Evaluación de los procesos de preparación de superficie con chorro abrasivo y aplicación de recubrimientos en espacios confinados de embarcaciones con miras hacia la implementación de un sistema que mejore la eficiencia de la división de pinturas en la empresa Cotecmar

La división de pinturas de la corporación de ciencia y tecnología COTECMAR tiene como actividades principales en el área de reparaciones la preparación de superficie y aplicación de recubrimientos, entre otros servicios generales a todos los tipos de embarcaciones marítimas y fluviales que llegan a la empresa con la necesidad de alguno de los servicios anteriormente mencionados, para llevar a cabo todos los procesos anteriores se vale de procedimientos específicos, determinados y validados por la división. Una preparación adecuada de la superficie, garantiza que un buen recubrimiento pueda mantenerse en buenas condiciones por un largo periodo de tiempo, la rutina de preparación se realiza en el casco, superestructura y cubiertas de la embarcación así como en su interior (tanques, sentinas, pañoles). La división de pinturas de acuerdo con las normas internacionales establecidas para la preparación de superficies metálicas usa la técnica blasting, sin embargo, según el grado de limpieza que se desea obtener, el área a trabajar y la exigencia del cliente la técnica puede variar y para esto la división cuenta con otros tipos de procedimientos como rutina mecánica y piqueteoIncluye bibliografía, anexo

Smartphones, foto-elicitación y desarrollo profesional docente en la transformación digital de universidades

La mirada del alumnado universitario a la práctica docente a través de su Smartphone es la protagonista de la formación permanente de su profesorado. Concretamente, se analizan las motivaciones que les lleva a tomar fotografías, así como la funcionalidad asignada a sus móviles. Tanto las intenciones como las funciones no son visibles, son procesos mentales internos, íntimos, a los que hay que acceder con técnicas de corte cualitativo. Para ello, se ha realizado un estudio de caso múltiple. La foto-elicitación ha sido el procedimiento principal de recogida de información. Las categorías que contienen los resultados han emergido de los datos. Se concluye mostrando como motivación principal el registro de momentos de procesos de enseñanza-aprendizaje relacionados con los contenidos, o con las tareas, entre otros, que fueron algo especial, extraordinario, para el alumnado (atendiendo a su significado, a sensaciones de sorpresa o afecto) por tener interés para una evaluación formativa del profesorado. La portabilidad y otros elementos relacionados con la ergonomía del teléfono móvil han hecho posible darle una doble funcionalidad: crear o producir imágenes para representar ideas o sensaciones y, también, la de reproducirlas a través de la pantalla para poder analizar su contenido, reflexionar y debatir sobre el sentido y su relevancia en el desarrollo profesional docente y en el aprendizaje del alumnado. Ambas, soportadas en procesos de foto-elicitación y promotoras de situaciones innovadoras de formación, se han mostrado relevantes para una transformación digital universitaria de calidad.In the study reported in this article, the gaze of university students to the teaching practice through their Smartphone is the protagonist of the permanent training of their teachers. Specifically, the motivations that lead them to take photographs are analyzed, as well as the functionality assigned to their mobiles. Both the intentions and the functions are not visible, they are internal, intimate mental processes, which must be accessed with qualitative techniques. For this, a multiple case study has been carried out. Photo-elicitation has been the main information gathering procedure. The categories that contain the results have emerged from the data. It concludes by showing as the main motivation the recording of moments of teaching-learning processes related to the contents, or with the tasks, among others, that were something special, extraordinary, for the students (according to their meaning, feelings of surprise or affected). The photographed moments were important for the students to evaluate their teachers. Mobile phones have had a double function: creating images to represent ideas or sensations and, also, reproducing them through the screen to be able to analyze their content, reflect and debate on the meaning and its relevance in the professional development of teachers and in student learning. Both functions, supported by photo-elicitation processes and promoting innovative training situations, have been shown to be relevant for a quality university digital transformation

Peripheral and central fatigue after high intensity resistance circuit training

Introduction: The aim of this study was to investigate the effects of high intensity resistance circuit (HIRC) and traditional strength training (TST) on neuromuscular fatigue and metabolic responses.Methods: Twelve trained young subjects performed HIRC and TST in a counterbalanced order with 1 week rest in-between. The amount of workload and the inter-set time for each local muscle group were matched (180 s), however, the time between successive exercises differed. The twitch interpolation technique was used to test neuromuscular function of the knee extensor muscles. Blood lactate concentration was used to evaluate metabolic responses.Results: Maximum voluntary contraction and resting potentiated twitch amplitude (Qtw) were significantly reduced after HIRC, but there were not changes after TST, while reductions in voluntary activation were similar. Lactate concentration increased significantly more after HIRC.Conclusions: The higher lactate concentration after HIRC probably impaired excitation-contraction coupling, indicating larger peripheral fatigue than after TS

Análisis de la formación permanente del profesorado universitario mediante fotografías tomadas por su alumnado

This study explores whether university teaching can improve through the photographic materialization of students' perceptions during teaching situations. More specifically, we tapped into whether these photographs could help teachers evoke the content of their experiential images, thus generating doubts that led to any change in their teaching. The experiential, intimate and tacit nature of the data required an interpretative design based on a multiple case study. Data collection was done through photographs and photo-elicitation processes. The results obtained in a focus group, which were coded, showed that the students participated to assess the methodology followed in the subjects. Although their level of photographic literacy was different, they used large shots to show human interactions, and detail shots to focus on relevant elements. Following students’ assessment of lecturers’ work, the latter introduced some changes in their practice, especially for those aspects in which there was a discrepancy between their experiential perception and students’ perceptions. The low rate of change advises a review of the photo-elicitation procedure followed so that more dilemmas in relation to lecturers’ teaching practice can be brought up, thus inducing greater possibilities for change.Se estudia la mejora de la docencia universitaria mediante la materialización fotográfica de percepciones del alumnado durante situaciones de enseñanza. Se indaga si dichas fotografías ayudan al profesorado a evocar el contenido de sus imágenes experienciales y a generar dudas que lleven a algún cambio en su trabajo docente. La naturaleza experiencial, íntima y tácita de los datos requeridos para responder a los objetivos de conocimiento, exigió un diseño de corte interpretativo basado en un estudio de caso múltiple. La recogida de datos se hizo mediante fotografías y procesos de foto-elicitación. Los resultados obtenidos en un grupo de discusión, mediante codificaciones soportadas en datos, muestran que los estudiantes participaron para valorar la metodología seguida en las asignaturas. Aunque su nivel de alfabetización fotográfica era diferente, han usado el plano de conjunto, para mostrar las interacciones humanas, y el de detalle para enfocar elementos relevantes de la misma. El profesorado planteó algunos cambios en su práctica que son consecuencia de ciertas valoraciones que hicieron sus alumnos sobre su trabajo; concretamente, aquellas cuyo contenido era discrepante con sus imágenes experienciales. La baja tasa de cambios aconseja una revisión del procedimiento de foto-elicitación seguido, para promover dilemas docentes cuya resolución mejore su práctica de enseñanza

Smartphones, foto-elicitación y desarrollo profesional docente en la transformación digital de universidades

In the study reported in this article, the gaze of university students to the teaching practice through their Smartphone is the protagonist of the permanent training of their teachers. Specifically, the motivations that lead them to take photographs are analyzed, as well as the functionality assigned to their mobiles. Both the intentions and the functions are not visible, they are internal, intimate mental processes, which must be accessed with qualitative techniques. For this, a multiple case study has been carried out. Photo-elicitation has been the main information gathering procedure. The categories that contain the results have emerged from the data. It concludes by showing as the main motivation the recording of moments of teaching-learning processes related to the contents, or with the tasks, among others, that were something special, extraordinary, for the students (according to their meaning, feelings of surprise or affected). The photographed moments were important for the students to evaluate their teachers. Mobile phones have had a double function: creating images to represent ideas or sensations and, also, reproducing them through the screen to be able to analyze their content, reflect and debate on the meaning and its relevance in the professional development of teachers and in student learning. Both functions, supported by photo-elicitation processes and promoting innovative training situations, have been shown to be relevant for a quality university digital transformation.La mirada del alumnado universitario a la práctica docente a través de su Smartphone es la protagonista de la formación permanente de su profesorado. Concretamente, se analizan las motivaciones que les lleva a tomar fotografías, así como la funcionalidad asignada a sus móviles. Tanto las intenciones como las funciones no son visibles, son procesos mentales internos, íntimos, a los que hay que acceder con técnicas de corte cualitativo. Para ello, se ha realizado un estudio de caso múltiple. La foto-elicitación ha sido el procedimiento principal de recogida de información. Las categorías que contienen los resultados han emergido de los datos. Se concluye mostrando como motivación principal el registro de momentos de procesos de enseñanza-aprendizaje relacionados con los contenidos, o con las tareas, entre otros, que fueron algo especial, extraordinario, para el alumnado (atendiendo a su significado, a sensaciones de sorpresa o afecto) por tener interés para una evaluación formativa del profesorado. La portabilidad y otros elementos relacionados con la ergonomía del teléfono móvil han hecho posible darle una doble funcionalidad: crear o producir imágenes para representar ideas o sensaciones y, también, la de reproducirlas a través de la pantalla para poder analizar su contenido, reflexionar y debatir sobre el sentido y su relevancia en el desarrollo profesional docente y en el aprendizaje del alumnado. Ambas, soportadas en procesos de foto-elicitación y promotoras de situaciones innovadoras de formación, se han mostrado relevantes para una transformación digital universitaria de calidad

Low impact of different SNP panels from two building-loci pipelines on RAD-Seq population genomic metrics: case study on five diverse aquatic species

Información complementaria: https://doi.org/10.1186/s12864-021-07465-w.Background: The irruption of Next-generation sequencing (NGS) and restriction site-associated DNA sequencing (RAD-seq) in the last decade has led to the identification of thousands of molecular markers and their genotyping for refined genomic screening. This approach has been especially useful for non-model organisms with limited genomic resources. Many building-loci pipelines have been developed to obtain robust single nucleotide polymorphism (SNPs) genotyping datasets using a de novo RAD-seq approach, i.e. without reference genomes. Here, the performances of two building-loci pipelines, STACKS 2 and Meyer’s 2b-RAD v2.1 pipeline, were compared using a diverse set of aquatic species representing different genomic and/or population structure scenarios. Two bivalve species (Manila clam and common edible cockle) and three fish species (brown trout, silver catfish and small-spotted catshark) were studied. Four SNP panels were evaluated in each species to test both different building-loci pipelines and criteria for SNP selection. Furthermore, for Manila clam and brown trout, a reference genome approach was used as control. Results: Despite different outcomes were observed between pipelines and species with the diverse SNP calling and filtering steps tested, no remarkable differences were found on genetic diversity and differentiation within species with the SNP panels obtained with a de novo approach. The main differences were found in brown trout between the de novo and reference genome approaches. Genotyped vs missing data mismatches were the main genotyping difference detected between the two building-loci pipelines or between the de novo and reference genome comparisons. Conclusions: Tested building-loci pipelines for selection of SNP panels seem to have low influence on population genetics inference across the diverse case-study scenarios here studied. However, preliminary trials with different bioinformatic pipelines are suggested to evaluate their influence on population parameters according with the specific goals of each study

Low impact of different SNP panels from two building-loci pipelines on RAD-Seq population genomic metrics: case study on five diverse aquatic species

Background: The irruption of Next-generation sequencing (NGS) and restriction site-associated DNA sequencing (RAD-seq) in the last decade has led to the identification of thousands of molecular markers and their genotyping for refined genomic screening. This approach has been especially useful for non-model organisms with limited genomic resources. Many building-loci pipelines have been developed to obtain robust single nucleotide polymorphism (SNPs) genotyping datasets using a de novo RAD-seq approach, i.e. without reference genomes. Here, the performances of two building-loci pipelines, STACKS 2 and Meyer’s 2b-RAD v2.1 pipeline, were compared using a diverse set of aquatic species representing different genomic and/or population structure scenarios. Two bivalve species (Manila clam and common edible cockle) and three fish species (brown trout, silver catfish and small-spotted catshark) were studied. Four SNP panels were evaluated in each species to test both different building-loci pipelines and criteria for SNP selection. Furthermore, for Manila clam and brown trout, a reference genome approach was used as control. Results: Despite different outcomes were observed between pipelines and species with the diverse SNP calling and filtering steps tested, no remarkable differences were found on genetic diversity and differentiation within species with the SNP panels obtained with a de novo approach. The main differences were found in brown trout between the de novo and reference genome approaches. Genotyped vs missing data mismatches were the main genotyping difference detected between the two building-loci pipelines or between the de novo and reference genome comparisons. Conclusions: Tested building-loci pipelines for selection of SNP panels seem to have low influence on population genetics inference across the diverse case-study scenarios here studied. However, preliminary trials with different bioinformatic pipelines are suggested to evaluate their influence on population parameters according with the specific goals of each studyThe work undertaken in this project was funded by Xunta de Galicia Autonomous Government (GRC2014/010), Interreg Atlantic Area (Cockles project, EAPA_458/2016) and Girona University (MPCUdG2016/060) projects. Adrián Casanova was a Xunta de Galicia fellowship (ED481A-2017/091)S

Recurrent genetic variants and prioritization of variants of uncertain clinical significance associated with hereditary breast and ovarian cancer in families from the Region of Murcia

© 2023 the author(s). This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/. This document is the Published version of a Published Work that appeared in final form in Advances in Laboratory Medicine. To access the final edited and published work see https://doi.org/10.1515/almed-2023-0103Objectives Hereditary breast and ovarian cancer (HBOC) follows an autosomal dominant inheritance pattern of cancer susceptibility genes. The risk of developing this disease is primarily associated with germline mutations in the BRCA1 and BRCA2 genes. The advent of massive genetic sequencing technologies has expanded the mutational spectrum of this hereditary syndrome, thereby increasing the number of variants of uncertain clinical significance (VUS) detected by genetic testing. Methods A prevalence study of HBOC was performed within 2,928 families from the Region of Murcia, in southeastern Spain. Genetic testing enabled the identification of recurrent pathogenic variants and founder mutations, which were mainly related to the BRCA1 and BRCA2 genes. VUS testing was performed using a prioritization algorithm designed by our working group. Results Variants c.68_69del, c.212+1G>A, and c.5123C>A were detected in 30 % of BRCA1 carriers, whereas exon 2 deletion concurrent with c.3264dupT, c.3455T>G and c.9117G>A variants were found in 30 % of BRCA2 carriers. A total of 16 VUS (15 %) were prioritized. Conclusions The genotype-phenotype correlation observed in our study is consistent with the scientific literature. Furthermore, the founder effect of c.1918C>T (BRCA1) and c.8251_8254del (ATM) was verified in the Murcian population, whereas exon 2 deletion (BRCA2) was proven to be a Spanish founder mutation. Our algorithm enabled us to prioritize potentially pathogenic VUS that required further testing to determine their clinical significance and potential role in HBOC