Climate Change Impacts in Virginia: Status of Natural Resource Data Records as Tools to Assess Continuing Trends

As scientists we pose the hypothesis that climate change over the past decades has left a signal in natural resource status and productivity in Virginia. This signal exists subsumed in a variety of data from crop and forestry production, to fishery landings, to spatial distribution of numerous plant and animal species of interest. Description of these signals in concert with known changes in climate descriptors (temperature, rainfall and more) provide a basis for hind-casting possible cause and effect relationships. If such relationships exist, and we hypothesize that they do, then projections of climate descriptors (temperature, rainfall and more) provide the basis for projections of impacts on defined natural resources, with obvious economic and societal impacts. Before a comprehensive analysis of extant data can occur we must start with a simple inventory of available data. As simplistic as this may sound we can find no single database that describes the general status of natural resources in Virginia over the past decades. Indeed, we suggest that the majority of such data exists as unpublished (in peer review, and in some instances even technical reports) compilations spread among the various state and federal natural resource agencies active in Virginia. This body of work had several goals; 1) to assess the scope of natural resource descriptive data available in the Commonwealth of Virginia, 2) to assemble an index of such data, and 3) develop a bibliography to serve as a resource for more comprehensive analyses in the future

An explicit SU(12) family and flavor unification model with natural fermion masses and mixings

We present an SU(12) unification model with three light chiral families, avoiding any external flavor symmetries. The hierarchy of quark and lepton masses and mixings is explained by higher dimensional Yukawa interactions involving Higgs bosons that contain SU(5) singlet fields with VEVs about 50 times smaller than the SU(12) unification scale. The presented model has been analyzed in detail and found to be in very good agreement with the observed quark and lepton masses and mixings.Comment: 11 pages, 4 table

SOAR TESS Survey. II: The impact of stellar companions on planetary populations

We present the results of the second year of exoplanet candidate host speckle observations from the SOAR TESS survey. We find 89 of the 589 newly observed TESS planet candidate hosts have companions within 3\arcsec, resulting in light curve dilution, that if not accounted for leads to underestimated planetary radii. We combined these observations with those from paper I to search for evidence of the impact binary stars have on planetary systems. Removing the quarter of the targets observed identified as false-positive planet detections, we find that transiting planet are suppressed by nearly a factor-of-seven in close solar-type binaries, nearly twice the suppression previously reported. The result on planet occurrence rates that are based on magnitude limited surveys is an overestimation by a factor of two if binary suppression is not taken into account. We also find tentative evidence for similar close binary suppression of planets in M-dwarf systems. Lastly, we find that the high rates of widely separated companions to hot Jupiter hosts previously reported was likely a result of false-positive contamination in our sample.Comment: Accepted to A

Imaging Gold Nanoparticles in Living Cells Environments using Heterodyne Digital Holographic Microscopy

This paper describes an imaging microscopic technique based on heterodyne digital holography where subwavelength-sized gold colloids can be imaged in cell environment. Surface cellular receptors of 3T3 mouse fibroblasts are labeled with 40 nm gold nanoparticles, and the biological specimen is imaged in a total internal reflection configuration with holographic microscopy. Due to a higher scattering efficiency of the gold nanoparticles versus that of cellular structures, accurate localization of a gold marker is obtained within a 3D mapping of the entire sample's scattered field, with a lateral precision of 5 nm and 100 nm in the x,y and in the z directions respectively, demonstrating the ability of holographic microscopy to locate nanoparticles in living cells environments

Human skin aging is associated with increased expression of the histone variant H2A.J in the epidermis

Cellular senescence is an irreversible growth arrest that occurs as a result of damaging stimuli, including DNA damage and/or telomere shortening. Here, we investigate histone variant H2A.J as a new biomarker to detect senescent cells during human skin aging. Skin biopsies from healthy volunteers of different ages (18–90 years) were analyzed for H2A.J expression and other parameters involved in triggering and/or maintaining cellular senescence. In the epidermis, the proportions of H2A.J-expressing keratinocytes increased from ≈20% in young to ≈60% in aged skin. Inverse correlations between Ki67- and H2A.J staining in germinative layers may reflect that H2A.J-expressing cells having lost their capacity to divide. As cellular senescence is triggered by DNA-damage signals, persistent 53BP1-foci, telomere lengths, and telomere-associated damage foci were analyzed in epidermal keratinocytes. Only slight age-related telomere attrition and few persistent nuclear 53BP1-foci, occasionally colocalizing with telomeres, suggest that unprotected telomeres are not a significant cause of senescence during skin aging. Quantification of integrin-α6+ basal cells suggests that the number and function of stem/progenitor cells decreased during aging and their altered proliferation capacities resulted in diminished tissue renewal with epidermal thinning. Collectively, our findings suggest that H2A.J is a sensitive marker of epidermal aging in human skin

Curriculum development methodologies for English for occupational purposes in tourism management : a case study from a South African university of technology

The development of English for Occupational Purposes (EOP) curricula for universities of technology has become increasingly important. These institutions aim to produce graduates who can function effectively, upon entry into the occupational world. Since English is regarded as the global lingua franca, the demand for EOP is growing worldwide, so that, universally, employees can function in their fields of specialisation. Therefore, EOP wants and needs analyses have become imperative. This implies that the methodologies used to gather data from stakeholders in the development of EOP curricula should be effective. This paper takes a closer look at such methodologies, by exploring the perceptions of stakeholders on some of them, and the value that stakeholders could add to EOP curriculum development for Tourism Management at a South African university of technology.http://www.ajhtl.comScience, Mathematics and Technology Educatio

Role of Histone Variant H2A.J in Fine-Tuning Chromatin Organization for the Establishment of Ionizing Radiation-Induced Senescence

Purpose: Radiation-induced senescence is characterized by profound changes in chromatin organization with the formation of Senescence-Associated-Heterochromatin-Foci (SAHF) and DNASegments-with-Chromatin-Alterations-Reinforcing-Senescence (DNA-SCARS). Importantly, senescent cells also secrete complex combinations of pro-inflammatory factors, referred as Senescence-AssociatedSecretory-Phenotype (SASP). Here, we analyzed the epigenetic mechanism of histone variant H2A.J in establishing radiation-induced senescence. Experimental Design: Primary and genetically-modified lung fibroblasts with down- or up-regulated H2A.J expression were exposed to ionizing radiation and were analyzed for the formation of SAHF and DNA-SCARS by immunofluorescence microscopy. Dynamic changes in chromatin organization and accessibility, transcription factor recruitment, and transcriptome signatures were mapped by ATAC-seq and RNA-seq analysis. The secretion of SASP factors and potential bystander effects were analyzed by ELISA and RT-PCR. Lung tissue of mice exposed to different doses were analyzed by the digital image analysis of H2A.J-immunohistochemistry. Results: Differential incorporation of H2A.J has profound effects on higher-order chromatin organization and on establishing the epigenetic state of senescence. Integrative analyses of ATAC-seq and RNA-seq datasets indicate that H2A.J-associated changes in chromatin accessibility of regulatory regions decisively modulates transcription factor recruitment and inflammatory gene expression, resulting in an altered SASP secretome. In lung parenchyma, pneumocytes show dose-dependent H2A.J expression in response to radiation-induced DNA damage, therefore contributing to proinflammatory tissue reactions. Conclusions: The fine-tuned incorporation of H2A.J defines the epigenetic landscape for driving the senescence programme in response to radiation-induced DNA damage. Deregulated H2A.J deposition affects chromatin remodeling, transcription factor recruitment, and the pro-inflammatory secretome. Our findings provide new mechanistic insights into DNA-damage triggered epigenetic mechanisms governing the biological processes of radiationinduced injury

Identifying the cellular targets of drug action in the central nervous system following corticosteroid therapy

This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Chemical Neuroscience, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/cn400167nCorticosteroid (CS) therapy is used widely in the treatment of a range of pathologies, but can delay production of myelin, the insulating sheath around central nervous system nerve fibers. The cellular targets of CS action are not fully understood, that is, "direct" action on cells involved in myelin genesis [oligodendrocytes and their progenitors the oligodendrocyte precursor cells (OPCs)] versus "indirect" action on other neural cells. We evaluated the effects of the widely used CS dexamethasone (DEX) on purified OPCs and oligodendrocytes, employing complementary histological and transcriptional analyses. Histological assessments showed no DEX effects on OPC proliferation or oligodendrocyte genesis/maturation (key processes underpinning myelin genesis). Immunostaining and RT-PCR analyses show that both cell types express glucocorticoid receptor (GR; the target for DEX action), ruling out receptor expression as a causal factor in the lack of DEX-responsiveness. GRs function as ligand-activated transcription factors, so we simultaneously analyzed DEX-induced transcriptional responses using microarray analyses; these substantiated the histological findings, with limited gene expression changes in DEX-treated OPCs and oligodendrocytes. With identical treatment, microglial cells showed profound and global changes post-DEX addition; an unexpected finding was the identification of the transcription factor Olig1, a master regulator of myelination, as a DEX responsive gene in microglia. Our data indicate that CS-induced myelination delays are unlikely to be due to direct drug action on OPCs or oligodendrocytes, and may occur secondary to alterations in other neural cells, such as the immune component. To the best of our knowledge, this is the first comparative molecular and cellular analysis of CS effects in glial cells, to investigate the targets of this major class of anti-inflammatory drugs as a basis for myelination deficits.British Neuro-pathological Society, North Staffordshire Medical Institute, and The University of Nottingham

Normal vs. Inverted Hierarchy in Type I Seesaw Models

We demonstrate that, for every grand unified model based on a conventional type I seesaw mechanism leading to a normal light neutrino mass hierarchy, one can easily generate a corresponding model with an inverted hierarchy which yields the same neutrino oscillation parameters. However, the latter type model has several unattractive instabilities which will disfavor any grand unified type I seesaw model, if an inverted neutrino mass hierarchy is observed experimentally. This should be contrasted with the softly-broken $L_e - L_\mu - L_\tau$ flavor symmetry models which are eliminated, if the data favors a normal mass hierarchy.Comment: 10 pages including 1 figure; improved version with additional illustration of results for whole class of models with charged lepton mass matrices diagonal in flavor space; accepted for publication in Physics Letters

Reduced RNA turnover as a driver of cellular senescence

Accumulation of senescent cells is an important contributor to chronic inflammation upon aging. The inflammatory phenotype of senescent cells was previously shown to be driven by cytoplasmic DNA. Here, we propose that cytoplasmic double-stranded RNA has a similar effect. We find that several cell types driven into senescence by different routes share an accumulation of long promoter RNAs and 3' gene extensions rich in retrotransposon sequences. Accordingly, these cells display increased expression of genes involved in response to double stranded RNA of viral origin downstream of the interferon pathway. The RNA accumulation is associated with evidence of reduced RNA turnover, including in some cases, reduced expression of RNA exosome subunits. Reciprocally, depletion of RNA exosome subunit EXOSC3 accelerated expression of multiple senescence markers. A senescence-like RNA accumulation was also observed in cells exposed to oxidative stress, an important trigger of cellular senescence. Altogether, we propose that in a subset of senescent cells, repeat-containing transcripts stabilized by oxidative stress or reduced RNA exosome activity participate in driving and maintaining the permanent inflammatory state characterizing cellular senescence