Copas cerámicas para vino como material ancestral alternativo –revalorizando el patrimonio cultural vitivinícola

Comunicación científica oral de equipos de investigación realizada en el IV Encuentro de Investigadores de la Red Andina de Universidades (RADU) y las XIV Jornadas de Investigación UMaza 2022. Bloque de comunicaciones científicas del tema: CIENCIAS ENOLÓGICAS, el día 24 de agosto del 2022, moderado por Martín Fanzone y Jorge Prieto. Las jornadas se llevaron adelante desde 23 al 24 de agosto del 2022 en formato totalmente virtual bajo plataforma Zoom y fueron transmitidas por el canal YouTube de la UMaza y el Facebook del Área de Ciencia y Técnica UMaza (Somos Ciencia y Técnica UMaza)

Representative sequencing: Unbiased sampling of solid tumor tissue

International audienceAlthough thousands of solid tumors have been sequenced to date, a fundamental under-sampling bias isinherent in current methodologies. This is caused by a tissue sample input of fixed dimensions (e.g., 6 mmbiopsy), which becomes grossly under-powered as tumor volume scales. Here, we demonstrate representative sequencing (Rep-Seq) as a new method to achieve unbiased tumor tissue sampling. Rep-Seq uses fixed residual tumor material, which is homogenized and subjected to next-generation sequencing. Analysis of intratumor tumor mutation burden (TMB) variability shows a high level of misclassification using current single-biopsy methods, with 20% of lung and 52% of bladder tumors having at least one biopsy with high TMB butlow clonal TMB overall. Misclassification rates by contrast are reduced to 2% (lung) and 4% (bladder) when a more representative sampling methodology is used. Rep-Seq offers an improved sampling protocol for tumor profiling, with significant potential for improved clinical utility and more accurate deconvolution of clonal structure