1,941 research outputs found
Occurrence of the Oribatid Mite Trhypochthoniellus longisetus longisetus (Acari: Trhypochthoniidae) on Tilapia (Oreochromis niloticus).
Mites as parasites infesting fish have been described in a few case reports involving Histiostoma anguillarum, H. papillata, and Schwiebea estradai. We describe the unexpected occurrence of oribatid mites of the genus Trhypochthoniellus on farmed tilapia Oreochromis niloticus. The fish had mites on the skin, fins, and gills, as well as in the mouth. The morphological characteristics of the mites, observed by optical and scanning electron microscopy, were consistent with those described for T. longisetus longisetus. All stages of development were observed, suggesting that the mites were able to actively reproduce on fis
Performance of beginning teachers in the licensure examination for teachers: a national study
The study explored the performances of teacher education graduates in the licensure examination for teachers (LET). The descriptive-comparative research was conducted through a survey questionnaire administered online to 2,780 beginning teachers across the 16 regions in the Philippines. The findings of this study revealed that the graduates in Elementary Education (BEEd) and Secondary Education (BSEd) had satisfactory LET performances from 2017 to 2019. The comparative analysis found that these LET performances significantly vary in their examination year, degree program, specializations, examination components, locale, and type of higher education institution. Furthermore, the performance of graduates in the LET examination could be attributed to their test-taking time, college preparation, and grade point average. Hence, the graduates’ satisfactory performances and significant impact on college preparation show that teacher education institutions have aligned their offerings to the standards set by the country’s higher education and licensure system. Strengthening curricular enhancement programs, reinforcing constructive alignment, and intensifying retention policy and mentoring practices were recommended
Synthesis of Fe-Au nanoparticles through phase separation using the gas aggregation technique
During the last veers different type of magnetic materials have been obtained either alloys or nanopor-ritlcs with severall metalllics shells. These particle exhibt better magnetic properties, are biocompatible and have optical properties due to their shell noble metal layer, this is possible, to synthesize heterostructured nanoparticles with care/shell structure by using sputtering targets consisting of alloys of different materials. In the case of such materials have different surface energies and atomic sizes, there are diffusion processes which lead to the formation of structured nanoparticles with a shell and core having different composition. In this work we will show the results obtained about Fe-Au nanoparticles grown by I he gas aggregation technique, using magnetron sputering sources. Colloids prepared from sputtered deposits of heterostructed nanoparticles exhibit less aggregation when compared to suspensions obtained from pure magnetic materials. Spectrophotometry measurement show the presence of gold at the surface of the nanoparticles. Magnetic properties of such particle are analyzed by VSM. Compasition end structural analysis are studied by TEM and ÉDA
Maternal buffering beyond glucocorticoids: impact of early life stress on corticolimbic circuits that control infant responses to novelty
Maternal presence has a potent buffering effect on infant fear and stress responses in primates. We previously reported that maternal presence is not effective in buffering the endocrine stress response in infant rhesus monkeys reared by maltreating mothers. We have also reported that maltreating mothers show low maternal responsiveness and permissiveness/secure-base behavior. Although still not understood, it is possible that this maternal buffering effect is mediated, at least partially, through deactivation of amygdala response circuits when mothers are present. Here we studied rhesus monkey infants that differed in the quality of early maternal care to investigate how this early experience modulated maternal buffering effects on behavioral responses to novelty during the weaning period. We also examined the relationship between these behavioral responses and structural connectivity in one of the underlying regulatory neural circuits: amygdala-prefrontal pathways. Our findings suggest that infant exploration in a novel situation is predicted by maternal responsiveness and structural integrity of amygdala-prefrontal white matter depending on maternal presence (positive relationships when mother is absent). These results provide evidence that maternal buffering of infant behavioral inhibition is dependent on the quality of maternal care and structural connectivity of neural pathways that are sensitive to early life stress
UNC-Emory Infant Atlases for Macaque Brain Image Analysis: Postnatal Brain Development through 12 Months
Computational anatomical atlases have shown to be of immense value in neuroimaging as they provide age appropriate reference spaces alongside ancillary anatomical information for automated analysis such as subcortical structural definitions, cortical parcellations or white fiber tract regions. Standard workflows in neuroimaging necessitate such atlases to be appropriately selected for the subject population of interest. This is especially of importance in early postnatal brain development, where rapid changes in brain shape and appearance render neuroimaging workflows sensitive to the appropriate atlas choice. We present here a set of novel computation atlases for structural MRI and Diffusion Tensor Imaging as crucial resource for the analysis of MRI data from non-human primate rhesus monkey (Macaca mulatta) data in early postnatal brain development. Forty socially-housed infant macaques were scanned longitudinally at ages 2 weeks, 3, 6, and 12 months in order to create cross-sectional structural and DTI atlases via unbiased atlas building at each of these ages. Probabilistic spatial prior definitions for the major tissue classes were trained on each atlas with expert manual segmentations. In this article we present the development and use of these atlases with publicly available tools, as well as the atlases themselves, which are publicly disseminated to the scientific community
Early maternal rejection affects the development of monoaminergic systems and adult abusive parenting in rhesus macaques (Macaca mulatta).
Early Life Exposure to Unpredictable Parental Sensory Signals Shapes Cognitive Development Across Three Species
Exposure to early life adversity has long term consequences on cognitive function. Most research has focused on understanding components of early life adversities that contribute to later risk, including poverty, trauma, maltreatment, and neglect. Whereas these factors, in the aggregate, explain a significant proportion of emotional and cognitive problems, there are serious gaps in our ability to identify potential mechanisms by which early life adversities might promote vulnerability or resilience. Here we discuss early life exposure to unpredictable signals from the caretaker as an understudied type of adversity that is amenable to prevention and intervention. We employ a translational approach to discover underlying neurobiological mechanisms by which early life exposure to unpredictable signals sculpts the developing brain. First, we review evidence that exposure to unpredictable signals from the parent during sensitive periods impacts development of neural circuits. Second, we describe a method for characterizing early life patterns of sensory signals across species. Third, we present published and original data illustrating that patterns of maternal care predict memory function in humans, non-human primates, and rodents. Finally, implications are discussed for identifying individuals at risk so that early preventive-intervention can be provided
Electrochemical characterization of biodeterioration of paint films containing cadmium yellow pigment
[EN] The voltammetry of microparticles (VMP) methodology was used to characterize the biological attack of different bacteria and fungi to reconstructed egg tempera and egg linseed oil emulsion paint films containing cadmium yellow (CdS), which mimic historical painting techniques. When these paint films are in contact with aqueous acetate buffer, different cathodic signals are observed. As a result of the crossing of VMP data with attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), scanning electrochemical microscopy (SECM), field emission scanning electron microscopy (FESEM), and atomic force microscopy (AFM), these voltammetric signals can be associated with the reduction of CdS and different complexes associated to the proteinaceous and fatty acid fractions of the binders. After biological attack with different fungi (Acremonium chrysogenum, Aspergillus niger, Mucor rouxii, Penicillium chrysogenum, and Trichoderma pseudokoningii) and bacteria (Arthrobacter oxydans, Bacillus amyloliquefaciens, and Streptomyces cellulofans), the observed electrochemical signals experience specific modifications depending on the binder and the biological agent, allowing for an electrochemical monitoring of biological attack.Financial support from the MINECO Projects CTQ2014-53736-C3-1-P and CTQ2014-53736-C3-2-P which are supported with ERDF funds is gratefully acknowledged. The authors also wish to thank Dr. José Luis Moya López, Mr. Manuel Planes Insausti, and Mrs. Alicia Nuez Inbernón (Microscopy Service of the Universitat Politècnica de València) for technical support.Ortiz-Miranda, A.; Domenech Carbo, A.; Domenech Carbo, MT.; Osete Cortina, L.; Valle-Algarra, FM.; Bolivar Galiano, F.; Martin Sanchez, I.... (2016). Electrochemical characterization of biodeterioration of paint films containing cadmium yellow pigment. Journal of Solid State Electrochemistry. 20(12):3287-3302. https://doi.org/10.1007/s10008-016-3349-6S328733022012Ratledge C (1994) Biochemistry of microbial degradation. Springer, BerlinCaneva G, Nugari MP, Salvadori O (2008) Plant biology for cultural heritage, the Getty Conservation Institute, Los AngelesSterflinger K (2010) Fungi: their role in deterioration of cultural heritage. Fungal Biol Rev 47–55 and references thereinGargani G (1968) Fungus contamination of Florence art masterpieces before and after the 1966 disaster. In: Walters AH, Elphick JJ (eds) Biodeterioration of materials. Elsevier, Amsterdam, pp. 252–257Seves AM, Sora S, Ciferr O (1996) The microbial colonization of oil paintings—a laboratory investigation. Int Biodeter Biodegr. 37:215–224Tiano P (2002) Biodegradation of cultural heritage: decay mechanisms and control methods. University of LisbonStrzelczyk AB (2004) Observations on aesthetic and structural changes induced in polish historic objects by microorganisms. Int Biodeter Biodegr. 53:151–156López-Miras M, Piñar G, Romero-Noguera J, Bolivar-Galiano FC, Ettenauer J, Sterflinger K, MartÃn-Sánchez I (2013) Microbial communities adhering to the obverse and reverse sides of an oil painting on canvas: identification and evaluation of their biodegradative potential. Aerobiologia 29:301–314Koszewski A, Rymuza Z, Reuther F (2008) Evaluation of nanomechanical, nanotribological and adhesive properties of ultrathin polymer resist film by AFM. Micro Engn 85:1189–1192Schabereiter-Gurtner C, Piñar G, Lubitz W, Rölleke S (2001) An advanced molecular strategy to identify bacterial communities on art objects. J Microbiol Meth 45:77–87Florian MLE (1996) The role of the conidia of fungi in fox spots. Stud Conserv 41:65–75Arai H, Matsui N, Matsumura N, Murakita H (1988) Biochemical investigations on the formation mechanisms of foxing. Stud Conserv 33:11–12Arai H, Matsumura N, Murakita H (1990) Microbiological studies on the conservation of paper and related cultural properties: part 9, induction of artificial foxing. Science for Conservation 29:25–34Hayashi T, Namili M (1986) Role of sugar fragmentation in early stage browning of amino-carbonyl reaction of sugars with amino acids. Agr Biol Chem Tokyo 50:1965–1970Allsopp D, Seal KJ, Gaylarde CC (2004) Introduction to biodeterioration, 2 edn. Cambridge University Press, CambridgeBock E, Sand W (1993) The microbiology of masonry biodeterioration. J Appl Bacteriol 74:503–514Ciferri O (2002) The role of microorganisms in the degradation of cultural heritage. Rev Conserv 3:35–45Van der Snickt G, Dik J, Cotte M, Janssens K, Jaroszewicz J, De Wolf W, Groenewegen J, Van der Loeff L (2009) Characterization of a degraded cadmium yellow (CdS) pigment in an oil painting by means of synchrotron radiation based X-ray techniques. Anal Chem 81:2600–2610Child AM (1995) Microbial taphonomy of archaeological bone. Stud Conserv 40:19–30Soliman NA, Knoll M, Abdel-Fattah YR, Schmid RD, Lange S (2007) Molecular cloning and characterization of thermostable esterase and lipase from Geobacillus thermoleovorans YN isolated from desert soil in Egypt. Process Biochem 42(2007):1090–1100Kinderlerer JL (1994) Degradation of the lauric acid oils. Int Biodeter Biodegr 33(1994):345–354van den Berg JDJ, van den Berg KJ, Boon JJ (2002) Identification of non-cross-linked compounds in methanolic extracts of cured and aged linseed oil-based paint films using gas chromatography-mass spectrometry. J Chromatogr A 950:195–211 and references thereinLefèvre M (1974) La ‘maladie verte’ de Lascaux. Stud Conserv 19:126–156Petushkova JP, Lyalikova NN (1986) Microbiological degradation of lead-containing pigments in mural paintings. Stud Conserv 31:65–69Breitbach AM, Rocha JC, Gaylarde CC (2011) Influence of pigment on biodeterioration of acrylic paint films in southern Brazil. J Coat Technol Res 8:619–628Keune K, van Loon A, Boon JJ (2011) SEM backscattered-electron images of paint cross sections as information source for the presence of the lead white pigment and lead-related degradation and migration phenomena in oil paintings. Microsc Microanal 17:696–701Meilunas RJ, Bentsen JG, Steinberg A (1990) Analysis of aged paint binders by FTIR spectroscopy. Stud Conserv 35:33–51Mazzeo R, Prati S, Quaranta M, Joseph E, Kendix E, Galeotti M (2008) Attenuated total reflection micro FTIR characterization of pigment–binder interaction in reconstructed paint films. Anal Bioanal Chem 392:65–76Salvadó N, Butà S, Nicholson J, Emerich H, Labrador A, Pradell T (2009) Identification of reaction compounds in micrometric layers from gothic paintings using combined SR-XRD and SR-FTIR. Talanta 79:419–428Scholz F, Meyer B (1998) Voltammetry of solid microparticles immobilized on electrode surfaces. Electroanal Chem 20:1–86Scholz F, Schröder U, Gulabowski R, Doménech-Carbó A (2014) Electrochemistry of immobilized particles and Dropletst, 2 edn. Springer, Berlin-HeidelbergDoménech-Carbó A, Labuda J, Scholz F (2013) Electroanalytical chemistry for the analysis of solids: characterization and classification (IUPAC technical report). Pure Appl Chem 85:609–631Doménech-Carbó A, Doménech-Carbó MT, Costa V (2009) Electrochemical methods for Archaeometry, conservation and restoration (monographs in electrochemistry series Scholz F edit). Springer, Berlin-HeidelbergDoménech-Carbó A (2010) Electrochemistry for conservation science. J Solid State Electr 14:349–351Matteini M, Moles A (1989) La Chimica nel Restauro. Nardini, FirenzeGettens RJ, Stout GL (1966) Painting materials. A short encyclopedia. Dover Publications, New YorkCennini C (1982) Il libro dell’ arte. Akal, MadridCepriá G, GarcÃa-Gareta E, Pérez-Arantegui J (2005) Cadmium yellow detection and quantification by voltammetry of immobilized microparticles. Electroanalysis 17:1078–1084DomÃnguez I, Doménech-Carbó A, Cerisuelo JP, López-Carballo G, Henández-Muñoz P, Gavara R (2014) Contact probe electrochemical characterization and metal speciation of silver LLDPE nanocomposite films. J Solid State Electrochem 18:2099–2110Byler DM, Susi H (1986) Examination of the secondary structure of proteins by deconvolved FTIR spectra. Biopolymers 25:469–487Chang CM, Powrie WD, Fennema O (1977) Microstructure of egg yolk. J Food Sci 42:1193–1200Prestrelski SJ, Tedeschi N, Arakawa T, Carpenter JF (1993) Dehydration-induced conformational transitions in proteins and their inhibition by stabilizers. Biophys J 65:661–671Boehm S, Abaturov LV (1977) Structural changes of met-haemoglobin by dehydration. FEBS Lett 77:21–24Karpowicz A (1981) Ageing and deterioration of proteinaceous media. Stud Conserv 26:153–160Koper A, Grabarczyk M (2012) Simultaneous voltammetric determination of trace bismuth(III) and cadmium(II) in water samples by adsorptive stripping voltammetry in the presence of cupferron. J Electroanal Chem 681:1–5Zakharchuk N, Meyer S, Lange B, Scholz F (2000) A comparative study of lead oxide modified graphite paste electrodes and solid graphite electrodes with mechanically immobilized lead oxides. Croat Chem Acta 73:667–704Komorsky-Lovric S, Lovric M, Bond AM (1992) Comparison of the square-wave stripping voltammetry of lead and mercury following their electrochemical or abrasive deposition onto a paraffin impregnated graphite electrode. Anal Chim Acta 258:299–305Arjmand F, Adriaens A (2012) Electrochemical quantification of copper-based alloys using voltammetry of microparticles: optimization of the experimental conditions. J Solid State Electrochem 16:535–543Meyer B, Ziemer B, Scholz F (1995) In situ X-ray diffraction study of the electrochemical reduction of tetragonal lead oxide and orthorhombic Pb(OH)Cl mechanically immobilized on a graphite electrode. J Electroanal Chem 392:79–83Hasse U, Scholz F (2001) In situ atomic force microscopy of the reduction of lead oxide nanocrystals immobilised on an electrode surface. Electrochem Commun 3:429–434Doménech-Carbó A, Doménech-Carbó MT, Mas-Barberá X (2007) Identification of lead pigments in nanosamples from ancient paintings and polychromed sculptures using voltammetry of nanoparticles/atomic force microscopy. Talanta 71:1569–1579Eissler RL, Princen RH (1972) The interface between reactive pigment and binder matrix. J Electroanal Chem 37:327–336Kuznetsov AM, Ulstrup J (1989) Protein dynamics and electronic fluctuation effects in electron transfer reactions of membrane-bound proteins and metalloprotein complexes. J Electroanal Chem 275:289–305Colletti LP, Teklay D, Stickney JL (1994) Thin-layer electrochemical studies of the oxidative underpotential deposition of sulfur and its application to the electrochemical atomic layer epitaxy deposition of CdS. J Electroanal Chem 369:145–152Gulaboski R, Mirceski V, Bogeski I, Hoth M (2012) Protein film voltammetry: electrochemical enzymatic spectroscopy. A review on recent progress. J Solid State Electrochem 16:2315–2328Guidelli R, Becucci L (2011) Ion transport across biomembranes and model membranes. J Solid State Electrochem 15:1459–1470Sutherland K (2003) Solvent-extractable components of linseed oil paint films. Stud Conserv 48:111–135Rossi M, Alamprese C, Ratti S (2007) Tocopherols and tocotrienols as free radical-scavengers in refined vegetable oils and their stability during deep-fat frying. Food Chem 102:812–817Ziyatdinova G, Morozov M, Budnikov H (2012) MWNT-modified electrodes for voltammetric determination of lipophilic vitamins. J Solid State Electrochem 16:2441–2447Madani A, Nessark B, Boukherroub R, Chehimi MM (2011) Preparation and electrochemical behaviour of PPy–CdS composite films. J Electroanal Chem 650:176–181Derrick MR, Stulik DC, Landry MJ (1999) Infrared spectroscopy in conservation science. Getty Conservation Institute, Los Angelesvan der Weerd J, van Loon A, Boon JJ (2005) FTIR studies of the effects of pigments on the aging of oil. Stud Conserv 50:3–22Kong J, Yu S (2007) Fourier transform infrared spectroscopic analysis of protein secondary structures. Acta Bioch Bioph Sin 39:549–559Haris PI, Severcan F (1999) FTIR spectroscopic characterization of protein structure in aqueous and non-aqueous media. J Mol Catal B-Enzym 7:207–221Furlan PY, Scott SA, Peaslee MH (2007) FTIR-ATR study of pH effects on egg albumin secondary structure. Spectrosc Lett 40:475–482Dong A, Huang P, Caughey WS (1990) Protein secondary structures in water from second-derivative amide I infrared spectra. Biochemistry-US 29:3303–3308Rajkhowa R, Hu X, Tsuzuki T, Kaplan DL, Wang X (2012) Structure and biodegradation mechanism of milled B. mori silk particles. Biomacromolecules 13:2503–2512Anton M (2013) Egg yolk: structures, functionalities and processes. J Sci Food Agr 93:2871–2880Hevonoja T, Pentikäinen MO, Hyvönen MT, Kovanen PT, Ala-Korpela M (2000) Structure of low density lipoprotein (LDL) particles: basis for understanding molecular changes in modified LDL. Biochim Biophys Acta 1488:189–210Kumpula LS, Kumpula JM, Taskinen MR, Jauhiainen M, Kaski K, Ala-Korpela M (2008) Reconsideration of hydrophobic lipid distributions in lipoprotein particles. Chem Phys Lipids 155:57–62 and references thereinSchneider H, Morrod RS, Colvin JR, Tattrie NH (1973) The lipid core model of lipoproteins. Chem Phys Lipids 10:328–353Doménech-Carbó MT, Osete-Cortina L, de la Cruz-Cañizares J, BolÃvar-Galiano F, Romero-Noguera J, MartÃn-Sánchez I, Fernández-Vivas MA (2006) Study of the microbiodegradation of terpenoid resin-based varnishes from easel painting using pirolisis-gas chromatography-mass spectrometry and gas chromatography-mass spectrometry. Anal Bioanal Chem 385:1265–128
Clinical features and outcomes of COVID-19 in patients with rheumatic diseases treated with biological and synthetic targeted therapies
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