19 research outputs found
Appalachian Music: Discussing the Top Ten
Convenor Sandy Ballard, Editor of Appalachian Journal, Appalachian State University Moderators Mark Freed, Cultural Resources Coordinator for the town of Boone, NC, and teacher of Appalachian Music, Appalachian State University. Guest editor of Appalachian Music edition of Appalachian Journal. Trevor McKenzie, archivist, W. L. Eury Appalachian Collection, Appalachian State University. Guest editor of Appalachian Music edition of Appalachian Journal
Conventional and molecular assays aid diagnosis of crop diseases and fungicide resistance
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Temperature dependence of protein dynamics simulated with three different water models
The effect of variation of the water model on the temperature dependence of protein and hydration water dynamics is examined by performing molecular dynamics simulations of myoglobin with the TIP3P, TIP4P, and TIP5P water models and the CHARMM protein force field at temperatures between 20 and 300 K. The atomic mean-square displacements, solvent reorientational relaxation times, pair angular correlations between surface water molecules, and time-averaged structures of the protein are all found to be similar, and the protein dynamical transition is described almost indistinguishably for the three water potentials. The results provide evidence that for some purposes changing the water model in protein simulations without a loss of accuracy may be possible
EMSL Pore Scale Modeling Challenge/Workshop
Report covers the background for the workshop, objectives, important research directions, necessary capabilities and overall recommendations
Gas-phase H/D exchange and collision cross sections of hemoglobin monomers, dimers, and tetramers
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Insufficient spring irrigation increases abnormal splitting of pistachio nuts
Abnormal pistachio nuts, known as “early-split nuts,” have both the hull and shell split while still on the tree, exposing the kernel to invasion by insects and molds. Deficit irrigation of pistachio trees in April and May resulted in substantial increases in the formation of early-split nuts in late summer, while extreme deficit irrigation in July and August resulted in decreased formation. However, deficit irrigation during the period of shell hardening, mainly in June, did not affect early-split formation
Ochratoxin Production by the Aspergillus ochraceus Group and Aspergillus alliaceus
Ochratoxin A is a toxic and carcinogenic fungal secondary metabolite; its presence in foods is increasingly regulated. Various fungi are known to produce ochratoxins, but it is not known which species produce ochratoxins consistently and which species cause ochratoxin contamination of various crops. We isolated fungi in the Aspergillus ochraceus group (section Circumdati) and Aspergillus alliaceus from tree nut orchards, nuts, and figs in California. A total of 72 isolates were grown in potato dextrose broth and yeast extract-sucrose broth for 10 days at 30°C and tested for production of ochratoxin A in vitro by high-pressure liquid chromatography. Among isolates from California figs, tree nuts, and orchards, A. ochraceus and Aspergillus melleus were the most common species. No field isolates of A. ochraceus or A. melleus produced ochratoxin A above the level of detection (0.01 μg/ml). All A. alliaceus isolates produced ochratoxin A, up to 30 μg/ml. We examined 50,000 figs for fungal infections and measured ochratoxin content in figs with visible fungal colonies. Pooled figs infected with A. alliaceus contained ochratoxin A, figs infected with the A. ochraceus group had little or none, and figs infected with Penicillium had none. These results suggest that the little-known species A. alliaceus is an important ochratoxin-producing fungus in California and that it may be responsible for the ochratoxin contamination occasionally observed in figs
Conventional and molecular assays aid diagnosis of crop diseases and fungicide resistance
For the past decade, we have been developing techniques for monitoring pre- and postharvest diseases of tree fruit, nuts and vines at the UC Kearney Research and Extension Center (KREC). We have also advanced new methods to monitor pathogen resistance to fungicides, which growers can now use to make decisions on disease management. Although accurate, the conventional techniques are time-consuming and only provide results after 5 to 21 days. Molecular methods offer the possibility of faster, more reliable and efficient tests. We have developed such methods to monitor, diagnose and quantify crop pathogens. We have also used these new molecular techniques to answer complex questions on the biology of tree fruit and nut pathogens, the origin of their inoculum sources, changes in their population structures and the epidemiology of the diseases they cause. We are now working on molecular techniques that could supplement the conventional ones we have developed for vine diseases. Private diagnostic laboratories have been using a number of these conventional techniques and plan to adopt some of the molecular ones in the near future