Ergebnisse eines Zuchtexperiments zur Ermittlung der Effektivitaet der Selektion nach biochemischen Parametern oder Ultraschallmassen zur Verbesserung der Schlachtkoerperqualitaet beim Schwein

.Die Reduzierung des Fettansatzes war bei der direkten Selektion nach der Rueckenspeckdicke am groessten. Die Selektion aufgrund Ultraschallmessungen fuehrten zu einem etwas groesseren Anteil wertvoller Teilstuecke. Die Fleischbeschaffenheit war in den Selektionslinien - verglichen mit der Kontrollinie - am unguenstigsten. Tiere der Ultraschall-Selektionslinie zeigten die groesste Stressempfindlichkeit. Der Versuch umfasste 9 SelektionsgenerationenSIGLEAvailable from: Zentralstelle fuer Agrardokumentation und -information (ZADI), Villichgasse 17, D-53177 Bonn / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman

Adaptation of Mycobacterium smegmatis to an industrial scale medium and isolation of the mycobacterial porinMspA

The adaptation of the organism to a simple and cost-effective growth medium is mandatory in developing a process for large scale production of the octamericporinMspA, which is isolated from Mycobacterium smegmatis. A fermentation optimization with the minimal nutrients required for growth has been performed. During the fermentation, the iron- and ammonium chloride concentrations in the medium were varied to determine their impact on the observed growth rates and cell mass yields. Common antibiotics to control contamination were eliminated in favor of copper sulfate to reduce costs. MspA has been successfully isolated from the harvested M. smegmatisusing aqueous nOPOE (noctyloligooxyethylene) at 65°C. Because of the extraordinary stability of MspA, it is possible to denature and precipitate virtually all other proteins and contaminants by following this approach. To further purify the product, acetone is used for precipitation. Gel electrophoresis confirmed the presence and purity of MspA. A maximum of 840µg (via Bradford assay) of pure MspA per liter of the optimized simple growth medium has been obtained. This is a 40% increase with respect to the previously reported culture medium for MspA