Diet of the Italian hare ( Lepus corsicanus ) in a semi-natural landscape of southern Italy

The food habits of the endangered Italian hare have not received adequate attention from researchers. In this study, the diet composition of this species and its seasonal variation were assessed by analysing faecal pellets in a semi-natural landscape in the south of Italy. The results showed that hares feed on 62 species of plants during the year, with a conspicuous presence of herbaceous ones (e.g., Trifolium pratense , Brachypodium sylvaticum , Festuca arundinacea ) as these occurred at high frequencies in most of the faecal samples. In spring, diet composition was characterised by a high percentage of Graminaceae ( > 37%). In the other seasons, hares also included fruits (e.g., Prunus spinosa , Pyrus piraster , Malus sylvestris ), which, in autumn, accounted for > 27%. There were significant differences among seasons (p < 0.001) in terms of Margalef ’ s richness, Shannon diversity, and Buzas and Gibson ’ s evenness. The smallest values of richness and diversity were observed in spring. Dietary overlap was low between spring and the other seasons; conversely, there was substantial overlap ( > 70%) in the diets during the other seasons with a more pronounced similarity between summer and autumn (S ø rensen, C s = 0.80; Morisita-Horn, C MH = 0.73)

Therapeutic efficacy of milbemycin oxime/praziquantel oral formulation (Milbemax®) against Thelazia callipaeda in naturally infested dogs and cats

BACKGROUND: Over the last few decades, canine and feline thelaziosis caused by Thelazia callipaeda eye worms has gained the attention of the veterinary community due to the spread of this ocular infestation in geographical areas previously regarded as non endemic. The therapeutic efficacy of milbemycin oxime/praziquantel tablets (Milbemax®) against T. callipaeda was tested in naturally infested dogs and cats. METHODS: From January 2009 to July 2011 a placebo controlled and randomized field study was conducted in T. callipaeda endemic areas of Switzerland (CH) and Italy (ITA) involving client-owned animals. Dogs (n = 56) and cats (n = 31) were physically examined at enrolment Day 0 (D0) and twice afterwards (D7 and D14). Infested animals were orally treated with Milbemax® or with placebo tablets on D0 and, if an animal was found still infested with T. callipaeda, also on D7. On D14 nematodes were flushed from the conjunctiva, identified and counted. RESULTS: Out of 56 dogs, 43 were included in the statistical analysis, whereas 13 were excluded because the products under investigation were not administered with food, as required by the label. On D7 and D14, 72.7% and 90.9% of treated dogs were eye worm free, whereas in the placebo group 95.2% and 76.2% still harbored nematodes, resulting in a mean percentage worm count reduction for the Milbemax® group of 86.1% and 96.8%, respectively. Both results were significantly higher (p = 0.0001) than the placebo group. Out of the 31 cats included in the study at D7 and D14, 53.3% and 73.3% treated with Milbemax® were free of T. callipaeda, while 81.3% and 73.3 in the placebo group were still harbouring eye worms, resulting in a mean percentage worm count reduction for the treated group of 62.2% and 80.0%, respectively. Both results were significantly higher (p = 0.0106 and p = 0.0043) than the placebo group. CONCLUSIONS: The commercial formulation of milbemycin oxime at the minimal dose of 0.5 mg/kg and 2 mg/k in dogs and cats, respectively, showed a high therapeutic efficacy in curing T. callipaeda infestations. The advantages of an oral application are additionally increased by the large spectrum of activity of praziquantel and milbemycin oxime against Cestodes and Nematodes infesting dogs and cats

Breeding population of Black Stork, Ciconia nigra, in Italy between 1994 and 2016

The Black Stork Ciconia nigra, following an expansion on European scale, started breeding in Italy in 1994 with one pair in the Piedmont Region and one in the Calabria Region. Since then, the breeding pairs established in Italy have progressively increased up to 18 in 2016, and they are currently in Piedmont, Lazio, Campania, Molise, Apulia, Basilicata and Calabria. However, the number of breeding pairs could be higher than 20, as indicated by records and observations of adults and juveniles, during the breeding period in potentially suitable nesting areas. Despite the low population density in Italy, the trend in the breeding population in Northwest and in Southern Central regions seems to show a slight and high increase respectively. Productivity, breeding success and fledging rate have been considered and analysed. A difference between the two macro areas has been found in the choice of nesting sites, which is on trees for Northwest couples, and cliffs for Southern Central couples. It is necessary to further explore the reason why the small Northwest population does not show any increase and range expansion compared to the Southern Central one

Diurnal timing of nonmigratory movement by birds: the importance of foraging spatial scales

Timing of activity can reveal an organism's efforts to optimize foraging either by minimizing energy loss through passive movement or by maximizing energetic gain through foraging. Here, we assess whether signals of either of these strategies are detectable in the timing of activity of daily, local movements by birds. We compare the similarities of timing of movement activity among species using six temporal variables: start of activity relative to sunrise, end of activity relative to sunset, relative speed at midday, number of movement bouts, bout duration and proportion of active daytime hours. We test for the influence of flight mode and foraging habitat on the timing of movement activity across avian guilds. We used 64 570 days of GPS movement data collected between 2002 and 2019 for local (non‐migratory) movements of 991 birds from 49 species, representing 14 orders. Dissimilarity among daily activity patterns was best explained by flight mode. Terrestrial soaring birds began activity later and stopped activity earlier than pelagic soaring or flapping birds. Broad‐scale foraging habitat explained less of the clustering patterns because of divergent timing of active periods of pelagic surface and diving foragers. Among pelagic birds, surface foragers were active throughout all 24 hrs of the day while diving foragers matched their active hours more closely to daylight hours. Pelagic surface foragers also had the greatest daily foraging distances, which was consistent with their daytime activity patterns. This study demonstrates that flight mode and foraging habitat influence temporal patterns of daily movement activity of birds.We thank the Nature Conservancy, the Bailey Wildlife Foundation, the Bluestone Foundation, the Ocean View Foundation, Biodiversity Research Institute, the Maine Outdoor Heritage Fund, the Davis Conservation Foundation and The U.S. Department of Energy (DE‐EE0005362), and the Darwin Initiative (19-026), EDP S.A. ‘Fundação para a Biodiversidade’ and the Portuguese Foundation for Science and Technology (FCT) (DL57/2019/CP 1440/CT 0021), Enterprise St Helena (ESH), Friends of National Zoo Conservation Research Grant Program and Conservation Nation, ConocoPhillips Global Signature Program, Maryland Department of Natural Resources, Cellular Tracking Technologies and Hawk Mountain Sanctuary for providing funding and in-kind support for the GPS data used in our analyses

Tracking data highlight the importance of human-induced mortality for large migratory birds at a flyway scale

Human-induced direct mortality affects huge numbers of birds each year, threatening hundreds of species worldwide. Tracking technologies can be an important tool to investigate temporal and spatial patterns of bird mortality as well as their drivers. We compiled 1704 mortality records from tracking studies across the African-Eurasian flyway for 45 species, including raptors, storks, and cranes, covering the period from 2003 to 2021. Our results show a higher frequency of human-induced causes of mortality than natural causes across taxonomic groups, geographical areas, and age classes. Moreover, we found that the frequency of human-induced mortality remained stable over the study period. From the human-induced mortality events with a known cause (n = 637), three main causes were identified: electrocution (40.5 %), illegal killing (21.7 %), and poisoning (16.3 %). Additionally, combined energy infrastructure-related mortality (i.e., electrocution, power line collision, and wind-farm collision) represented 49 % of all human-induced mortality events. Using a random forest model, the main predictors of human-induced mortality were found to be taxonomic group, geographic location (latitude and longitude), and human footprint index value at the location of mortality. Despite conservation efforts, human drivers of bird mortality in the African-Eurasian flyway do not appear to have declined over the last 15 years for the studied group of species. Results suggest that stronger conservation actions to address these threats across the flyway can reduce their impacts on species. In particular, projected future development of energy infrastructure is a representative example where application of planning, operation, and mitigation measures can enhance bird conservation

First contribute to the characterization of coat in Lepus corsicanus and Lepus europaeus by colorimetric determinations

Thetrial wascarriedout on12sympatricadult females: 6Italianhares(Lepus Thetrial wascarriedout on12sympatricadult females: 6Italianhares(Lepus trial was carried out on 12 sympatric adult females: 6 Italian hares (Lepus corsicanus) and 6 Brown hares (Lepus europaeus). Colour characteristics were studied by CIEL*a*b* methodonthefollowinganatomicalregions:forehead,subocularpatch,nape,withers,rump,hindleg, onthefollowinganatomicalregions:forehead,subocularpatch,nape,withers,rump,hindleg, on the following anatomical regions: forehead, subocular patch, nape, withers, rump, hind leg, knee, and belly. Differences between means were tested by t test. The most striking differences were observed in a* and in C indexes. Italian hares resulted more reddish in knee (5.22 vs. 3.93, P≤0.001) and in hind leg (2.96 vs. 1.34, P≤0.01) and less reddish (P≤0.001) in the regions of subocular patch (3.35 vs. 5.34), nape (3.24 vs. 6.05) and withers (3.25 vs. 4.81) than Brown hare. Colour saturation was lower (nearer to the grey) in the regions forehead (4.78 vs. 6.41 P P≤0.01),subocularpatch(8.91 ), subocular patch(8.91 (8.91 vs. 11.24, P≤0.05), nape (4.82 vs. 10.56, P≤0.001), withers (4.63 vs. 8.91, P≤0.001), and rump (5.81 vs. 8.30, P≤0.05) and higher in the regions hind leg (11.07 vs. 4.46, P≤0.001), knee (15.45 vs. 11.14, P≤0.001), and belly (3.30 vs. 2.15, P≤0.05) in Italian hare than in Brown hare. These differences are consistent with colour pattern of coat describedinliterature. described in literature

Postnatal growth of Brown hare (Lepus europaeus) in a South Italy rearing centre

The aim of this work is to describe the postnatal growth performance (period from birth to weaning) in Brown hare (Lepus europaeus) leverets in a rearing centre of Southern Italy. Three morpho- metric variables (body weight, hind foot length and ear length) were measured weekly from birth to wean- ing (at 21 days) in 78 leverets. Data were analysed by proc GLM (SAS) considering the effect of sex and birth period (1. January-February, 2. March-April, and 3. May-June). Birth period influenced significantly live weight at 14 14th day of life, lower inperiod2thanin3(553vs.607g;P period 2 than in 3(553vs.607g;P (553 vs. 607 g; P≤0.05) and hind foot length at birth, shorter in period 1 compared to the period 2 and 3 (4.4 vs. 4.7 and 4.8 cm; P≤0.05), and at weaning, shorter in period 1 than in 3 (8.6 vs. 9.6 cm; P≤0.05). Leveretsincreasedwithin21daystheirbodyweight6.26,6.76, Leverets increased within 21 days their body weight 6.26,6.76, 6.26, 6.76, and 6.97 foldinperiod1,2,and3,respectively.Maximumgrowthspeed(over40g/dinallbirthperiods)was in period 1, 2, and 3, respectively. Maximum growth speed (over 40g/d in all birth periods) was reached at the 3rd week. These results were consistent with the best performances described in literature for this species. Our data can be useful to evaluate the development of leverets in other rearing centres in Southern Italy. Furthermore, our data can be helpful for aging young Brown hares captured in the field, for example inthosestudiesaimedtoanalyzethecompetitionbetweenBrownhareandendemicItalianhare in those studies aimed to analyze the competition between Brown hare and endemic Italian hare (Lepuscorsicanus)inMediterraneanecosystems. Lepus corsicanus)inMediterraneanecosystems. in Mediterraneanecosystems. Mediterranean ecosystems.

Wild Birds as biological indicators of environmental pollution: biotyping and antimicrobial resistance patterns of Escherichia coli isolated from Audouin's gulls (Larus Audouinii) living in the Bay of Gallipoli (Italy)

E. Coli biotyping and antimicrobial succeptibility tests were performed on fortyeight cloacal swabs collected from a popoulation of Audouin's gulls ((Larus Audouinii) living in the Bay of Gallipoli (Lecce, Italy). The aim was to assess the pathogenic potential of the strains the gulls carry and shed into the environment and to gain a better understanding of the microbial pollution of the aera they live in

First Report on a Cliff-Nesting Pair of Black Storks (Ciconia nigra Linnaeus, 1758) and Their Nestlings

The black stork is an endangered species in many countries, due to its low adaptability to environmental changes and its sensitivity to anthropogenic disturbances. In Italy, the most recent report on the species’ nesting sites lists only 36 pairs, of which 16 are in Basilicata, 7 are in Calabria, 4 are in Piedmont and Molise and 1 is in Campania. This study focuses for the first time on the behavior of a Ciconia nigra pair in the Basilicata region, where the species nests exclusively on cliffs rather than in trees, as is more frequent elsewhere. The video recordings were used to observe the species during the pre- and post-hatching periods and to refer to the 2012 nesting season, as in that year, the video recordings covered the entire reproductive period. In the pre-hatching phase, the activity for which most time is spent is brooding, which lasts on average 43′ in the morning and in evening and more than 49′ at midday. In the post-hatching phase, a large part of the recording period is spent on activities related to parental care. There were no moments of inactivity during this phase, the parents were frequently observed setting up the nest and preening themselves, while they were rarely seen in a huddled position. The alert activity was also very frequent, especially at midday. Our study has shown that the black stork, a shy and cautious species, may return to nest in increasing numbers given the development of the promising Lucanian nucleus, if attention is paid to the habitat quality and feeding areas, where human activities should be avoided unless absolutely necessary

Wild Birds as biological indicators of environmental pollution: biotyping and antimicrobial resistance patterns of Escherichia coli isolated from Audouin's gulls (Larus Audouinii) living in the Bay of Gallipoli (Italy)

E. Coli biotyping and antimicrobial succeptibility tests were performed on fortyeight cloacal swabs collected from a popoulation of Audouin's gulls ((Larus Audouinii) living in the Bay of Gallipoli (Lecce, Italy). The aim was to assess the pathogenic potential of the strains the gulls carry and shed into the environment and to gain a better understanding of the microbial pollution of the aera they live in