FUS-DDIT3 Prevents the Development of Adipocytic Precursors in Liposarcoma by Repressing PPARγ and C/EBPα and Activating eIF4E

FUS-DDIT3 is a chimeric protein generated by the most common chromosomal translocation t(12;16)(q13;p11) linked to liposarcomas, which are characterized by the accumulation of early adipocytic precursors. Current studies indicate that FUS-DDIT3- liposarcoma develops from uncommitted progenitors. However, the precise mechanism whereby FUS-DDIT3 contributes to the differentiation arrest remains to be elucidated. METHODOLOGY/PRINCIPAL FINDINGS: Here we have characterized the adipocyte regulatory protein network in liposarcomas of FUS-DITT3 transgenic mice and showed that PPARgamma2 and C/EBPalpha expression was altered. Consistent with in vivo data, FUS-DDIT3 MEFs and human liposarcoma cell lines showed a similar downregulation of both PPARgamma2 and C/EBPalpha expression. Complementation studies with PPARgamma but not C/EBPalpha rescued the differentiation block in committed adipocytic precursors expressing FUS-DDIT3. Our results further show that FUS-DDIT3 interferes with the control of initiation of translation by upregulation of the eukaryotic translation initiation factors eIF2 and eIF4E both in FUS-DDIT3 mice and human liposarcomas cell lines, explaining the shift towards the truncated p30 isoform of C/EBPalpha in liposarcomas. Suppression of the FUS-DDIT3 transgene did rescue this adipocyte differentiation block. Moreover, eIF4E was also strongly upregulated in normal adipose tissue of FUS-DDIT3 transgenic mice, suggesting that overexpression of eIF4E may be a primary event in the initiation of liposarcomas. Reporter assays showed FUS-DDIT3 is involved in the upregulation of eIF4E in liposarcomas and that both domains of the fusion protein are required for affecting eIF4E expression. CONCLUSIONS/SIGNIFICANCE: Taken together, this study provides evidence of the molecular mechanisms involve in the disruption of normal adipocyte differentiation program in liposarcoma harbouring the chimeric gene FUS-DDIT3.Research in ISG group is supported partially by FEDER and by MEC (SAF2006-03726), Junta de Castilla y León (CSI03A05), FIS (PI050087, PI050116), Fundación de Investigación MMA, Federación de Cajas de Ahorro Castilla y León (I Convocatoria de Ayudas para Proyectos de Investigación Biosanitaria con Células Madre), CDTEAM project (CENIT-Ingenio 2010) and MEC Consolider-Ingenio 2010 (Ref. CSD2007-0017).Research in ISG group is supported partially by FEDER and by MEC (SAF2006-03726 and PETRI N° 95-0913.OP), Junta de Castilla y León (CSI03A05), FIS (PI050087, PI050116), Fundación de Investigación MMA, Federación de Cajas de Ahorro Castilla y León (I Convocatoria de Ayudas para Proyectos de Investigación Biosanitaria con Células Madre), CDTEAM project (CENIT-Ingenio 2010) and MEC Consolider-Ingenio 2010 (Ref. CSD2007-0017). MSM is supported by the Ramon y Cajal Scientific Spanish Program, Fondo Investigacion Sanitaria (FIS PI04-1271), Junta de Castilla y León (SA085A06) and Fundación Manuel Solorzano, University of Salamanca.Peer reviewe

Corrigendum to ‘Dietary aflatoxin B1 (AFB1) reduces growth performance, impacting growth axis, metabolism, and tissue integrity in juvenile gilthead sea bream (Sparus aurata)’. Aquaculture, volume 533, 25 February 2021, 736189

The authors regret the errors in a few table references within the text. Specifically, it should reads as follows within the following subsections/ page: 3.2. Blood analysis (page 5)info:eu-repo/semantics/publishedVersio

Digestive enzymes indicating nutritional condition in octopus paralarvae Octopus vulgaris Cuvier, 1797

Experiments were conducted to evaluate the effect of different diets in Octopus vulgaris Cuvier, 1797 paralarvae. Diets were composed of different combinations of Artemia salina (L., 1758), Moina salina Daday, 1888 and crab zoea, as well as artificial food. Wet weight of the paralarvae was recorded. Protease and trypsin activity were determined in a number of individuals to assess changes in the digestive use of food. Results show that enzyme secretion was affected both by age and the type of food. It is deduced that protease activity can be used as spawning quality index, and that trypsin activity gives information about whether paralarvae are feeding.El cultivo del pulpo común Octopus vulgaris Cuvier, 1797 no plantea problemas en cuanto a la adaptación de ejemplares a cautividad, engorde de juveniles, maduración sexual y puesta. Sin embargo, la tasa de mortalidad larvaria es muy elevada. La determinación de los cambios en las actividades enzimáticas durante el desarrollo larvario puede ser útil para establecer el momento óptimo del destete y para comprender la dependencia de la larva de fuentes de enzimas exógenas. Con el objetivo de determinar las capacidades digestivas de las paralarvas de pulpo se han llevado a cabo experimentos para evaluar el efecto en ellas de distintas dietas. éstas estaban compuestas por diferentes combinaciones de Artemia salina (L., 1758), Moina salina Daday, 1888 y zoeas de crustáceos, así como microcápsulas de dieta artificial. Determinado el peso húmedo de las paralarvas, se midió la actividad de proteasas y tripsina en ejemplares individuales para valorar la capacidad digestiva sobre los distintos tratamientos. Los resultados mostraron que la secreción enzimática estaba influida por la edad y el tipo de alimentación. Se deduce, pues, que las proteasas pueden ser usadas como índice de calidad de la puesta y la tripsina como indicador de que las paralarvas se están alimentando.Instituto Español de Oceanografí

Effect of amino acid supplementation and stress on expression of molecular markers in meagre (Argyrosomus regius)

The objectives of this work were: 1) develop of molecular stress biomarkers obtaining sequence data of different transcripts, 2) study the molecular stress response through the expression quantification of key gene involved in it, and 3) assess the effects of dietary amino acid additives on stress response in meagre meagres (Argyrososmus regius). Fish batches were fed two experimental diets with tryptophan (Trp) or aspartate (Asp) added for seven days. Before sampling fish were submitted to confinement/netting stress during 1 h, except control fish. Therefore fish were sampled before and after stress (1 h and 6 h post-stress). The sampling consisted of blood and tissues (brain, hypophysis and liver). Several gene expressions related to the stress response were measured in those tissues, and the cloning of corticotropin-releasing hormone (crh), corticotropin-releasing hormone binding protein (crh-bp), and thyrotropin-releasing hormone (trh) has been reported in meagre for the first time. In fact, fish fed an additional Asp diet did not present any sl, prl and gh expression changes, as for the control group. Contrarily, the Trp diet altered the prl and gh expressions after stress. For crh and crh-bp expressions, no significant differences were detected within the Asp diet hence that amino acid improved the stress response. However, Asp feeding, but not Trp, enhanced pomc-a expression after stress. Hsp70 expression varied for every treatment, including the control feeding, indicating a late response at 6 h post-stress sampling, where both Asp and Trp treatments increased these expressions significantly. Concluding, the response of molecular stress markers to amino acid enriched diets was diverse. The stressor did not change significantly the relative expression of most analyzed genes for control feeding groups, though the Asp supplemented diet was more effective for attenuating molecular markers than the Trp one.info:eu-repo/semantics/publishedVersio

Preliminary study of the adaptation of Senegalese sole Solea senegalensis Kaup, 1858 to different salinities

Osmoregulatory and metabolic effects of adaptation to different water salinities (5, 15, 25, 42 and 55) were assessed during a period of 14 days in juveniles of Senegalese sole Solea senegalensis Kaup, 1858. The results showed a good capacity to adapt within the range of water salinities tested. Gill Na+,K+-ATPase activity showed a direct lineal relationship with regard to water salinity, whereas kidney Na+,K+-ATPase activity did not change. Plasma osmolality and ions levels did not present differences among the groups. Plasma cortisol, glucose and lactate were higher in the group maintained in the highest water salinity.El crecimiento del lenguado senegalés Solea senegalensis Kaup, 1858 está influido por diferentes variables, y una de ellas es la salinidad ambiental. En este trabajo, se estudian los efectos osmorreguladores y metabólicos de la aclimatación a distintas salinidades (5, 15, 25, 42 y 55) durante un periodo de 14 días en juveniles de esta especie. La actividad Na+,K+-ATPasa branquial presentó una relación lineal directa respecto a la salinidad ambiental, mientras que la actividad Na+,K+-ATPasa renal no experimentó variación alguna. Ni la osmolalidad ni los niveles de iones plasmáticos analizados mostraron diferencias significativas entre los diferentes grupos. El cortisol y los niveles de glucosa y lactato plasmático aumentaron en los ejemplares adaptados a la mayor salinidad. Los resultados mostraron una buena capacidad de aclimatación en el rango de salinidades ambientales analizado.Instituto Español de Oceanografí

GENDER EQUITY LANDSCAPE IN HIGHER EDUCATION DURING PANDEMIC YEARS: LINKING ORGANIZATIONAL PRACTICES TO TEACHERS’ MORALE AND PRODUCTIVITY

This study sought to ascertain how organizational strategies affected gender equity in a teacher’s morale and productivity among male, female, and nonbinary faculty at Jose Rizal Memorial State University, utilizing a descriptive correlational research design with the use of standardized tests. The frequency count, percent, mean, Chi-square, and Pearson R statistical tests were used to total, tabulate, and further analyze and interpret the results. The findings showed a substantial association, demonstrating how organizational practices have an effect on a teacher's level of morale. The organization may implement leadership development programs to prepare upcoming deans or associate deans for their positions. Organizational practices and faculty production in research were not significantly correlated, however there was a strong correlation between organizational practices and faculty productivity in instruction and community services. Gender equity within an organization can stimulate improvements in instruction and performance in extension but does not lead to research productivity among the faculty of the university. Thus, the deans and associate deans may include in their PPMP the purchase of classroom supplies, equipment, and other audio-visual aids and projection equipment. Furthermore, a staff may be hired in the college to assist the faculty with encoding, designing, and developing instructional materials. On the other hand, gender equality among university teachers does not result in increased productivity in research. The college may conduct action research to explore the factors that may affect the research productivity of the male, female, and nonbinary faculty. Furthermore, the proposed "Action Plan for Monitoring and Evaluating Gender Equity in Institutional Work Plans and Activities" may be utilized to sustain gender equity in educational institutions

Population genomics of domestic and wild yeasts

The natural genetics of an organism is determined by the distribution of sequences of its genome. Here we present one- to four-fold, with some deeper, coverage of the genome sequences of over seventy isolates of the domesticated baker's yeast, _Saccharomyces cerevisiae_, and its closest relative, the wild _S. paradoxus_, which has never been associated with human activity. These were collected from numerous geographic locations and sources (including wild, clinical, baking, wine, laboratory and food spoilage). These sequences provide an unprecedented view of the population structure, natural (and artificial) selection and genome evolution in these species. Variation in gene content, SNPs, indels, copy numbers and transposable elements provide insights into the evolution of different lineages. Phenotypic variation broadly correlates with global genome-wide phylogenetic relationships however there is no correlation with source. _S. paradoxus_ populations are well delineated along geographic boundaries while the variation among worldwide _S. cerevisiae_ isolates show less differentiation and is comparable to a single _S. paradoxus_ population. Rather than one or two domestication events leading to the extant baker's yeasts, the population structure of _S. cerevisiae_ shows a few well defined geographically isolated lineages and many different mosaics of these lineages, supporting the notion that human influence provided the opportunity for outbreeding and production of new combinations of pre-existing variation

Enhanced triplet superconductivity in next generation ultraclean UTe2

The spin-triplet superconductor UTe$_2$ exhibits a myriad of exotic physical phenomena, including the possession of three distinct superconducting phases at ambient pressure for magnetic field $\mu_0 H \leq$ 40 T aligned in certain orientations. However, contradictory reports between studies performed on UTe$_2$ specimens of varying quality have severely impeded theoretical efforts to understand the microscopic properties of this material. Here, we report high magnetic field measurements on a new generation of ultraclean UTe$_2$ specimens, which possess enhanced superconducting critical temperatures and fields compared to previous sample generations. Remarkably, for $H$ applied close to the hard magnetic $b$ direction, we find that the angular extent of magnetic field-reinforced superconductivity is significantly increased in these high purity crystals. This suggests that, in proximity to a field-induced metamagnetic transition, the enhanced role of magnetic fluctuations - that are strongly suppressed by disorder - is likely responsible for tuning UTe$_2$ between two distinct spin-triplet superconducting phases. Our results reveal a strong sensitivity to crystalline disorder of the field-reinforced superconducting state of UTe$_2$