolation methods from honey bee (Apis mellifera L.) Spermatheca

The honey bee queen (Apis mellifera L.) has a polyandrous mating system, meaning that the queen mates on average with 17 drones from the surroundings in a congregation area. After the mating event, the spermatozoa of the drones are stored in an organ called spermatheca. Genetic analysis of the spermathecal content can provide an estimate of the genetic diversity and purity of the surrounding honey bee populations. This can be particularly useful for conservation and mating centers that need to monitor their populations’ genetic backgrounds. However, isolating enough DNA for genomic applications from such a small and complex matrix can be a challenge. Here, we compared the quantity and quality of DNA isolated using five methods: (i) phenol-chloroform-isopropanol, (ii) QIAamp DNA Minikit, (iii) QIAamp DNA Microkit, (iv) Macherey- Nagel Nucleospin Tissue, and (v) NEB Monarch Genomic DNA Purification Tissue. For each kit, when appropriate, variations including different isolation protocols, lysis incubation times, and the addition of RNA carrier were assayed. The quantity and quality of DNA extracted was assessed by spectrophotometric (SpectroStar®Nano LVis Plate) and fluorometric methods (Quantus ™ Fluorometer). Spectrophotometric quantification indicated nucleic acid concentrations ranging from 2.00 to 55.58 ng/μL, and in 91.43% of the cases, the A260/280 ratios were over 2.00, indicating an elevated presence of RNA. The fluorometric quantification, specific for double-stranded DNA, provided values ranging from 0.02 to 2.30 ng/μL. From the five methods, two alternative protocols of the commercial kit QIAamp DNA Microkit produced a sufficient DNA quantity (≥1.7 ng/μL measured by Quantus) for applications involving SNP genotyping, namely: the Tissue protocol with 6 hours of lysis incubation and the Tissue protocol with 3 hours of incubation, both with addition of RNA carrier. In contrast, overnight lysis decreased the DNA yield. The other methods generally produced low and/or inconsistent DNA recovery. According to our results, QIAamp DNA Microkit with the use of RNA carrier and lysis incubation times between 3 to 6 hours produce the required DNA quantities for SNP genotyping.Contributions of JW and EM were financed through the financial support of the German Federal Ministry for Food and Agriculture, through the intermediary of the Federal Office for Agriculture and Food (project no. 2818BM040).info:eu-repo/semantics/publishedVersio

Transcriptome innovations in primates revealed by single-molecule long-read sequencing

Transcriptomic diversity greatly contributes to the fundamentals of disease, lineage-specific biology, and environmental adaptation. However, much of the actual isoform repertoire contributing to shaping primate evolution remains unknown. Here, we combined deep long- and short-read sequencing complemented with mass spectrometry proteomics in a panel of lymphoblastoid cell lines (LCLs) from human, three other great apes, and rhesus macaque, producing the largest full-length isoform catalog in primates to date. Around half of the captured isoforms are not annotated in their reference genomes, significantly expanding the gene models in primates. Furthermore, our comparative analyses unveil hundreds of transcriptomic innovations and isoform usage changes related to immune function and immunological disorders. The confluence of these evolutionary innovations with signals of positive selection and their limited impact in the proteome points to changes in alternative splicing in genes involved in immune response as an important target of recent regulatory divergence in primates

ASPECTOS DO MANEJO DA VERMINOSE OVINA NA REGIÃO OESTE DE SANTA CATARINA

Os ovinos foram uma das primeiras espécies de animais a serem domesticados pelo homem, estando hoje presentes em praticamente todos os continentes. A ampla distribuição dos ovinos em diversos ambientes deve-se em grande parte à sua alta capacidade de adaptação aos diferentes climas e pastagens. Essa característica pode ser observada dentro do próprio território brasileiro, onde a ovinocultura é explorada em diversos estados, tanto com fins lucrativos, quanto para subsistência de famílias em áreas rurais (VIANA, 2008).A ovinocultura brasileira vem apresentando um crescimento constante. Isto se deve ao aumento no consumo da carne ovina e à viabilização desse sistema de produção em áreas impróprias para outras explorações pecuárias ou mesmo para a implantação de lavouras (NICIURA; VERÍSSIMO; MOLENTO, 2009)

Suplementación con magnesio y control metabólico en diabetes. Revisión sistemática de revisiones sistemáticas y metaanálisis

VIII Congreso Iberoamericano de Nutrición. ¿Nutrición basada en la videncia o en la evidencia

Aplicaciones móviles para la pérdida de peso: Revisión sistemática de ensayos clínicos controlados

VIII Congreso Iberoamericano de Nutrición. ¿Nutrición basada en la videncia o en la evidencia

Toma de decisiones compartida e informada por las pruebas: la esencia de las recomendaciones nutricionales

VIII Congreso Iberoamericano de Nutrición. ¿Nutrición basada en la videncia o en la evidencia

Students 4 best RED-NuBE : una comunidad de aprendizaje de nutrición basada en la evidencia fundamentada en el trabajo colaborativo en red y de apoyo entre pares

II Congreso de Alimentación, Nutrición y Dietética. Avances en Nutrición y Dietética Clínica: Prevención, Tratamiento y Gestión - Rol del Dietista-Nutricionist

Students 4 best RED-NuBE : una comunidad de aprendizaje de nutrición basada en la evidencia fundamentada en el trabajo colaborativo en red y de apoyo entre pares

II Congreso de Alimentación, Nutrición y Dietética. Avances en Nutrición y Dietética Clínica: Prevención, Tratamiento y Gestión - Rol del Dietista-Nutricionist

Probing turbulent scattering effects on suprathermal electrons in the solar wind: modeling, observations, and implications

This study explores the impact of a turbulent scattering mechanism, akin to those influencing solar and galactic cosmic rays propagating in the interplanetary medium, on the population of suprathermal electrons in the solar wind. We employ a Fokker–Planck equation to model the radial evolution of electron pitch angle distributions under the action of magnetic focusing, which moves the electrons away from isotropy, and of a diffusion process that tends to bring them back to it. We compare the steady-state solutions of this Fokker–Planck equation with data obtained from the Solar Orbiter and Parker Solar Probe missions and find a remarkable agreement, varying the turbulent mean free path as the sole free parameter in our model. The obtained mean free paths are of the order of the astronomical unit, and display weak dependence on electron energy within the 100 eV–1 keV range. This value is notably lower than Coulomb collision estimates but aligns well with observed mean free paths of low-rigidity solar energetic particle events. The strong agreement between our model and observations leads us to conclude that the hypothesis of turbulent scattering at work on electrons at all heliospheric distances is justified. We discuss several implications, notably the existence of a low Knudsen number region at large distances from the Sun, which offers a natural explanation for the presence of an isotropic “halo” component at all distances from the Sun—electrons being isotropized in this distant region before traveling back into the inner part of the interplanetary medium