8 research outputs found
Analysis and mapping of impacts under climate change for adaptation and food security (AMICAF) project in Paraguay
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User experience and motivation with engineering design challenges in general chemistry laboratory; A Valve-Enabled Sample Preparation Device with Isothermal Amplification for Multiplexed Virus Detection at the Point-of-Care
Early and accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza viruses at the point-of-care is crucial for reducing disease transmission during the current pandemic and future flu seasons. To prepare for potential cocirculation of these two viruses, we report a valve-enabled, paper-based sample preparation device integrated with isothermal amplification for their simultaneous detection. The device incorporates (1) virus lysis and RNA enrichment, enabled by ball-based valves for sequential delivery of reagents with no pipet requirement, (2) reverse transcription loop-mediated isothermal amplification, carried out in a coffee mug, and (3) colorimetric detection. We have used the device for simultaneously detecting inactivated SARS-CoV-2 and influenza A H1N1 viruses in 50 min, with limits of detection at 2 and 6 genome equivalents, respectively. The device was further demonstrated to detect both viruses in environmental samples
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Valve-Enabled Sample Preparation and Isothermal Amplification for SARS-CoV-2 Detection at the Point-of-Care
Abstract Early and accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) at the point-of-care (POC) is crucial for reducing the transmission of coronavirus disease 2019 (COVID-19). To address this need, we have developed a valve-enabled lysis, paper-based RNA enrichment, and RNA amplification device (VLEAD) for detecting SARS-CoV-2. We have combined VLEAD with a smart coffee mug for sample preparation, nucleic acid isothermal amplification, and colorimetric detection using a smartphone camera or a naked eye. VLEAD enables two critical functions required for POC testing: sample preparation and detection. Since the reagents can be pre-packaged in the device, all operational steps can be carried out at POC. We have demonstrated the functions of the device by analyzing samples spiked with heat-inactivated SARS-CoV-2, which were obtained from BEI Resources. This rapid and highly sensitive POC platform for SARS-CoV-2 detection has a potential to help reduce COVID-19 transmission
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Detection and isolation of infectious SARS-CoV-2 omicron subvariants collected from residential settings
Puesta en práctica de un programa de prácticas de Oftalmología en la Licenciatura de Medicina en el nuevo plan de estudios
Material no publicadoProyecto desarrollado en el Instituto de Oftalmobiología Aplicada. Los profesores implicados pertenecen a este instituto y ejercen docencia en Medicina y Óptica-Optometría. Objetivos: mejorar la cualificación docente (cursos de formación pedagógica y autoevaluación), mejorar la calidad de la enseñanza y de la práctica mediante la elaboración de objetivos de aprendizaje basados en competencias y un cuaderno de prácticas para los alumnos, adquirir nuevo material de prácticas, desarrollar ayudas audiovisuales, iniciar el programa de calidad en la docencia práctica mediante evaluación y actividades de mejora pactadas. La metodología con la que este proyecto de ha desarrollado ha sido: reuniones de grupo para el diseño de objetivos, cursos de formación pedagógica y encuestas de evaluación. El proceso se ha aplicado en dos cursos de la licenciatura en medicina. Los materiales han sido solicitados por otras universidades. Las encuestas demuestran mejoras en los aprendizajes.Junta de Castilla y León. Dirección General de Universidades e InvestigaciónCastilla y LeónES