Coagulase gene polymorphism of Staphylococcus aureus isolated from clinical and sub-clinical bovine mastitis in Isfahan and Chaharmahal va Bakhtiari provinces of Iran

Mastitis is a common disease in dairy cattle and is an inflammatory response of the breast tissue to bacterial attack to this tissue. Mastitis causes considerable loss to the dairy industry, among the several bacterial pathogens that can cause mastitis; Staphylococcus aureus is probably the most lethal agent because it causes chronic and deep infection in the mammary glands that is extremely difficult to cure. Several virulence factors including coagulase gene are produced by S. aureus and may contribute to its pathogenicity. This study was conducted to investigate the coagulase gene polymorphism of S. aureus isolated from clinical and sub-clinical bovine mastitis milk samples in Isfahan and Chaharmahal va Bakhtiari provinces of Iran. Amplification of the coagulase gene from 86 S. aureus strains isolates by specific primers showed 31 specimens contained 970 bp fragment, and 11 strains contained 730 bp fragment relevant to coa gene (coagulase) in PCR. After enzymatic digestion with AluI, 31 specimens contained three bands: 320, 490, and 160 bp (genotype I) and 11 specimens contained two bands: 490 and 240 bp (genotype VIII) in the RFLP

Evaluate the frequency of respiratory syncytial virus (RSV) in dairy herds in the Chaharmahal va Bakhtyari province - IRAN

Background and Aim: Bovine respiratory syncytial viruses (BRSV) are important causes of acute respiratory disease in postweaning calves and feedlot cattle in the United States and Europe. This disease poses a serious problem due to financial losses caused by animal death, costs of treatment, and subsequent reduced profits. So the aim of this study was evaluate the frequency of respiratory syncytial virus (RSV) in dairy herds in the Chaharmahal va Bakhtyari province - IRAN Materials and Methods: In this research, 384 serum samples of cattle were collected from Chaharmahal va Bakhtiari provience - Iran and tested by nested RT-PCR. For detection of BRSV genome, at the first viral RNA was extracted by RNX Plus kit (Cinnagen Company) then cDNA was synthesize. Positive control used in this study was the positive control in BioinGentech (Vet PCRTM Detection Kit).  Results: A total of 384 serum samples in Chaharmahal va Bakhtiari provience 300 samples were positive in nested RT-PCR. Prevalence rate were determined 78.12%. The highest contamination were reported in Shahrekord city, females and in Winter. Conclusions: BRSV infection has been reported in all parts of the world. Regardless of geographic location, the prevalence of BIV infection is high. This indicates that the virus is easily transmitted to cattle. These findings are important for effective control management to prevent the spread of the virus that is associated with various methods of agriculture. Therefore, more information to understand the mechanisms of virus survival in a geographic area is required