5 research outputs found
Signal stability of Cy3 and Cy5 on antibody microarrays
BACKGROUND: The antibody microarray technique is a newly emerging proteomics tool for differential protein expression analyses that uses fluorescent dyes Cy 3 and Cy 5. Environmental factors, such as light exposure, can affect the signal intensity of fluorescent dyes on microarray slides thus, it is logical to scan microarray slides immediately after the final wash and drying processes. However, no research data are available concerning time-dependent changes of fluorescent signals on antibody microarray slides to this date. In the present study, microarray slides were preserved at -20°C after regular microarray experiments and were rescanned at day 10, 20 and 30 to evaluate change in signal intensity. RESULTS: Fluorescent intensities of microarray spots were detected using a confocal laser scanner after the experiment at day 0, and re-examined at day 10, 20 and 30, respectively. Fluorescent intensities of rescanned microarray spots did not show significant changes when compared with those scanned immediately after standard microarray experiments. CONCLUSION: Microarray slides can be preserved and rescanned multiple times using a confocal laser scanner over a period of days or weeks
Study of the bronchoscopic evaluation of patients undergoing bronchoscopy in respiratory medicine department
Background: Bronchoalveolar lavage or BAL is a minimally invasive procedure that involves instillation of sterile normal saline into a subsegment of the lung, followed by suction and collection of the instillation for analysis. This procedure is typically facilitated by the introduction of a flexible bronchoscope into a sub-segment of the lung. The purpose of this study was to investigate the pathological variations in the tracheobronchial tree in various respiratory diseases.
Methods: The study evaluated the bronchoscopic profile of 150 patients who underwent flexible bronchoscopy in the respiratory medicine department of MGM Medical College in Aurangabad, India from October 2020 to September 2022.
Results: The majority of the patients were males (65%) and above the age of 50. The most common reason for bronchoscopy was radiological opacity (80% of cases), followed by diffuse pulmonary infiltration (10%). The study found that bronchoscopy was useful in obtaining samples for diagnosis of various respiratory diseases, including malignancies, tuberculosis, bacterial pneumonia, and hematological malignancies. The study also noted the therapeutic utility of bronchoscopy in managing inoperable stenosis of the central airways.
Conclusions: The authors concluded that bronchoscopy is an important diagnostic and therapeutic tool for the management of pulmonary diseases
Study of the clinical profile and treatment outcome of pulmonary and extrapulmonary tuberculosis patients coming to respiratory medicine OPD
Background: The major risk factors for development of tuberculosis are extremes of age, malnutrition, overcrowding and immune deficient states like AIDS, diabetes mellitus, measles, whooping cough and leukaemia. Chronic malaria and worm infestation are less important risk factors. Tobacco smoking, high alcohol consumption, corticosteroid therapy and immunosuppressive drug therapy reduce body defences there by increasing the susceptibility to tuberculous infection. This study was undertaken to analyse the various clinical presentations in patients with pulmonary tuberculosis and extra pulmonary tuberculosis.
Methods: This was a retrospective study of patients from October 2020 to September 2022 (2 years). Data collected from 300 patients who were diagnosed with pulmonary and extrapulmonary tuberculosis visited. The subjects were divided into subjects who had pulmonary TB (PTB), and who had extrapulmonary TB (EPTB).
Results: The most common systemic disorders accompanying EPTB were DM (n=11; 8.8%) and chronic renal failure (n=8; 6.4%); while DM (n=16; 9.1%) and chronic obstructive pulmonary disease (n=13; 7.4%) were those detected frequently in PTB group.
Conclusions: Present study showed higher percentage of males and greater predominance of pulmonary TB. It is also observed high percentage extrapulmonary TB. High percentage of positive treatment outcomes with fewer relapses and failures was observed in this study. A high positive treatment outcome noted may be attributed to the availability of specialist doctors and diagnostic facilities in the tertiary care centre
Prevalence of obstructive airway disease in pulmonary function tests of patients visiting respiratory medicine out patient department
Background: The recognition of obstructive airway disease as a public health problem, however, has failed to keep pace with its increasing impact on health-care resources. This study is conducted to evaluate the prevalence of obstructive airway disease in pulmonary function tests (PFT) of patients visiting respiratory medicine out patient department (OPD).
Methods: This is a retrospective study of patients from October 2020 to September 2022 (2 years), on 80 subjects reporting to respiratory medicine OPD, MGM medical college, Aurangabad. Data collected from 80 subjects who were diagnosed with obstructive airway disease in PFT.
Results: The study population had a COPD prevalence of 16 (19.7%). GOLD criterion revealed that 54.2% (9/16) of COPD patients had mild COPD. This research showed no evidence of very severe COPD. In terms of gender distribution, 8 (16.6%) of 46 males and 7 (19.5%) of 34 women had COPD. However, there was no statistically significant (p=0.167) difference in COPD prevalence between sexes. Furthermore, univariable analysis revealed no significant differences in marital status (p=0.836), co-morbidities (p=0.541), family size (p=0.535), educational status (p=0.827), employment status (p=0.643), and medical visits (p=0.366) between persons with and without COPD.
Conclusions: According to the findings of this research, the variables that increase a person's likelihood of developing COPD are as follows: advancing age, prolonged exposure to smoke from biomass burning, tobacco use, and inadequate ventilation in the kitchen