RDA e RIC-CM. Guardare oltre l’universo archivistico

Il contributo propone una breve riflessione sui punti di contatto e sulle divergenze tra lo standard RIC-CM e RD

Updated distribution of Osmoderma eremita in Abruzzo (Italy) and agro-pastoral practices affecting its conservation (Coleoptera: Scarabaeidae)

New records of Osmoderma eremita (Scopoli, 1763) (Coleoptera: Scarabaeidae: Cetoniinae) are reported for Abruzzo (Italy), together with a review of its distribution in this region. O. eremita is a saproxylic beetle dependent on the presence of hollow deciduous trees with abundant wood mould in their cavities. The major threats for the species are habitat loss and fragmentation. EU Habitats Directive requests to the member States its protection and the monitoring of its conservation status. Detection of its occurrence is the first step to protect the species. The surveys have been carried out in ten sites of Abruzzo by using black cross-windows traps baited with specific pheromone. The species has been recorded for the first time in the Sant'Antonio forest and its presence is confirmed in the Peligna Valley, after a decade. The populations seem to be confined to small patches of suitable habitats. At local level, the abandonment of the pollarding practice (willow and beech forests) and the use of pollarded trees as biomass for fuel are the major threats for this species. Indeed some key actions, such as the protection of old hollow trees and the continuation of pollarding practice in rural landscape, could be key factors for the conservation strategies of the species in the study area

High prevalence of anti-hepatitis e virus antibodies among blood donors in central Italy, february to march 2014

Prevalence of anti-hepatitis E virus (HEV) antibodies is highly variable in developed countries, which seems partly due to differences in assay sensitivity. Using validated sensitive assays, we tested 313 blood donors attending a hospital transfusion unit in central Italy in January and February 2014 for anti-HEV IgG and IgM and HEV RNA. Data on HEV exposure were collected from all donors. Overall anti-HEV IgG prevalence was 49% (153/313). Eating raw dried pig-liver sausage was the only independent predictor of HEV infection (adjusted prevalence rate ratio = 2.14; 95% confidence interval: 1.23–3.74). Three donors were positive for either anti-HEV IgM (n = 2; 0.6%) or HEV RNA (n = 2; 0.6%); they were completely asymptomatic, without alanine aminotransferase (ALT) abnormalities. Of the two HEV RNA-positive donors (both harbouring genotype 3), one was anti-HEV IgG- and IgM-positive, the other was anti-HEV IgG- and IgM-negative. The third donor was positive for anti-HEV IgG and IgM but HEV RNA-negative. HEV infection is therefore hyperendemic among blood donors (80% men 18–64 years-old) from central Italy and associated with local dietary habits. Nearly 1% of donors have acute or recent infection, implying potential transmission to blood recipients. Neither ALT nor anti-HEV IgM testing seems useful to prevent transfusion-transmitted HEV infection. © 2016, European Centre for Disease Prevention and Control

Limb reconstruction with knee mega-prosthesis in patients with distal femur primary tumours: gait analysis and alignment evaluation

Introduction: The aim of this study was the functional evaluation and lower limb alignment assessment of patients with a modular knee prosthesis after distal femur resection for primary bone tumour. Materials and methods: 15 patients affected by distal femur tumor and treated with a megaprosthesis implant (6 females and 9 males, mean age: 41 years, range: 15-74 years) and 15 controls were recruited for the study. For each subject the function evaluation included an instrumented gait analysis, while only patients underwent a teleradiography and a latero-lateral X-ray projection of the knee. Results: The duration of the stance phase of gait was shortened in the prosthetic limb with respect to the contralateral limb (57.5±3.6 % gait cycle vs. 60.9±4.8 % gait cycle, P = 0.01), with a correspondent increase of the swing phase. The prosthetic limb also showed an altered knee joint kinematic curve during gait, with a flexion deficit at load response with respect to the contralateral limb (4.5±3.6° vs. 13.4±5.0°, P = 0.000003). Abnormal timing in the muscle activation intervals were observed for tibialis anterior, gastrocnemius lateralis and rectus femoris of the prosthetic limb. The prosthetic limb was found to be misaligned with respect to the contralateral limb, both for the femorotibial (P<0.05) and the ankle joints (P<0.05). Conclusions: Gait analysis performed widely in reference centres could lead to a change in the design of megaprostheses to improve the function and prevent degenerative changes in not involved joint. A multicentric expertise is mandator

Time-lapsing biodiversity: an open source method for measuring diversity changes by remote sensing

Understanding biodiversity changes in time is crucial to promptly provide management practices against diversity loss. This is overall true when considering global scales, since human-induced global change is expected to make significant changes on the Earth's biota. Biodiversity management and planning is mainly based on field observations related to community diversity, considering different taxa. However, such methods are time and cost demanding and do not allow in most cases to get temporal replicates. In this view, remote sensing can provide a wide data coverage in a short period of time. Recently, the use of Rao's Q diversity as a measure of spectral diversity has been proposed in order to explicitly take into account differences in a neighbourhood considering abundance and relative distance among pixels. The aim of this paper was to extend such a measure over the temporal dimension and to present an innovative approach to calculate remotely sensed temporal diversity. We demonstrated that temporal beta-diversity (spectral turnover) can be calculated pixel-wise in terms of both slope and coefficient of variation and further plotted over the whole matrix / image. From an ecological and operational point of view, for prioritisation practices in biodiversity protection, temporal variability could be beneficial in order to plan more efficient conservation practices starting from spectral diversity hotspots in space and time. In this paper, we delivered a highly reproducible approach to calculate spatio-temporal diversity in a robust and straightforward manner. Since it is based on open source code, we expect that our method will be further used by several researchers and landscape managers

Contributo alla flora del Parco Nazionale della Majella

Viene presentato un ulteriore contributo alla ricca flora del Parco Nazionale della Majella. In particolare, 15 nuove entità vengono segnalate per la prima volta ed una viene confermata nell’area protetta. Per altre entità, rare sul territorio regionale, vengono segnalate nuove stazioni all’interno del Parco. Il contributo conferma come la ricerca sul campo sia di fondamentale importanza per approfondire le conoscenze sul patrimonio naturale, anche nel caso di territori abbastanza ben conosciuti come quello del Parco Nazionale della Majella.A further contribution to the rich flora of the Majella National Park is presented. In particular, 15 entities are reported for the first time and one is confirmed in the protected area. New localities are also indicated, inside the Park, for some plant species that are very rare in the Abruzzo region. It is confirmed that field research is of crucial importance in expanding our knowledge of the natural heritage, even in a fairly well known territory as that of the Majella National Park

Genotyping and antibiotic resistance traits in Campylobacter jejuni and coli from pigs and wild boars in Italy

The present study investigated the genomic constitution and antimicrobial resistance (AMR) of 238 Campylobacter from pigs and wild boars in Italy between 2012 and 2019. Campylobacter strains were genotyped using multilocus sequence typing (MLST) and whole genome MLST (wgMLST), screened for antimicrobial resistance genes, and tested for phenotypic susceptibility to six different antibiotics. C. coli was detected in 98.31% and 91.66% of pigs and wild boars, while C. jejuni was isolated in the remaining cases. MLST assigned 73 STs and 13 STs in pigs and wild boars, respectively, including 44 novel STs. The predominant ST in pigs was ST-854 (12.36%), followed by ST-9264 (6.18%). ST-1055 and ST-1417 were predominant in wild boars (30% and 13.33%, respectively). The minimum spanning tree using 1,121 global MLST profiles showed specific Italian clusters and a clear separation between pig and wild boar profiles. The wgMLST confirmed the MLST clustering and revealed a high genetic diversity within C. coli population in Italy. Minimum inhibitory concentrations (MIC) of six antibiotics revealed higher resistance in pigs to ciprofloxacin, nalidixic acid, streptomycin and tetracycline, compared to wild boar. In contrast, most strains were susceptible to gentamicin. Worrying levels of multidrug resistance (MDR) were observed mostly in pig isolates. Molecular screening of AMR mechanisms revealed the predominance of gyrA T86I substitution among fluoroquinolone- and quinolone-resistant isolates, and the 23S rRNA A2075G mutation among macrolide-resistant isolates. Other resistance determinants were observed: (i) tet(O) gene was present among tetracycline-resistant isolates; (ii) rpsL and aph(3’)-III genes conferring resistance to aminoglycosides, were identified only in streptomycin or gentamicin-resistant pig isolates; (iii) cmeA, cmeB, cmeC, cmeR genes responsible of pump efflux mechanisms, were observed in almost all the strains; (iv) OXA-61, encoding β-lactamase, was found in the half of the strains. Genotypic and phenotypic AMR profiling was fairly correlated for quinolones/fluoroquinolones. Campylobacter infection is common also in wild boar populations in Italy, suggesting that wild boars could be a reservoir of resistant and multi-resistant Campylobacter species, which may be of public health concern. The present study adds to our knowledge on the epidemiological and ecological traits of this pathogen in domesticated and wild swine

The influence of tethered epidermal growth factor on connective tissue progenitor colony formation

Strategies to combine aspirated marrow cells with scaffolds to treat connective tissue defects are gaining increasing clinical attention and use. In situations such as large defects where initial survival and proliferation of transplanted connective tissue progenitors (CTPs) are limiting, therapeutic outcomes might be improved by using the scaffold to deliver growth factors that promote the early stages of cell function in the graft. Signaling by the epidermal growth factor receptor (EGFR) plays a role in cell survival and has been implicated in bone development and homeostasis. Providing epidermal growth factor (EGF) in a scaffold-tethered format may sustain local delivery and shift EGFR signaling to pro-survival modes compared to soluble ligand. We therefore examined the effect of tethered EGF on osteogenic colony formation from human bone marrow aspirates in the context of three different adhesion environments using a total of 39 donors. We found that tethered EGF, but not soluble EGF, increased the numbers of colonies formed regardless of adhesion background, and that tethered EGF did not impair early stages of osteogenic differentiation.National Institute of General Medical Sciences (U.S.) (Grant NIH RO1 AR42997)National Institute of General Medical Sciences (U.S.) (Grant NIH RO1 AG024980)National Institute of General Medical Sciences (U.S.) (Grant NIH RO1 GM59870)National Institute of General Medical Sciences (U.S.) (Grant NIH DE019523

Antimicrobial resistance genotypes and phenotypes of Campylobacter jejuni isolated in Italy from humans, birds from wild and urban habitats, and poultry

Campylobacter jejuni, a common foodborne zoonotic pathogen, causes gastroenteritis worldwide and is increasingly resistant to antibiotics. We aimed to investigate the antimicrobial resistance (AMR) genotypes of C. jejuni isolated from humans, poultry and birds from wild and urban Italian habitats to identify correlations between phenotypic and genotypic AMR in the isolates. Altogether, 644 C. jejuni isolates from humans (51), poultry (526) and wild- and urban-habitat birds (67) were analysed. The resistance phenotypes of the isolates were determined using the microdilution method with EUCAST breakpoints, and AMR-associated genes and single nucleotide polymorphisms were obtained from a publicly available database. Antimicrobial susceptibility testing showed that C. jejuni isolates from poultry and humans were highly resistant to ciprofloxacin (85.55% and 76.47%, respectively), nalidixic acid (75.48% and 74.51%, respectively) and tetracycline (67.87% and 49.02%, respectively). Fewer isolates from the wild- and urban-habitat birds were resistant to tetracycline (19.40%), fluoroquinolones (13.43%), and quinolone and streptomycin (10.45%). We retrieved seven AMR genes (tet (O), cmeA, cmeB, cmeC, cmeR, blaOXA-61 and blaOXA- 184) and gyrA-associated point mutations. Two major B-lactam genes called blaOXA-61 and blaOXA-184 were prevalent at 62.93% and 82.08% in the poultry and the other bird groups, respectively. Strong correlations between genotypic and phenotypic resistance were found for fluoroquinolones and tetracycline. Compared with the farmed chickens, the incidence of AMR in the C. jejuni isolates from the other bird groups was low, confirming that the food-production birds are much more exposed to antimicrobials. The improper and overuse of antibiotics in the human population and in animal husbandry has resulted in an increase in antibiotic-resistant infections, particularly fluoroquinolone resistant ones. Better understanding of the AMR mechanisms in C. jejuni is necessary to develop new strategies for improving AMR programs and provide the most appropriate therapies to human and veterinary populations