Thiol-based redox regulation in sexual plant reproduction: new insights and perspectives

The success of sexual reproduction in plants involves (i) the proper formation of the plant gametophytes (pollen and embryo sac) containing the gametes, (ii) the accomplishment of specific interactions between pollen grains and the stigma, which subsequently lead to (iii) the fusion of the gametes and eventually to (iv) the seed setting. Owing to the lack of mobility, plants have developed specific regulatory mechanisms to control all developmental events underlying the sexual plant reproduction according to environmental challenges. Over the last decade, redox regulation and signaling have come into sight as crucial mechanisms able to manage critical stages during sexual plant reproduction. This regulation involves a complex redox network which includes reactive oxygen species (ROS), reactive nitrogen species (RNS), glutathione and other classic buffer molecules or antioxidant proteins, and some thiol/disulphide-containing proteins belonging to the thioredoxin superfamily, like glutaredoxins (GRXs) or thioredoxins (TRXs). These proteins participate as critical elements not only in the switch between the mitotic to the meiotic cycle but also at further developmental stages of microsporogenesis. They are also implicated in the regulation of pollen rejection as the result of self-incompatibility. In addition, they display precise space-temporal patterns of expression and are present in specific localizations like the stigmatic papillae or the mature pollen, although their functions and subcellular localizations are not clear yet. In this review we summarize insights and perspectives about the presence of thiol/disulphide-containing proteins in plant reproduction, taking into account the general context of the cell redox network. [EN]European Regional Development Fund (ERDF) through the projects BFU2011-22779, P2010-AGR6274, P2010-CVI5767 and P2011-CVI-7487. Jose A. Traverso thanks Spanish CSIC funding within the frame of JAE-DOC program.Peer reviewe

Application of X-Ray microanalysis, diffraction and cytochemical techniques in the study of the structure and chemical composition of inclusions in Olea europaea leaves

4 páginas, 11 figuras.-- Trabajo presentado al EMAG-MICRO 89 celebrado en Londres (Inglaterra) en Septiembre de 1989.Two types of inclusions have been found in mesophyll cells oÍ leaves of Olea eurooaea. The first type is located in the vacuole, and the application of X-Ray microanalysis, X-Ray diffraction and cytochemical techniques shown that these inclusions are composed of calcium oxalate. The second type of inclusion is intranuclear and its proteic nature is demonstrated by means of light microscopy stains. These crystal structures are probably well ordered in three dimensions.Peer reviewe

The use of in situ hybridisation and immunocytochemistry to characterise coiled bodies in plant meiocytes

2 páginas, 2 figuras.-- Trabajo presentado a la XI European Conference on Electron Microscopy- EUREM11 celebrada en Dublín (Irlanda) del 26 al 30 de Agosto de 1996.This study was supported by project DGICYT PB92-0079-CO3-O3.Peer reviewe

Proposal of the Dichotomous STATIS DUAL Method: Software and Application for the Analysis of Dichotomous Data, Applied to the Test of Learning Styles in University Students

The present work analyzed a review of methods for analyzing sequences of matrices or dichotomous data. A new method for a sequence of dichotomous matrices with a different number of rows is presented; the Dichotomous STATIS DUAL. Suppose we match the sequence of matrices by different years, with this method. In that case, we can graphically represent the relations among the different columns of all the matrices, and the relations between those columns and the different years, because everything can be represented in the same plots. As in all STATIS methods, three different plots can get (i) the interstructure, with the relations among the years; (ii) the compromise, with the stable part of the relations between the columns; and (iii) the intrastructure (also known as trajectories), with the relations between columns and years, in other words, the evolution of the columns through the time. This new mathematical method can be used with all kinds of dichotomous data, thanks to the software we propose. In the present work, the software was applied to the assessment of learning styles

Expression profiling and localization of wall polysaccharides in the olive pollen during in vitro germination

1 página.-- Comunicación oral presentada en la XVIII Reunión de la Sociedad Española de Fisiología Vegetal (SEFV).-- Sesión Temática 2: Crecimiento y desarrollo.-- S2-O2.This work was supported by the Andalusian Regional Government (project P06-AGR-01791). C. Suarez thanks the MEC for providing FPI grant funding.Peer reviewe

Innovación abierta en tecnología, diseño y dinámicas sociales

ITESO, A.C

Genetic diversity of meat quality related genes in Argentinean pigs

Genetic influence on pork quality exists between breeds and within a breed. The variation is caused by a large set of genes, and pork quality traits have a multifactorial background. Research into the genetics of meat quality found causative mutations associated with marked effects on pig meat value. This study aimed to investigate the segregation of meat quality-related SNPs and compare their diversity and genetics in commercial and Creole pigs from different farms in the North-West of Argentina. A screen for SNPs in RYR1, PRKAG3, CAST, and SOX6 candidate genes and the differentiation of their genotypes by PCR–RFLP was conducted. All genes were characterized by a high level of polymorphism and heterozygosity, and populations showed no differences in the genetic structure for the analyzed SNPs. These results highlighted the role of pig genotypes as a source of basic variability potentially affecting processed meat products and fresh meat.Fil: Rodríguez, Viviana Rita. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; ArgentinaFil: Maffioly, J. I.. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; ArgentinaFil: Zdanovicz, L. A.. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; ArgentinaFil: Fabre, Romina María. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; ArgentinaFil: Barrandeguy, Maria Eugenia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: García, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Genética; ArgentinaFil: Lagadari, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentin

Proteínas de almacenamiento tipo 11S en semillas de olivo (Olea europaea L.). Caracterización mediante técnicas de proteómica

6 páginas, 3 figuras, 1 tabla.-- Trabajo presentado al Simposium Científico–Técnico EXPOLIVA 2007.Los tejidos de reserva de la semilla del olivo acumulan grandes cantidades de proteínas de almacenamiento en forma de cuerpos proteicos. En este trabajo se analizaron extractos proteicos de cotiledón y endospermo mediante electroforesis bidimensional (2-D). Dichas técnicas permitieron detectar el carácter básico de los péptidos p1 y p2, así como el carácter ácido de p3, p4 y p5. La espectrometría de masas de diversos spot aislados, indicó una homología relevante con la subunidad básica de las leguminas de tipo 11S. También se detectó la presencia de diversas isoformas, algunas posiblemente resultantes de modificaciones postranscripcionales. La abundancia de estas proteínas en las semillas maduras de olivo hace que dicho material pueda ser considerado una fuente nitrogenada de posible uso como complemento en nutrición animal. Otras utilidades relevantes podrían explotar un posible carácter discriminatorio entre variedades para el desarrollo de programas de mejora genética en olivo actualmente en curso.Este trabajo fue financiado gracias a los proyectos AGL2003-00719 y MEC BFU2004- 00601/BFI. Los autores agradecen al Consejo Superior de Investigaciones Científicas (CSIC) la concesión de una beca predoctoral (I3P-CSIC 2002).Peer reviewe

Estimation of time dedication to a pathophysiology practice

Resúmenes IV Congreso VetDoc de Docencia Veterinaria, León 2017 (6-7 de Julio)[ES] El objetivo de este trabajo fue comprobar si la carga lectiva real de la práctica “estudio de caso” correspondiente a la parte de fisiopatología se correlacionaba adecuadamente con la establecida en el plan docente