Using a Robot to Treat Non-specific Low Back Pain: Results From a Two-Arm, Single-Blinded, Randomized Controlled Trial

Non-specific low back pain (NSLBP) affects many people and represents a high cost for health care. Manual pressure release of myofascial trigger points is used to treat NSLBP and is very effective but difficult to standardize since it is provided by different therapists, which also suffer musculoskeletal complications from this highly repetitive activity. A robot designed for this purpose may help in reducing these problems. Here, we present data from a two-arm, single-blinded, randomized controlled clinical trial evaluating the efficiency of a therapeutic massage robot (ADAMO) in reducing NSLBP (clinicaltrials.gov, registration number: NCT04882748). Forty-four patients were randomly distributed into the two arms of the study (robot vs. control). A physician filled the Oswestry disability index (ODI) before starting the treatment and at the end of it, in a blind fashion. In addition, patients filled a visual analogue scale (VAS) after each of the 10 treatment sessions. The ODI and the VAS were analyzed as the primary and secondary outcome measures. Both treatments (robot and control) resulted in a significantly lower ODI (p < 0.05). On the other hand, robot-treated patients significantly reduced their VAS levels (p = 0.0001) whereas control treatment did not reach statistical significance. Patients of both sexes obtained similar benefits from either treatment. Overweight patients (body mass index ≥ 25kg/m(2)) in the robot arm benefited more from the treatment (p = 0.008) than patients with normal weight. In conclusion, the ADAMO robot is, at least, as efficient as regular treatment in reducing low back pain, and may be more beneficial for specific patients, such as those with excessive weight

¡Qué monumento!: Una app de reconocimiento de imágenes para la difusión del patrimonio.

El presente TFM pretende desarrollar una aplicación de reconocimiento de imágenes para dispositivos móviles, denominada ¡Qué monumento!, con la finalidad de aportar al usuario información histórico-artística sobre la edificación objeto de su interés a través de una fotografía de la misma. Siguiendo el mismo carácter divulgativo, la empresa encargada de desarrollar esta aplicación diseñará también una serie de actividades complementarias, tales como concursos y juegos didácticos. Esta empresa quiere distinguirse por la defensa tanto del disfrute y crecimiento personal del visitante como del mantenimiento del espíritu de la ciudad visitada, generando un turismo de calidad, sostenible y respetuoso con el medio ambiente, unido a un uso responsable de las nuevas tecnologías, evitando el gasto de papel y fomentando el slow tourism y la preservación de las obras y espacios de la ciudad. This End-of-master work aims to develop an image recognition app for mobile devices, called ¡Qué monumento!, that provides the user with historical-artistic information about the building object of interest through a photograph of it. Following this informative purpose, the company in charge of developing this application will also design some complementary activities, such as contests and educational games. This company wants to be characterized by the defence of the enjoyment and personal growth of the visitor and the maintenance of the city’s spirit, generating tourism of quality, sustainable and respectful with the environment, together with a responsible use of the new technologies, avoiding paper wasting and encouraging slow tourism and the preservation of the city's monuments and spaces.<br /

Marble waste and pig slurry increment soil quality and reduce metal availability in a tailing pond

Tailing ponds pose environmental hazards, such as toxic metals reaching water sources through wind and water erosion and leaching. These abandoned mine sites contain materials with high contents of Fe- oxyhydroxides, sulfides, and heavy metals. As a consequence, soils have null vegetation and low soil organic matter. In this study, various physicochemical properties, together with microbial biomass carbon and available metals, were measured before and six months after application of marble waste (6.7 kg m-2), raw pig slurry (4.3 L m-2), and solid phase pig slurry (7 kg m-2) as reclamation strategy in a tailing pond in southeastern Spain, to reduce hazards for the environment and human health. Results showed that aggregate stability, pH, organic carbon, total nitrogen, cation exchange capacity, available phosphorus, exchangeable potassium and microbial biomass carbon increased with the application of the amendments, while available metals and metalloid (As, Cd, Pb and Zn) drastically decreased (90-99%). This study confirms the high effectiveness of initial applications of marble wastes together with pig slurry to initialize the recovery of ecosystems in bare mine soils under Mediterranean semiarid conditions.This work wasfunded by the European UnionLIFE+ project MIPOLARE (LIFE09 ENV/ES/000439). J.A. Acosta acknowledges a “Saavedra Fajardo” contract from Comunidad Autónoma de Murcia (Spain)

The Garden and Landscape as an Interdisciplinary Resource Between Experimental Science and Artistic-Musical Expression: Analysis of Competence Development in Student Teachers

The garden is a major educational resource that can be used for all areas of knowledge from an interdisciplinary perspective as it reflects the complexity and interactions of the natural environment. This research was carried out in the academic years 2017-2018 and 2018-2019 with 418 participants of from the second, third, and fourth year of the Degree in Primary Education at the University of Valencia. The use of the garden and the landscape is analyzed as a non-formal context for training primary school teachers through sensory experiences that contribute to the development of scientific and artistic competencies. The study focused on the relationship between natural sounds and emotions to promote the environmental awareness and active listening that are necessary to improve auditory perception and reflect on health problems caused by noise pollution. During the project, we discovered the synergies between experimental sciences and artistic-musical expression and their importance for developing university students' competencies in the professional, personal, and collective spheres. The activities consisted of (1) a walking tour from the Faculty of Education to the garden at 1 km from the center, to reflect on the origin and characteristics of sound, emotions and their relationship with music; (2) sensory experiences in the garden through visual and auditory interaction with the landscape; and (3) an immersion in quasi-real scenarios created by virtual reality. The analysis was carried out by means of an ad hoc questionnaire with Likert-type items focused on aspects of the usefulness of gardens and the activities involved in the general and specific skills of the degree and open questions for reflections on emotional health, environmental sustainability, and artistic-musical creation as a representation of the environment. The results show the importance of the garden as a resource for training of primary teachers in a holistic and global approach that contributes to the development of teaching skills and the acquisition of knowledge based on sensory experiences with the landscape

Targeted Metabolomics Shows That the Level of Glutamine, Kynurenine, Acyl-Carnitines and Lysophosphatidylcholines Is Significantly Increased in the Aqueous Humor of Glaucoma Patients.

The composition of the aqueous humor of patients with glaucoma is relevant to understand the underlying causes of the pathology. Information on the concentration of metabolites and small molecules in the aqueous humor of healthy subjects is limited. Among the causes of the limitations is the lack of healthy controls since, until recently, they were not surgically intervened; therefore, the aqueous humor of patients operated for cataract was used as a reference. Sixteen aqueous humor samples from healthy subjects undergoing refractive surgery and eight samples from glaucoma patients were used to assess the concentration of 188 compounds using chromatography and mass spectrometry. The concentration of 80 of the 188 was found to be reliable, allowing comparison of data from the two groups (glaucoma and control). The pattern found in the controls is similar to, but not the same as, that reported using samples from 'controls' undergoing cataract surgery. Comparing data from glaucoma patients and healthy subjects, 57 of the 80 compounds were significantly (p < 0.05) altered in the aqueous humor. Kynurenine and glutamine, but not glutamate, were significantly increased in the glaucoma samples. Furthermore, 10 compounds were selected considering a statistical score of p < 0.0001 and the degree of change of more than double or less than half. The level of C10 (decanoyl)-carnitine decreased, while the concentration of spermidine and various acyl-carnitines and lysophosphatidylcholines increased in glaucoma. Principal component analysis showed complete segregation of controls and cases using the data for the 10 selected compounds. The receiver operating characteristic curve these 10 compounds and for glutamine allowed finding cut-off values and significant sensitivity and specificity scores. The concentration of small metabolites in the aqueous humor of glaucoma patients is altered even when they take medication and are well controlled. The imbalance affects membrane components, especially those of the mitochondria, suggesting that mitochondrial abnormalities are a cause or consequence of glaucoma. The increase in glutamine in glaucoma is also relevant because it could be a means of keeping the concentration of glutamate under control, thus avoiding its potential to induce the death of neurons and retinal cells. Equally notable was the increase in kynurenine, which is essential in the metabolism of nicotine adenine dinucleotides

Especies bacterianas causantes de infección de vías urinarias en mujeres entre 15 a 80 años instrumentadas vesicalmente en los servicios de gineco obstetricia y medicina mujeres del Hospital Nacional General San Pedro, Usulután, período de julio a septiembre de 2011

Este trabajo de investigación tiene como objetivo identificar especies bacterianas causantes de infección de vías urinarias en mujeres entre 15 a 80 años instrumentadas vesicalmente en los servicios de Gineco-obstetricia y Medicina Mujeres del Hospital Nacional General San Pedro de Usulután en el período de julio a septiembre de 2011. La metodología empleada en la investigación es de tipo prospectivo, porque se basó en la situación actual de las infecciones de vías urinarias en mujeres que se les practicó el sondaje vesical, es de laboratorio, ya que se realizó el examen general de orina previo a la instrumentación y un urocultivo post retiro de sonda para identificar la presencia de especies bacterianas, es un tipo de población finita debido a que la cantidad de pacientes en éstos servicios es regular con un número estimado de 110 mujeres distribuidas en ambos servicios, tomándose la muestra a 78 pacientes, así también se implementaron criterios de inclusión que sirvieron para clasificar a las mujeres con cateterización urinaria, siendo uno de los criterios principales presentar el examen general de orina negativo, se utilizó la entrevista como técnica de trabajo de campo. De la muestra seleccionada 15 cultivos resultaron positivos a la presencia de especies bacterianas siendo Escherichia coli la que prevaleció, aislándola en 9 pacientes que fueron parte del estudio; el rango de edades más afectado se encontró entre 14-24 años, 25-35 años, debido a que las mujeres en éstas edades están más propensas a ser sometidas a éstos procedimientos. Por los resultados obtenidos que representan el 11.5% de cultivos positivos a Escherichia coli se dá por aceptada la hipótesis de investigación que dice: La especie bacteriana que causa infección de vías urinarias en mujeres entre 15 a 80 años instrumentadas vesicalmente en los servicios de Gineco- obstetricia y Medicina Mujeres del Hospital Nacional General San Pedro de Usulután es Escherichia coli con un porcentaje que excede el 3

Evaluation of various biomarkers for kidney monitoring during canine leishmaniosis treatment

Background: The objective of this study was to evaluate and compare the evolution of the profile currently recommended by the International Renal Interest Society (IRIS) (sCr, UPC and sSDMA) with a panel of other different kidney biomarkers during treatment for canine leishmaniosis. This panel included three urinary glomerular biomarkers (uIgG, uCRP and uferritin) and three urinary tubular biomarkers (uGGT, uNAG and uRBP). These biomarkers were measured in two groups of dogs with canine leishmaniosis at IRIS stage I. Group 1: dogs showing proteinuria (UPC > 0.5) before treatment which did not decrease after treatment; Group 2: dogs showing proteinuria before treatment which decreased after treatment. Results: Group 1 showed no significant changes in any biomarker after treatment. In group 2, among the biomarkers recommended by the IRIS, only UPC showed a significant decrease after treatment. However all biomarkers of glomerular damage showed a significant decrease after treatment, with uIgG/Cr and uCRP/Cr showing the greater decreases. In addition uRBP/Cr and uNAG/Cr showed significant decreases after treatment. Conclusions: In dogs with leishmaniosis at IRIS stage I that reduced UPC after treatment, there were no significant changes in serum creatinine and sSDMA. However, all the urine biomarkers evaluated with exception of uGGT showed a significant decrease. These decreases were more evident in those markers related with glomerular function, being uIgG/Cr the biomarker more associated with UPC. Further studies involving a larger number of animals and histological analysis of the kidney would be recommended to confirm these findings and evaluate the routine practical use of these urine biomarkers in canine leishmaniosis

Mechanical response of industrial concrete with SFRC and PFRC

Concrete is one of the most commonly used construction materials in the world due to its versatility. There are different types of concrete according to the required mechanical responses, and these will depend on the composition of the elements. Therefore, additional elements have been developed to improve the properties and conditions of concrete. One of these elements is reinforcing fibers made of steel, polypropylene, glass, and so on, which, according to the base material, geometry, and dosage, improve the mechanical and workability properties and decrease and/or prevent the generation of cracks, which are some of the most common problems in industrial slabs. This study performs an analysis of the changes in the mechanical properties of concrete (compressive strength, rupture modulus, modulus of elasticity, Poisson’s ratio, and residual stress) due to the addition of fiber-reinforced concrete (FRC) to determine the physical and mechanical conditions of the fibers that improve the concrete and its application in industrial concrete. Due to the large number of samples and variables, advanced statistical methods (analysis of variance and comparative index) were used in the numerical study, which allowed to analyze and compare several results at the same time. This research is divided into two stages. In the first stage, six steel fibers (with a dosage of 2.7, 6, and 11 and three of 28¿kg/m3) and five polypropylene fibers (with a dosage of 0.6, 2.15, and 2.7 and two of 3¿kg/m3) were used in the study, and compression and bending tests (ASTM C39 and C78, respectively) were performed on 35 cylinders and 45 beams. Improvements were identified in several fiber-reinforced concrete samples in terms of compressive strength: 67% of the steel fiber samples and 100% of the polypropylene fiber samples had values above the average value of the simple concrete; in terms of the modulus of rupture, 83% of the steel fiber samples and 80% of the polypropylene fiber samples had values above the average value of the simple concrete. In the second stage, one type of steel fiber and one type of polypropylene fiber were selected for a second mechanical analysis (64 cylinders, 72 beams, and 15 slabs) with dosages of 20, 30, and 40¿kg/m3 and 2.13, 4.25, and 6.38¿kg/m3, respectively. In the second stage, statistical analysis and modeling with nonlinear analysis were used to evaluate the results, where residual strength improved but Poisson’s ratio decreased when the dosage of fibers was increased.Peer ReviewedPostprint (published version

Unveiling the Metabolic changes on muscle cell metabolism underlying p-phenylenediamine toxicity

Rhabdomyolysis is a disorder characterized by acute damage of the sarcolemma of the skeletal muscle leading to release of potentially toxic muscle cell components into the circulation, most notably creatine phosphokinase (CK) and myoglobulin, and is frequently accompanied by myoglobinuria. In the present work, we evaluated the toxicity of p-phenylenediamine (PPD), a main component of hair dyes which is reported to induce rhabdomyolysis. We studied the metabolic effect of this compound in vivo with Wistar rats and in vitro with C2C12 muscle cells. To this aim we have combined multi-omic experimental measurements with computational approaches using model-driven methods. The integrative study presented here has unveiled the metabolic disorders associated to PPD exposure that may underlay the aberrant metabolism observed in rhabdomyolys disease. Animals treated with lower doses of PPD (10 and 20 mg/kg) showed depressed activity and myoglobinuria after 10 h of treatment. We measured the serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and creatine kinase (CK) in rats after 24, 48, and 72 h of PPD exposure. At all times, treatment with PPD at higher doses (40 and 60 mg/kg) showed an increase of AST and ALT, and also an increase of lactate dehydrogenase (LDH) and CK after 24 h. Blood packed cell volume and hemoglobin levels, as well as organs weight at 48 and 72 h, were also measured. No significant differences were observed in these parameters under any condition. PPD induce cell cycle arrest in S phase and apoptosis (40% or early apoptotic cells) on mus musculus mouse C2C12 cells after 24 h of treatment. Incubation of mus musculus mouse C2C12 cells with [1,2-13C2]-glucose during 24 h, subsequent quantification of 13C isotopologues distribution in key metabolites of glucose metabolic network and a computational fluxomic analysis using in-house developed software (Isodyn) showed that PPD is inhibiting glycolysis, non-oxidative pentose phosphate pathway, glycogen turnover, and ATPAse reaction leading to a reduction in ATP synthesis. These findings unveil the glucose metabolism collapse, which is consistent with a decrease in cell viability observed in PPD-treated C2C12 cells and with the myoglubinuria and other effects observed in Wistar Rats treated with PPD. These findings shed new light on muscle dysfunction associated to PPD exposure, opening new avenues for cost-effective therapies in Rhabdomyolysis disease

Changes in a Comprehensive Profile of Saliva Analytes in Fattening Pigs during a Complete Productive Cycle : A Longitudinal Study

The aim of this study was to evaluate whether a panel of 29 salivary biomarkers of stress, immunity, inflammation, redox homeostasis and other physiological functions can change in healthy fattening pigs when monitoring the different phases of their productive cycle and can be influenced by various sources of variations such as gender and performance parameters. Several analytes showed changes due to the productive cycle, with a majority of the analytes showing higher values at lactation and at the beginning of nursery. Additionally, differences were seen due to sex. These differences can be related in some cases with performance parameters and should be taken into consideration for an appropriate interpretation of the analytes. A comprehensive panel of 29 salivary analytes was measured in fattening pigs to evaluate its possible changes along their productive cycle. The identification of those changes would allow a better interpretation of the results according to the productive phase of the animal. Saliva samples were obtained from 49 Large-White pigs (24 females, 25 males) in suckling phase, at the beginning and the end of the nursery phase, and at the beginning and the end of the growing phase. Several analytes changed according to the phase of the productive cycle, with most of the analytes showing higher values at lactation and at the beginning of nursery. Additionally, differences were seen due to sex. When possible relations between performance parameters and analytes were evaluated, significant positive but weak relationships were found between weight at birth and salivary γ-glutamyl transferase, and between back-fat thickness and salivary lactate dehydrogenase. In conclusion, differences in the values of salivary analytes can be found in fattening pigs depending on the productive phase and sex of the animals