Evolution of Neuropeptide Precursors in Polyneoptera (Insecta)

Neuropeptides are one of the major players in regulation of growth, reproduction and development as well as in information transfer in all multicellular animal organisms (Metazoa). Structurally, these short protein-like substances are among the most diverse signaling molecules, although they are under a stabilizing evolutionary pressure due to co-evolution with their respective receptor. Many of the peptidergic systems have a very ancient origin that can be traced back to the early evolution of Metazoa. In arthropods, especially insects, neuropeptide sequences and functions have been intensely studied in the past. In recent years, the increasing availability of genome and transcriptome data facilitated the investigation of complete neuropeptide precursor sequences and aided in the discovery of new neuropeptide genes. In my thesis, mainly transcriptome data were used to study the evolution of neuropeptide precursor sequences of polyneopteran insects, which resulted in three publications. In my dissertation, I could generate valuable insight into the evolution of neuropeptide precursor sequences. Specifically, I was able to demonstrate that neuropeptide precursor sequence evolution does not follow a uniform rate. It is unique in every precursor. Albeit a rather high degree of sequence conservation in Polyneoptera, differences in evolutionary rate can be seen between orthologs of different lineages. Even on a single neuropeptide precursor, the degree of sequence conservation differs between the functional units. Furthermore, I was able to establish neuropeptide precursor sequences as a basis for phylogenetic analyses

A Draft of the Human Septin Interactome

Background: Septins belong to the GTPase superclass of proteins and have been functionally implicated in cytokinesis and the maintenance of cellular morphology. They are found in all eukaryotes, except in plants. In mammals, 14 septins have been described that can be divided into four groups. It has been shown that mammalian septins can engage in homo- and heterooligomeric assemblies, in the form of filaments, which have as a basic unit a hetero-trimeric core. In addition, it has been speculated that the septin filaments may serve as scaffolds for the recruitment of additional proteins. Methodology/Principal Findings: Here, we performed yeast two-hybrid screens with human septins 1-10, which include representatives of all four septin groups. Among the interactors detected, we found predominantly other septins, confirming the tendency of septins to engage in the formation of homo- and heteropolymeric filaments. Conclusions/Significance: If we take as reference the reported arrangement of the septins 2, 6 and 7 within the heterofilament, (7-6-2-2-6-7), we note that the majority of the observed interactions respect the ""group rule"", i.e. members of the same group (e. g. 6, 8, 10 and 11) can replace each other in the specific position along the heterofilament. Septins of the SEPT6 group preferentially interacted with septins of the SEPT2 group (p<0.001), SEPT3 group (p<0.001) and SEPT7 group (p<0.001). SEPT2 type septins preferentially interacted with septins of the SEPT6 group (p<0.001) aside from being the only septin group which interacted with members of its own group. Finally, septins of the SEPT3 group interacted preferentially with septins of the SEPT7 group (p<0.001). Furthermore, we found non-septin interactors which can be functionally attributed to a variety of different cellular activities, including: ubiquitin/sumoylation cycles, microtubular transport and motor activities, cell division and the cell cycle, cell motility, protein phosphorylation/signaling, endocytosis, and apoptosis.Fundao de Amparo a Pesquisa do Estado Sao Paulo (Fapesp)CAPES: Coordenao de Aperfeioamento de Pessoal de Navel SuperiorConselho Nacional de Pesquisa e Desenvolvimento (CNPq)Laboratorio Nacional de Biociencias-Centro Nacional de Pesquisa em Energia e Materais (LNBio-CNPEM

Two new fossil genera and species of Cerocephalinae (Hymenoptera, Chalcidoidea, Pteromalidae), including the first record from the Eocene

Cerocephalinae (Chalcidoidea, Pteromalidae) is a small group of parasitoid wasps characterized by a number of derived diagnostic features. Their hosts are endophytic beetles. So far, 43 species of Cerocephalinae have been described, including one fossil species from the Miocene. In this study, we add two new genera and species from Baltic and Dominican amber to the fossil record. Tenuicornus dominicus gen. et sp. n. is the second genus described from Dominican amber, and Pteropilosa lailarabanorum gen. et sp. n., described from Baltic amber, represents the oldest record of the subfamily, pushing the minimum age of Cerocephalinae back to the Eocene. Diagnostic characters of both species are discussed in comparison with other Cerocephalinae. An updated key to extant and fossil Cerocephalinae is presented

Die Energiewende regional gestalten : auf dem Weg zu einer Energiewende-Roadmap im Ruhrgebiet

Diese Broschüre ist ein Produkt des Rahmenprogramms zur Umsetzung der Energiewende in den Kommunen des Ruhrgebiets: Die Auf­gabe des Projektes bestand darin auszuloten, in welcher Weise Kommunen ihre Handlungskapazitäten zur Umsetzung der Energiewende in der Region bewahren beziehungsweise perspektivisch erweitern können. Das Rahmenprogramm steuerte in seiner dreijährigen Projektlaufzeit (2013-2016) einen Beitrag zur Analyse der Handlungsfähigkeit von Kommunen im Ruhrgebiet zur Umsetzung der Energiewende bei und systematisierte bisherige Handlungsansätze in der Region. Es setzte an bestehenden Rahmenbedingungen an und versuchte, in Zusammenarbeit mit regionalen Akteurinnen und Akteuren künftige Handlungsansätze für eine langfristig angelegte Umsetzung der Energiewende in der Region zu identifizieren. Das vorliegende Dokument führt Ergebnisse des Pro­jektes zusammen und formuliert strategische Hinweise für die weitere Umsetzung der Energiewende im Ruhrgebiet