ATIVIDADE ANTIMICROBIANA DO MEL DE ABELHAS JATAÍ (Tetragonisca angustula) EM CULTIVO DE MICRORGANISMOS DO CONDUTO AUDITIVO DE CANINOS DOMÉSTICOS (Canis familiaris)

The present work aimed to investigate, in vitro, the efficacyof the honey of Tetragonisca angustula, against the microorganismsfound in the auditory canal inflammations of dogs. The sampleswere collected through a sterile swab of secretion of the auditorycanal of dogs with clinical otitis. The material was fixed in glassslide by performing the smear, Gram stained, and submittedto microscopic identification. The samples were inoculated inTryptone Soya Broth culture media and incubated at 37°C for24 hours. After verifying that there was growth, the sampleswere cultured in Petri’s plates containing Chapman, Levine andMueller-Hinton, and incubated at 37°C for 24 hours. Filter paperdiscs were adapted, sterilized in autoclave and embedded in honeyfor the antibiogram. The disks impregnated with honey wereplaced in Petri’s plates containing the contamined material andagain incubated at 37°C for 24 hours. This experiment verified theformation of inhibition halos in the antibiograma with the honey ofthe Tetragonisca angustula, showing to be effective in the controlof Staphylococcus sp, Bacillus sp and yeasts