Development and characterization of an eggplant (Solanum melongena) doubled haploid population and a doubled haploid line with high androgenic response

[EN] We developed an eggplant doubled haploid (DH) population from a commercial hybrid through androgenesis in microspore culture. Morphological variation, reproductive ability and androgenic responsiveness were evaluated. The DH population showed segregation in vegetative traits related to leaf, flower and fruit, and in reproductive traits such as fruit and seed setting or germination rate. The DH population and subsequent generations also presented variation in the androgenic response, with null, low and high response lines. From this population, we were able to identify the first eggplant highly androgenic DH line (DH36), remarkably similar to the donor hybrid in terms of morphology and reproductive ability, but stably producing four times more calli than the hybrid. The segregating DH population is potentially useful for genetic studies and mapping of several traits, whereas the highly androgenic line DH36 may be used as a model line to facilitate the study of eggplant androgenesis and embryogenesis for both basic and applied research.We would like to thank the reviewers of this manuscript for their critical and helpful comments. This work was supported by Grant AGL2014-55177-R to JMSS from Spanish Ministerio de Economia y Competitividad (MINECO) jointly funded by FEDER. ARS is supported by a Predoctoral Fellowship from the FPI Program of Universitat Politecnica de Valencia.Rivas-Sendra, A.; Manuel Campos-Vega; Calabuig-Serna, A.; Seguí-Simarro, JM. (2017). Development and characterization of an eggplant (Solanum melongena) doubled haploid population and a doubled haploid line with high androgenic response. 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Liderazgo directivo y desempeño docente en las instituciones educativas de la RED de la No. 16 del distrito de Villa El Salvador, 2013

El objetivo de la investigación fue determinar la relación existente entre liderazgo directivo y desempeño docente en las instituciones educativas de la Red Nº 16 Villa El Salvador, 2013. Como estos se han aplicado en determinar la relación que existe entre el Liderazgo directivo y Desempeño docente. La investigación busca determinar los factores que se asocian en un Liderazgo directivo y plantear estrategias para una gestión eficaz en las Instituciones Educativas. El estudio realizado es de tipo básico, su diseño es descriptivo correlacional. La población de estudio en la investigación está conformada por 457 participantes de ocho Instituciones Educativas de la Red 16 de Villa El Salvador, de la Ugel 01 y una muestra de 211 participantes. Como instrumento de diagnóstico fue utilizado el cuestionario para establecer la relación entre las variables. La investigación se ha elaborado bajo los procedimientos metodológicos del enfoque cuantitativo, estructura del diseño de investigación no experimental, del tipo correlacional. Los datos obtenidos del instrumento aplicado fueron procesados mediante un software estadístico denominado SPSS versión 21. En los casos de las variables de Liderazgo directivo y Desempeño docente, se ha construido un instrumento que ha sido validado mediante el juicio de expertos. En cuanto a los resultados podemos mencionar que se estableció una correlación significativa entre variable 1 de Liderazgo directivo y la variable 2 de Desempeño docente. Se concluye que si existe relación entre las variables propuestas

Efecto antilipoperoxidante de Plukenetia volubilis L. (Sacha inchi) en ratas con diabetes inducida por aloxano.

Con el objetivo de  demostrar el efecto  antilipoperoxidante de   Plukenetia volubilis L. (Sacha Inchi). en ratas con diabetes inducida por aloxano. Método: se diseñó  un estudio experimental de casos y controles. 24 ratas con diabetes inducida por aloxano, fueron distribuida al azar en   4 grupos: (G0) Grupo control que recibieron 0,64 ml/kg de etanol,  (G1) Grupo estándar tratado con  de 0,25 ml/kg de vitamina E,  (G2) Grupo Experimental tratado con 120 mg/kg de extracto fluido de Sacha Inchi, (G3) Grupo Experimental tratado con  140 mg/kg de extracto fluido de Sacha Inchi.En todos los especímenes se determinó   nivel sérico de Malon dialdehido (MDA) uno basal y otro a los  16 días de iniciado el experimento.  Resultados: G1 mostró una reducción significativa de los niveles de Malondialdehido (MDA) sérico de 30% con respecto al control. El grupo G2, aproximadamente del 16.18% con respecto al control y el grupo G3 también mostró una reducción significativa del 23.5% con respecto al grupo control. Conclusión: la administración del extracto de hojas de Plukenetia Volubilis L in vivo tiene efecto antilipoperoxidante en ratas.  El mismo que es dependiente de  la dosis  administrada. Palabras clave: lipoperoxidación, glicemia, Plukenetia volubilis L., diabetes, estrés oxidativo

Filosofía, Ciencia y Cine: interpretaciones filosóficas de las ciencias y sus repercusiones sociales en la ciencia ficción

Este proyecto de innovación docente se centra en el uso filosófico de herramientas audiovisuales en el género de la ciencia ficción con el objetivo de promover la reflexión crítica de conceptos y contenidos particularmente abstractos y transdisciplinares

Glucose Transporter 1 and Monocarboxylate Transporters 1, 2, and 4 Localization within the Glial Cells of Shark Blood-Brain-Barriers

Although previous studies showed that glucose is used to support the metabolic activity of the cartilaginous fish brain, the distribution and expression levels of glucose transporter (GLUT) isoforms remained undetermined. Optic/ultrastructural immunohistochemistry approaches were used to determine the expression of GLUT1 in the glial blood-brain barrier (gBBB). GLUT1 was observed solely in glial cells; it was primarily located in end-feet processes of the gBBB. Western blot analysis showed a protein with a molecular mass of 50 kDa, and partial sequencing confirmed GLUT1 identity. Similar approaches were used to demonstrate increased GLUT1 polarization to both apical and basolateral membranes in choroid plexus epithelial cells. To explore monocarboxylate transporter (MCT) involvement in shark brain metabolism, the expression of MCTs was analyzed. MCT1, 2 and 4 were expressed in endothelial cells; however, only MCT1 and MCT4 were present in glial cells. In neurons, MCT2 was localized at the cell membrane whereas MCT1 was detected within mitochondria. Previous studies demonstrated that hypoxia modified GLUT and MCT expression in mammalian brain cells, which was mediated by the transcription factor, hypoxia inducible factor-1. Similarly, we observed that hypoxia modified MCT1 cellular distribution and MCT4 expression in shark telencephalic area and brain stem, confirming the role of these transporters in hypoxia adaptation. Finally, using three-dimensional ultrastructural microscopy, the interaction between glial end-feet and leaky blood vessels of shark brain was assessed in the present study. These data suggested that the brains of shark may take up glucose from blood using a different mechanism than that used by mammalian brains, which may induce astrocyte-neuron lactate shuttling and metabolic coupling as observed in mammalian brain. Our data suggested that the structural conditions and expression patterns of GLUT1, MCT1, MCT2 and MCT4 in shark brain may establish the molecular foundation of metabolic coupling between glia and neurons

5to. Congreso Internacional de Ciencia, Tecnología e Innovación para la Sociedad. Memoria académica

El V Congreso Internacional de Ciencia, Tecnología e Innovación para la Sociedad, CITIS 2019, realizado del 6 al 8 de febrero de 2019 y organizado por la Universidad Politécnica Salesiana, ofreció a la comunidad académica nacional e internacional una plataforma de comunicación unificada, dirigida a cubrir los problemas teóricos y prácticos de mayor impacto en la sociedad moderna desde la ingeniería. En esta edición, dedicada a los 25 años de vida de la UPS, los ejes temáticos estuvieron relacionados con la aplicación de la ciencia, el desarrollo tecnológico y la innovación en cinco pilares fundamentales de nuestra sociedad: la industria, la movilidad, la sostenibilidad ambiental, la información y las telecomunicaciones. El comité científico estuvo conformado formado por 48 investigadores procedentes de diez países: España, Reino Unido, Italia, Bélgica, México, Venezuela, Colombia, Brasil, Estados Unidos y Ecuador. Fueron recibidas un centenar de contribuciones, de las cuales 39 fueron aprobadas en forma de ponencias y 15 en formato poster. Estas contribuciones fueron presentadas de forma oral ante toda la comunidad académica que se dio cita en el Congreso, quienes desde el aula magna, el auditorio y la sala de usos múltiples de la Universidad Politécnica Salesiana, cumplieron respetuosamente la responsabilidad de representar a toda la sociedad en la revisión, aceptación y validación del conocimiento nuevo que fue presentado en cada exposición por los investigadores. Paralelo a las sesiones técnicas, el Congreso contó con espacios de presentación de posters científicos y cinco workshops en temáticas de vanguardia que cautivaron la atención de nuestros docentes y estudiantes. También en el marco del evento se impartieron un total de ocho conferencias magistrales en temas tan actuales como la gestión del conocimiento en la universidad-ecosistema, los retos y oportunidades de la industria 4.0, los avances de la investigación básica y aplicada en mecatrónica para el estudio de robots de nueva generación, la optimización en ingeniería con técnicas multi-objetivo, el desarrollo de las redes avanzadas en Latinoamérica y los mundos, la contaminación del aire debido al tránsito vehicular, el radón y los riesgos que representa este gas radiactivo para la salud humana, entre otros

Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality

Goodbye Hartmann trial: a prospective, international, multicenter, observational study on the current use of a surgical procedure developed a century ago

Background: Literature suggests colonic resection and primary anastomosis (RPA) instead of Hartmann's procedure (HP) for the treatment of left-sided colonic emergencies. We aim to evaluate the surgical options globally used to treat patients with acute left-sided colonic emergencies and the factors that leading to the choice of treatment, comparing HP and RPA. Methods: This is a prospective, international, multicenter, observational study registered on ClinicalTrials.gov. A total 1215 patients with left-sided colonic emergencies who required surgery were included from 204 centers during the period of March 1, 2020, to May 31, 2020. with a 1-year follow-up. Results: 564 patients (43.1%) were females. The mean age was 65.9 ± 15.6 years. HP was performed in 697 (57.3%) patients and RPA in 384 (31.6%) cases. Complicated acute diverticulitis was the most common cause of left-sided colonic emergencies (40.2%), followed by colorectal malignancy (36.6%). Severe complications (Clavien-Dindo ≥ 3b) were higher in the HP group (P < 0.001). 30-day mortality was higher in HP patients (13.7%), especially in case of bowel perforation and diffused peritonitis. 1-year follow-up showed no differences on ostomy reversal rate between HP and RPA. (P = 0.127). A backward likelihood logistic regression model showed that RPA was preferred in younger patients, having low ASA score (≤ 3), in case of large bowel obstruction, absence of colonic ischemia, longer time from admission to surgery, operating early at the day working hours, by a surgeon who performed more than 50 colorectal resections. Conclusions: After 100 years since the first Hartmann's procedure, HP remains the most common treatment for left-sided colorectal emergencies. Treatment's choice depends on patient characteristics, the time of surgery and the experience of the surgeon. RPA should be considered as the gold standard for surgery, with HP being an exception