73 research outputs found
Metal oxide–chitosan based nanocomposite for cholesterol biosensor
Metal oxide [cerium oxide (NanoCeO2)]–chitosan (CH) nanocomposite film has been fabricated onto indium-tin-oxide (ITO) coated glass plate to immobilize cholesterol oxidase (ChOx) via physiosorption for cholesterol detection. Electrochemical studies reveal that the presence of NanoCeO2 in CH–CeO2 nanocomposite results in increased electroactive surface area for ChOx loading resulting in enhanced electron transport between ChOx and electrode. The ChOx/CH–NanoCeO2/ITO bioelectrode exhibits interesting characteristics such as detection range of 10–400 mg/dL, detection limit of 5 mg/dL, response time of 10 s, low Km value of 3.5 mg/dL and value of regression coefficient of 0.994
Nucleic acid immobilized polypyrrole–polyvinylsulphonate film for Mycobacterium tuberculosis detection
21-mer peptide acid nucleic acid (PNA) probe specific to 16s-23s rRNA spacer region of Mycobacterium tuberculosis has been covalently immobilized on polypyrrole-polyvinylsulphonate film electro-chemically deposited onto indium-tin-oxide (ITO) glass for detection of complementary target by monitoring guanine oxidation and redox indicators (methylene blue and ruthenium complex) up to 0.1 fmole, 0.1 attomole and 1.0 pmole, respectively within 30 s of hybridization time. The peptide nucleic acid immobilized polypyrrole-polyvinylsulphonate electrode can be used for hybridization detection with complementary sequence in heat-shocked genomic DNA and in serum samples containing genomic M. tuberculosis DNA up to 2.5 pg/mu l within about 60 min at 30 degrees C and can be used 8-9 times
Erratum to :Nucleic acid immobilized polypyrrole-polyvinylsulphonate film for Mycobacterium tuberculosis detection (vol 10, pg 821, 2008)
Erratum to :Nucleic acid immobilized polypyrrole-polyvinylsulphonate film for Mycobacterium tuberculosis detection (vol 10, pg 821, 2008
Cerium oxide-chitosan based nanobiocomposite for food borne mycotoxin detection
Cerium oxide nanoparticles (NanoCeO2) and chitosan (CH) based nanobiocomposite film deposited onto indium-tin-oxide coated glass substrate has been used to coimmobilize rabbit immunoglobin (r-IgGs) and bovine serum albumin (BSA) for food borne mycotoxin [ochratoxin-A (OTA)] detection. Electrochemical studies reveal that presence of NanoCeO2 increases effective electro-active surface area of CH-NanoCeO2/indium tin oxide (ITO) nanobiocomposite resulting in high loading of r-IgGs. BSA/r-IgGs/CH-NanoCeO2/ITO immunoelectrode exhibits improved linearity (0.25–6.0 ng/dl), detection limit (0.25 ng/dl), response time (25 s), sensitivity (18 μA/ng dl−1 cm−2), and regression coefficient (r2 ∼ 0.997)
Recent advances in cholesterol biosenso
Biosensors have recently gained much attention in the field of health care for the management of various important analytes in a biological system. The area achieved tremendous progress from the time when the first Clark electrode for measurement of glucose was realized. Advances in the biosensor design are appearing at a high rate as these devices play increasingly important roles in our daily lives. The increasing incidences of cardiovascular diseases and cardiac arrest are major cause of death of humans world over. One of the most important reasons is hypercholesterolemia, i.e. increased concentration of cholesterol in blood. Hence estimation of cholesterol level in blood is important in clinical applications. This re
view aims to highlight the recent advances in materials and techniques for cholesterol biosensor design and construction
Prospects of conducting polymers in biosensors
pplications of conducting polymers to biosensors have recently aroused much interest. This is because these molecular electronic materials offer control of different parameters such as polymer layer thickness, electrical properties and bio-reagent loading, etc. Moreover, conducting polymer based biosensors are likely to cater to the pressing requirements such as biocompatibility, possibility of in vivo sensing, continuous monitoring of drugs or metabolites, multi-parametric assays, miniaturization and high information density. This paper deals with the emerging trends in conducting polymer based biosensors during the last about 5 years
Prospects of conducting polymers in biosensors
pplications of conducting polymers to biosensors have recently aroused much interest. This is because these molecular electronic materials offer control of different parameters such as polymer layer thickness, electrical properties and bio-reagent loading, etc. Moreover, conducting polymer based biosensors are likely to cater to the pressing requirements such as biocompatibility, possibility of in vivo sensing, continuous monitoring of drugs or metabolites, multi-parametric assays, miniaturization and high information density. This paper deals with the emerging trends in conducting polymer based biosensors during the last about 5 years
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