Neglected isolated plantar dislocation of middle cuneiform : a case report

BACKGROUND: Four cases of plantar dislocation of middle cuneiform have been reported in the english literature. All of them were fresh cases and treated with open reduction. We are reporting a case of neglected plantar dislocation of middle cuneiform which was treated with excision. CASE PRESENTATION: A farmer presented with a painful plantar dislocation of middle cuneiform bone after 9 months of injury. The bone was deformed and was excised by a plantar incision. It resulted in painless foot with no disability. CONCLUSION: The neglected plantar dislocated middle cuneiform bone becomes deformed due to repeated weight bearing. The gap gets filled with Fibrous tissue. Excision of the cuneiform gives good results

Differential gene expression analysis in germinating and dormant teliospores of Tilletia indica using RNA seq approach

Karnal bunt of wheat is an important quarantine disease that interrupts India’s wheat trade in the international market. The whole transcriptome of germinating and dormant teliospores of Tilletia indica was performed using the RNA Seq approach to identify germination-related genes. Approximately 63 million reads were generated using the RNA sequencing by the Illumina NextSeq500 platform. The high-quality reads were deposited in NCBI SRA database (accession: PRJNA522347). The unigenes from the pooled teliospores were 16,575 having unigenes length of 28,998,753 bases. The high-quality reads of germinating teliospores mapped on to 21,505 predicted CDSs. 9,680 CDSs were common between dormant and germinating teliospores of T. indica. 11,825 CDSs were found to be in germinating teliospores while only 91 were unique in dormant spores of pathogen. The pathway analysis showed the highest number of pathways was found in germinating spores than dormant spores. The highest numbers of CDSs were found to be associated with translation (431 in number), transport and catabolism (340), signal transduction (326), and carbohydrate metabolism (283). The differential expression analysis (DESeq) of germinating and dormant teliospores showed that 686 CDS were up-regulated and 114 CDS were down-regulated in the germinating teliospores. Significant germination-related genes in the spores were validated using qPCR analysis. Ten genes viz. Ti3931, Ti6828, Ti7098, Ti7462, Ti7522, Ti 9289, Ti 8670, Ti 7959, Ti 7809,and Ti10095 were highly up-regulated in germinated teliospores which may have role in germination of spores.Further, these differentially expressed genes provide insights into the molecular events. This first study of transcriptome will be helpful to devise better management strategies to manage Karnal bunt disease

SRI-A Method for Sustainable Intensification of Rice Production with Enhanced Water Productivity

Climate change induced higher temperatures will increase crops’ water requirements. Every 10°C increase in mean temperature, results in 7% decline in the yield of rice crop. Hence, there is a need to develop water saving technologies in rice which consumes more than 50% of the total irrigation water in agriculture. System of Rice Intensification (SRI) is one such water saving rice production technology. Experiments were conducted at different locations in India including research farm of Directorate of Rice Research (DRR), Hyderabad, during 2005-10 to assess the potential of SRI in comparison to normal transplanting/Standard Planting (NTP/SP) under flooded condition. SRI recorded higher grain yield (6 to 65% over NTP) at majority of locations. Long term studies clearly indicated that grain yield was significantly higher (12-23% and 4-35% over NTP in Kharif and Rabi seasons, respectively) in SRI (with organic+inorganic fertilizers) while the SRI (with100% organic manures), recorded higher yield (4-34%) over NTP only in the Rabi seasons. Even though, SRI resulted in higher productivity, the available nutrient status in soil was marginally higher (10, 42 and 13% over NTP for N, P and K, respectively) at the end of four seasons. There was a reduction in the incidence of pests in SRI and the relative abundance of plant parasitic nematodes was low in SRI as compared to the NTP. About 31% and 37% saving in irrigation water was observed during Kharif and Rabi seasons, respectively in both methods of SRI cultivation over NTP. SRI performed well and consistently reduced requirement of inputs such as seed and water in different soil conditions. SRI method, using less water for rice production can help in overcoming water shortage in future and it can also make water available for growing other crops thus promoting crop diversificatio

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Assessment of yield gaps and income augmentation through participatory cluster front line demonstrations on Indian Mustard (Brassica juncea) in RajasthanThe study assesses the yield gaps and income augmentation of 15670 CFLDs on Mustard from 2015 to 2020 in 5417.45 hectares (ha) by Krishi Vigyan Kendras (KVKs) Rajasthan state. The researcher found the yield of CFLDs higher (18.59q/ha) than farmers' practices (14.70q/ha). The study depicted the technology gap of 7.54q/ha, the extension gap of 3.89q/ha, and a technology index of 28.26%. The CFLDs realized an additional yield of 3.89q/ha. Subsequently, Rs.15337.00/ha are supplemented, making a total of Rs.9.63 crores. The yields of CFLDs surpassed the state yield by 7.02q/ha and the national yield by 7.53q/ha. The identified technology gaps are attributed to dissimilarity in soil fertility status, weather conditions, and climate vulnerability. However, the adoption of integrated crop management practices could reduce the extension gaps in Mustard. Experts of KVKs should adopt location-specific extension methodologies for approaching partner farmers for better results. Moreover, the lower value of the technology index indicates greater technology feasibility in the particular district.Not Availabl

Combined analysis of remote sensing, gravity and magnetic data across Moyar Bhavani Shear Zone, Southern Granulite Terrane (SGT), India: appraisals for crustal architecture and tectonics

We present a combined analysis of remote sensing, gravity and magnetic data across Moyar Bhavani Shear Zone (MBSZ), Southern Granulite Terrane (SGT), India to study crustal architecture and tectonics. The horizontal gradient of gravity data shows strong density inhomogeneity across the MBSZ. The magnetic data and residual gravity analysis shows corroboration of a NE–SW trending linear magnetic anomaly zone and residual gravity high strip along the MBSZ. The low magnetic anomalies dominate the northern part of the MBSZ, while the southern part is dominated by moderate to high anomalies. The integration of potential data and remote sensing data clearly reveals that ENE–WSW is the dominant structural trend that coincides with the Mettur Shear Zone (MeSZ) trend. The joint gravity and magnetic modelling suggest that the MBSZ, is characterized by the disposition of mélange of moderate to high-density rocks of diamagnetic nature

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Sol-gel synthesized BiFeO3-Graphene nanocomposite as efficient electrode for supercapacitor application

In the present work, bismuth iron oxide-graphene composite has been explored as electrode material for electrochemical supercapacitor application. Bismuth iron oxide (BFO) nanoparticles, synthesized by sol-gel process, are mixed with the graphene sheets in a solution. The electrodes are prepared by coating the resulted slurry on stainless steel (SS) substrate, by drop casting process. The morphology and structure of the BFO-graphene composite are characterized by XRD, FIB-SEM, HRTEM and Raman spectroscopy, which show that the nanoparticles with diameter 100-200 nm are randomly distributed on and around the graphene sheets. The composite electrode exhibits significantly enhanced capacitance as compared to BFO. In this structure, the electrons generated by the surface based Faradaic reactions from the BFO nanoparticles can be transported by the graphene nanosheets toward the current collector. The electrochemical characteristic of the electrodes is investigated through cyclic voltammetry and charging/discharging process. The specific capacitance of the electrode measured at 5-100 mV/s was found to be 17-4 mF/cm(2) which is comparable to the most commonly used metal oxide based electrode materials. It shows better cycling stability with 95% retention of capacitance after 2000 cycles

Differential gene expression analysis in germinating and dormant teliospores of Tilletia indica using RNA seq approach

214-223Karnal bunt of wheat is an important quarantine disease that interrupts India’s wheat trade in the international market. The whole transcriptome of germinating and dormant teliospores of Tilletia indica was performed using the RNA Seq approach to identify germination-related genes. Approximately 63 million reads were generated using the RNA sequencing by the Illumina NextSeq500 platform. The high-quality reads were deposited in NCBI SRA database (accession: PRJNA522347). The unigenes from the pooled teliospores were 16,575 having unigenes length of 28,998,753 bases. The high-quality reads of germinating teliospores mapped on to 21,505 predicted CDSs. 9,680 CDSs were common between dormant and germinating teliospores of T. indica. 11,825 CDSs were found to be in germinating teliospores while only 91 were unique in dormant spores of pathogen. The pathway analysis showed the highest number of pathways was found in germinating spores than dormant spores. The highest numbers of CDSs were found to be associated with translation (431 in number), transport and catabolism (340), signal transduction (326), and carbohydrate metabolism (283). The differential expression analysis (DESeq) of germinating and dormant teliospores showed that 686 CDS were up-regulated and 114 CDS were down-regulated in the germinating teliospores. Significant germination-related genes in the spores were validated using qPCR analysis. Ten genes viz. Ti3931, Ti6828, Ti7098, Ti7462, Ti7522, Ti 9289, Ti 8670, Ti 7959, Ti 7809,and Ti10095 were highly up-regulated in germinated teliospores which may have role in germination of spores.Further, these differentially expressed genes provide insights into the molecular events. This first study of transcriptome will be helpful to devise better management strategies to manage Karnal bunt disease