1,123 research outputs found
Greenhouse gas measurement capability at the Carbon Related Atmospheric Measurement (CRAM) Laboratory at the University of East Anglia, United Kingdom
Contribution of bacterial outer membrane vesicles to innate bacterial defense
<p>Abstract</p> <p>Background</p> <p>Outer membrane vesicles (OMVs) are constitutively produced by Gram-negative bacteria throughout growth and have proposed roles in virulence, inflammation, and the response to envelope stress. Here we investigate outer membrane vesiculation as a bacterial mechanism for immediate short-term protection against outer membrane acting stressors. Antimicrobial peptides as well as bacteriophage were used to examine the effectiveness of OMV protection.</p> <p>Results</p> <p>We found that a hyper-vesiculating mutant of <it>Escherichia coli </it>survived treatment by antimicrobial peptides (AMPs) polymyxin B and colistin better than the wild-type. Supplementation of <it>E. coli </it>cultures with purified outer membrane vesicles provided substantial protection against AMPs, and AMPs significantly induced vesiculation. Vesicle-mediated protection and induction of vesiculation were also observed for a human pathogen, enterotoxigenic <it>E. coli </it>(ETEC), challenged with polymyxin B. When ETEC with was incubated with low concentrations of vesicles concomitant with polymyxin B treatment, bacterial survival increased immediately, and the culture gained resistance to polymyxin B. By contrast, high levels of vesicles also provided immediate protection but prevented acquisition of resistance. Co-incubation of T4 bacteriophage and OMVs showed fast, irreversible binding. The efficiency of T4 infection was significantly reduced by the formation of complexes with the OMVs.</p> <p>Conclusions</p> <p>These data reveal a role for OMVs in contributing to innate bacterial defense by adsorption of antimicrobial peptides and bacteriophage. Given the increase in vesiculation in response to the antimicrobial peptides, and loss in efficiency of infection with the T4-OMV complex, we conclude that OMV production may be an important factor in neutralizing environmental agents that target the outer membrane of Gram-negative bacteria.</p
Spatio-temporal bivariate statistical models for atmospheric trace-gas inversion
Atmospheric trace-gas inversion refers to any technique used to predict
spatial and temporal fluxes using mole-fraction measurements and atmospheric
simulations obtained from computer models. Studies to date are most often of a
data-assimilation flavour, which implicitly consider univariate statistical
models with the flux as the variate of interest. This univariate approach
typically assumes that the flux field is either a spatially correlated Gaussian
process or a spatially uncorrelated non-Gaussian process with prior expectation
fixed using flux inventories (e.g., NAEI or EDGAR in Europe). Here, we extend
this approach in three ways. First, we develop a bivariate model for the
mole-fraction field and the flux field. The bivariate approach allows optimal
prediction of both the flux field and the mole-fraction field, and it leads to
significant computational savings over the univariate approach. Second, we
employ a lognormal spatial process for the flux field that captures both the
lognormal characteristics of the flux field (when appropriate) and its spatial
dependence. Third, we propose a new, geostatistical approach to incorporate the
flux inventories in our updates, such that the posterior spatial distribution
of the flux field is predominantly data-driven. The approach is illustrated on
a case study of methane (CH) emissions in the United Kingdom and Ireland.Comment: 39 pages, 8 figure
Self-starting optical–electrical–optical homodyne clock recovery for phase-modulated signals
We propose a novel self-homodyne optical–electrical–optical clock recovery technique for binary phase-shift keying (BPSK) signals using commercial optical and electrical components. We present the principle of operation as well as a proof-of-concept experiment for a 10.7 Gb/s BPSK signal clock recovery transmitted over a dispersion-compensated link of 20 km of single-mode fiber. Suppression of pattern-related frequency noise at the output of the recovered clock is shown. The timing jitter of the recovered clock at 10.7 GHz was measured to be ∼450 fs (integration range: 100 Hz–10 MHz)
A Serendipitous Search for High-Redshift Lyman alpha Emission: Two Primeval Galaxy Candidates at z~3
In the course of our ongoing search for serendipitous high-redshift Lyman
alpha (LyA) Emissionin deep archival Keck spectra, we discovered two very high
equivalent width (W_{obs} ~ 450A, 2-sigma) LyA emission line candidates at z ~3
in a moderate dispersion (R~1200) spectrogram. Both lines have low velocity
dispersions (sigma_v ~ 60 km/s) and deconvolved radii r ~ 1 kpc (h = 0.5). We
argue that the lines are LyA, and are powered by stellar ionization. The
surface density of robust, high equivalent width LyA candidates is estimated to
be ~3 \pm 2 per arcmin^2 per unit redshift at z ~ 3, consistent with the
estimate of Cowie etal (1998). The LyA emission line source characteristics are
consistent with the galaxies undergoing their first burst of star formation,
ie, with being primeval. Source sizes and velocity dispersions are comparable
to the theoretical primeval galaxy model of Lin and Murray (1992) based on the
inside-out, self-similar collapse of an isothermal sphere. In this model, star
formation among field galaxies is a protracted process. Galaxies are thought to
be able to display high equivalent widths for only the first few x 10 Myr. This
time is short in relation to the difference in look back times between z=3 and
z=4, and implies that a substantial fraction of strong line-emitting galaxies
at z=3 were formed at redshifts z < 4. We discuss the significance of
high-equivalent width LyA-emitting galaxies in terms of the emerging picture of
the environment, and the specific characteristics of primeval galaxy formation
at high redshift.Comment: 17 pages, 3 figures, one table. To appear in the Astrophysical
Journa
Morphodynamics in a Tropical Shallow Lagoon: Observation and Inferences of Change
The Lagos Lagoon system and its adjacent tidal basins exhibit dynamics that are significantly different on both spatial and temporal scales. As urbanisation and human activities around the lagoon have intensified, the volume of sediment deposited into the basin is increasing on a daily basis. Changes on the lagoon bed over a 6-year time scale using repeated bathymetric data (2008, 2014) are presented, and the related data acquisition technique is explained. Data reduction is followed by analysis of the lagoon water bed dynamics using abstracted profile lines from the bathymetric data within a GIS environment. The results of the significant accretion and erosion within the lagoon system were analyzed spatially to quantify the volume of sediment gain or loss on the lagoon bed. The findings partly show that over 6 years, an average height of 0.16 m was gained by the lagoon. This amount translates into an annual accretion rate of 0.026 m. These findings enhance the prospect of verifying in the long term whether the Lagos Lagoon is gradually disappearing. To the best of the author’s knowledge, this research reveals for the first time the complex evolutionary changes (channel movement, accretion, erosion, infill and movement of shoal) on the Lagos Lagoon bed
Interpreting the seasonal cycles of atmospheric oxygen and carbon dioxide concentrations at American Samoa Observatory
We present seven years of atmospheric O2/N2 ratio and CO2 concentration data measured from flask samples collected at American Samoa. These data are unusual, exhibiting higher short-term variability, and seasonal cycles not in phase with other sampling stations. The unique nature of atmospheric data from Samoa has been noted previously from measurements of CO2, methyl chloroform, and ozone. With our O2 data, we observe greater magnitude in the short-term variability, but, in contrast, no clear seasonal pattern to this variability. This we attribute to significant regional sources and sinks existing for O2 in both hemispheres, and a dependence on both the latitudinal and altitudinal origins of air masses. We also hypothesize that some samples exhibit a component of "older" air, demonstrating recirculation of air within the tropics. Our findings could be used to help constrain atmospheric transport models which are not well characterized in tropical regions
Discovery of a Substrate Selectivity Switch in Tyrosine Ammonia-Lyase, a Member of the Aromatic Amino Acid Lyase Family
SummaryTyrosine ammonia-lyase (TAL) is a recently described member of the aromatic amino acid lyase family, which also includes phenylalanine (PAL) and histidine ammonia-lyases (HAL). TAL is highly selective for L-tyrosine, and synthesizes 4-coumaric acid as a protein cofactor or antibiotic precursor in microorganisms. In this report, we identify a single active site residue important for substrate selection in this enzyme family. Replacing the active site residue His89 with Phe in TAL completely switched its substrate selectivity from tyrosine to phenylalanine, thereby converting it into a highly active PAL. When a corresponding mutation was made in PAL, the enzyme lost PAL activity and gained TAL activity. The discovered substrate selectivity switch is a rare example of a complete alteration of substrate specificity by a single point mutation. We also show that the identity of the amino acid at the switch position can serve as a guide to predict substrate specificities of annotated aromatic amino acid lyases in genome sequences
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