Phenolic Compounds from Moroccan <i>Retama monosperma</i> L. Boiss, <i>Berberis vulgaris</i> L. and <i>Ricinus communis</i> L.: Characterization, Antioxidant Activity and Performance Criteria of the Validated Method Using UHPLC/DAD/ESI-MS

Components of medicinal plants have many pharmacological activities, including antioxidant activity, playing an important role in limiting oxidative stress that can cause several damages. This paper characterizes polyphenols of Retama monosperma L., Berberis vulgaris L. and Ricinus communis L. plant extracts and evaluates their antioxidant activity by DPPH, conjugated diene and TBARS assay. To ensure the quality of analytical results, this paper presents performance criteria of the validated method using UHPLC/DAD/ESI-MS. Regarding method validation, the results confirm different used tests and evaluate detection and quantification limits. Concerning the characterization and study of antioxidant activity, realized testing showed that R. monosperma is rich in isoflavone, flavone and flavonol. For R. communis, we notice the presence of rutin as a major compound. Meanwhile, B. vulgaris contains significant amounts of gallic acid and p-coumaric acid. These plant extracts have high antioxidant activity due to the presence of phenolic compounds

Effect of Adding Thymus leptobotrys on Extra Virgin Olive Oil’s Quality and Physicochemical Parameters

The present study aimed to evaluate the changes in extra virgin olive oil (EVOO) after enrichment treatments with Thymus leptobotrys, a Moroccan endemic aromatic plant. The EVOO sample was supplemented with two materials obtained from the aerial parts of T. leptobotrys: powder (2.5 and 5%) or essential oil (100 and 200 ppm). The chemical composition analysis of T. leptobotrys extracts (essential oil and powder methanolic extract) allowed the determination of its phenolic profile. Quality parameters (acidity, peroxide value, and specific extinction coefficients), color, fatty acids composition, minor components (pigments and phenol), and Rancimat oxidative stability of the control and enriched oils were measured. Rosmarinic acid and thymusin were the main identified compounds in the powder; thymusin was identified for the first time in the essential oil where it was predominant. Generally, the enrichments did not downgrade the oil from its initial category (EVOO), according to quality indexes. Adding T. leptobotrys to EVOO, especially the essential oil, enriched its phenolic profile by allowing the migration of thymusin, a lipophile flavone, without affecting its quality parameters or color

Kinetics, mechanism studies and antibacterial activity of pharmaceutical Sulfaguanidine under light irradiation in aqueous solution

International audienceThe presence of pharmaceutical compounds in the environment worries several researchers for several years since their existence in aquatic systems leads to major environmental problems. These compounds are a unique category of pollutants and considered emerging contaminants in water supply systems. They have been found in groundwater and even in drinking water at concentrations ranging from ng to µg/L.In the present work, we investigated the photochemical behavior of a sulfonamide antibiotic, namely sulfaguanidine (SGD) in aqueous solutions as a function of pH and we examined the inhibitory activity of photoproducts SDG against E coli BLSE and S. aureus using the disc diffusion method, determination of minimum inhibitory concentration and cell viability percentage. Quantum yields were measured at 254 and 302 nm during irradiation in absence and presence of oxygen). In aerated conditions, they were evaluated to 2.3 x 10−2 and 2.5 x 10−3 respectively. However, the degradation process is not completely inhibited in the presence of oxygen due to sulfaguanidine photoreactivity, which is in agreement with the involvement of the singlet as well as the triplet excited states.Several photoproducts were elucidated using liquid chromatography coupled with positive mode mass spectroscopy LC/MS/ESI +. For bacterial activity, these photoproducts shows more toxic that the Sulfaguanidine and exhibited a higher inhibitory activity against E coli and S. aureus cells