47 research outputs found
Host genetics and pathogen species modulate infection-induced changes in social aggregation behaviour
Identifying how infection modifies host behaviours that determine social contact networks is important for understanding heterogeneity in infectious disease dynamics. Here, we investigate whether group social behaviour is modified during bacterial infection in fruit flies (Drosophila melanogaster) according to pathogen species, infectious dose, host genetic background and sex. In one experiment, we find that systemic infection with four different bacterial species results in a reduction in the mean pairwise distance within infected female flies, and that the extent of this change depends on pathogen species. However, susceptible flies did not show any evidence of avoidance in the presence of infected flies. In a separate experiment, we observed genetic- and sex-based variation in social aggregation within infected, same-sex groups, with infected female flies aggregating more closely than infected males. In general, our results confirm that bacterial infection induces changes in fruit fly behaviour across a range of pathogen species, but also highlight that these effects vary between fly genetic backgrounds and can be sex-specific. We discuss possible explanations for sex differences in social aggregation and their consequences for individual variation in pathogen transmission
Threshold values for the protection of marine ecosystems from NORM in subsea oil and gas infrastructure
Acknowledgements The authors thank Professor Claus Otto (Curtin University) and Professor Richard Neilson (National Decommissioning Centre, Aberdeen, UK.) for comments and support to the project team; and, Sam Jarvis (National Environment Resources Australia), Professor Peter Macreadie, Dr Rick Tinker, and the industry partners of the National Decommissioning Research Initiative for helpful comments to this project. Funding This research was funded by the Australian Governmentâs Industry Growth Centre National Energy Resources Australia (NERA) through a National Decommissioning Research Initiative (NDRI) grant to Curtin University. The NDRI project was funded by eight industry partners including Shell Australia, Esso Australia, Chevron Australia, BHP Petroleum, Woodside Energy, Santos Limited, ConocoPhillips Pipeline Australia, and Vermilion Oil and Gas Australia. AH is partly funded by the National Decommissioning Centre, Scotland, and the University of Aberdeen.Peer reviewedPublisher PD
Radiological risk assessment to marine biota from exposure to NORM from a decommissioned offshore oil and gas pipeline
Scale residues can accumulate on the interior surfaces of subsea petroleum pipes and may incorporate naturally occurring radioactive materials (NORM). The persistent nature of âNORM scaleâ may result in a radiological dose to the organisms living on or near intact pipelines. Following a scenario of in-situ decommissioning of a subsea pipeline, marine organisms occupying the exteriors or interiors of petroleum structures may have close contact with the scale or other NORM-associated contaminated substances and suffer subsequent radiological effects. This case study used radiological dose modelling software, including the ERICA Tool (v2.0), MicroShieldÂź Pro and mathematical equations, to estimate the likely radiological doses and risks of effects from NORM-contaminated scale to marine biota from a decommissioned offshore oil and gas pipeline. Using activity concentrations of NORM (226Ra, 210Po, 210Pb, 228Ra, 228Th) from a subsea pipeline from Australia, environmental realistic exposure scenarios including radiological exposures from both an intact pipe (external only; accounting for radiation shielding by a cylindrical carbon steel pipe) and a decommissioned pipeline with corrosive breakthrough (resulting in both internal and external radiological exposure) were simulated to estimate doses to model marine organisms. Predicted dose rates for both the external only exposure (ranging from 26 ÎŒGy/h to 33 ÎŒGy/h) and a corroded pipeline (ranging from 300 ÎŒGy/h to 16,000 ÎŒGy/h) exceeded screening levels for radiological doses to environmental receptors. The study highlighted the importance of using scale-specific solubility data (i.e., Kd) values for individual NORM radionuclides for ERICA assessments. This study provides an approach for conducting marine organism dose assessments for NORM-contaminated subsea pipelines and highlights scientific gaps required to undertake risk assessments necessary to inform infrastructure decommissioning planning
The ENIGMA Stroke Recovery Working Group: Big data neuroimaging to study brainâbehavior relationships after stroke
The goal of the Enhancing Neuroimaging Genetics through MetaâAnalysis (ENIGMA) Stroke Recovery working group is to understand brain and behavior relationships using wellâpowered metaâ and megaâanalytic approaches. ENIGMA Stroke Recovery has data from over 2,100 stroke patients collected across 39 research studies and 10 countries around the world, comprising the largest multisite retrospective stroke data collaboration to date. This article outlines the efforts taken by the ENIGMA Stroke Recovery working group to develop neuroinformatics protocols and methods to manage multisite stroke brain magnetic resonance imaging, behavioral and demographics data. Specifically, the processes for scalable data intake and preprocessing, multisite data harmonization, and largeâscale stroke lesion analysis are described, and challenges unique to this type of big data collaboration in stroke research are discussed. Finally, future directions and limitations, as well as recommendations for improved data harmonization through prospective data collection and data management, are provided
Chronic Stroke Sensorimotor Impairment Is Related to Smaller Hippocampal Volumes: An ENIGMA Analysis
Background.
Persistent sensorimotor impairments after stroke can negatively impact quality of life. The hippocampus is vulnerable to poststroke secondary degeneration and is involved in sensorimotor behavior but has not been widely studied within the context of poststroke upperâlimb sensorimotor impairment. We investigated associations between nonâlesioned hippocampal volume and upper limb sensorimotor impairment in people with chronic stroke, hypothesizing that smaller ipsilesional hippocampal volumes would be associated with greater sensorimotor impairment.
Methods and Results.
Crossâsectional T1âweighted magnetic resonance images of the brain were pooled from 357 participants with chronic stroke from 18 research cohorts of the ENIGMA (Enhancing NeuoImaging Genetics through MetaâAnalysis) Stroke Recovery Working Group. Sensorimotor impairment was estimated from the FMAâUE (FuglâMeyer Assessment of Upper Extremity). Robust mixedâeffects linear models were used to test associations between poststroke sensorimotor impairment and hippocampal volumes (ipsilesional and contralesional separately; Bonferroniâcorrected, P<0.025), controlling for age, sex, lesion volume, and lesioned hemisphere. In exploratory analyses, we tested for a sensorimotor impairment and sex interaction and relationships between lesion volume, sensorimotor damage, and hippocampal volume. Greater sensorimotor impairment was significantly associated with ipsilesional (P=0.005; ÎČ=0.16) but not contralesional (P=0.96; ÎČ=0.003) hippocampal volume, independent of lesion volume and other covariates (P=0.001; ÎČ=0.26). Women showed progressively worsening sensorimotor impairment with smaller ipsilesional (P=0.008; ÎČ=â0.26) and contralesional (P=0.006; ÎČ=â0.27) hippocampal volumes compared with men. Hippocampal volume was associated with lesion size (P<0.001; ÎČ=â0.21) and extent of sensorimotor damage (P=0.003; ÎČ=â0.15).
Conclusions.
The present study identifies novel associations between chronic poststroke sensorimotor impairment and ipsilesional hippocampal volume that are not caused by lesion size and may be stronger in women.S.-L.L. is supported by NIH K01 HD091283; NIH R01 NS115845. A.B. and M.S.K. are supported by National Health and Medical Research Council (NHMRC) GNT1020526, GNT1045617 (A.B.), GNT1094974, and Heart Foundation Future Leader Fellowship 100784 (A.B.). P.M.T. is supported by NIH U54 EB020403. L.A.B. is supported by the Canadian Institutes of Health Research (CIHR). C.M.B. is supported by NIH R21 HD067906. W.D.B. is supported by the Heath Research Council of New Zealand. J.M.C. is supported by NIH R00HD091375. A.B.C. is supported by NIH R01NS076348-01, Hospital Israelita Albert Einstein 2250-14, CNPq/305568/2016-7. A.N.D. is supported by funding provided by the Texas Legislature to the Lone Star Stroke Clinical Trial Network. Its contents are solely the responsibility of the authors and do not necessarily represent the of ficial views of the Government of the United States or the State of Texas. N.E.-B. is supported by Australian Research Council NIH DE180100893. W.F. is sup ported by NIH P20 GM109040. F.G. is supported by Wellcome Trust (093957). B.H. is funded by and NHMRC fellowship (1125054). S.A.K is supported by NIH P20 HD109040. F.B. is supported by Italian Ministry of Health, RC 20, 21. N.S. is supported by NIH R21NS120274. N.J.S. is supported by NIH/National Institute of General Medical Sciences (NIGMS) 2P20GM109040-06, U54-GM104941. S.R.S. is supported by European Research Council (ERC) (NGBMI, 759370). G.S. is supported by Italian Ministry of Health RC 18-19-20-21A. M.T. is sup ported by National Institute of Neurological Disorders and Stroke (NINDS) R01 NS110696. G.T.T. is supported by Temple University sub-award of NIH R24 âNHLBI (Dr Mickey Selzer) Center for Experimental Neurorehabilitation Training. N.J.S. is funded by NIH/National Institute of Child Health and Human Development (NICHD) 1R01HD094731-01A1
A large, curated, open-source stroke neuroimaging dataset to improve lesion segmentation algorithms.
Accurate lesion segmentation is critical in stroke rehabilitation research for the quantification of lesion burden and accurate image processing. Current automated lesion segmentation methods for T1-weighted (T1w) MRIs, commonly used in stroke research, lack accuracy and reliability. Manual segmentation remains the gold standard, but it is time-consuming, subjective, and requires neuroanatomical expertise. We previously released an open-source dataset of stroke T1w MRIs and manually-segmented lesion masks (ATLAS v1.2, Nâ=â304) to encourage the development of better algorithms. However, many methods developed with ATLAS v1.2 report low accuracy, are not publicly accessible or are improperly validated, limiting their utility to the field. Here we present ATLAS v2.0 (Nâ=â1271), a larger dataset of T1w MRIs and manually segmented lesion masks that includes training (nâ=â655), test (hidden masks, nâ=â300), and generalizability (hidden MRIs and masks, nâ=â316) datasets. Algorithm development using this larger sample should lead to more robust solutions; the hidden datasets allow for unbiased performance evaluation via segmentation challenges. We anticipate that ATLAS v2.0 will lead to improved algorithms, facilitating large-scale stroke research
Association of Brain Age, Lesion Volume, and Functional Outcome in Patients With Stroke
BACKGROUND AND OBJECTIVES: Functional outcomes after stroke are strongly related to focal injury measures. However, the role of global brain health is less clear. In this study, we examined the impact of brain age, a measure of neurobiological aging derived from whole-brain structural neuroimaging, on poststroke outcomes, with a focus on sensorimotor performance. We hypothesized that more lesion damage would result in older brain age, which would in turn be associated with poorer outcomes. Related, we expected that brain age would mediate the relationship between lesion damage and outcomes. Finally, we hypothesized that structural brain resilience, which we define in the context of stroke as younger brain age given matched lesion damage, would differentiate people with good vs poor outcomes.
METHODS: We conducted a cross-sectional observational study using a multisite dataset of 3-dimensional brain structural MRIs and clinical measures from the ENIGMA Stroke Recovery. Brain age was calculated from 77 neuroanatomical features using a ridge regression model trained and validated on 4,314 healthy controls. We performed a 3-step mediation analysis with robust mixed-effects linear regression models to examine relationships between brain age, lesion damage, and stroke outcomes. We used propensity score matching and logistic regression to examine whether brain resilience predicts good vs poor outcomes in patients with matched lesion damage.
RESULTS: We examined 963 patients across 38 cohorts. Greater lesion damage was associated with older brain age (ÎČ = 0.21; 95% CI 0.04-0.38,
DISCUSSION: We provide evidence that younger brain age is associated with superior poststroke outcomes and modifies the impact of focal damage. The inclusion of imaging-based assessments of brain age and brain resilience may improve the prediction of poststroke outcomes compared with focal injury measures alone, opening new possibilities for potential therapeutic targets
Ecotoxicological effects of decommissioning offshore petroleum infrastructure: a systematic review
Mode of access: World Wide WebTheoretical thesis.Bibliography: pages 48-66.1. Introduction -- 2. Methodology -- 3. Results and discussion -- 4. Research gaps -- 5. Future directions -- References -- Supplementary material.Successful decommissioning of subsea oil and gas infrastructure requires a safe and effective approach to assess and manage waste products. These products, often present as scale on internals of pipelines, include naturally occurring radioactive materials (NORM) and trace metals. Understanding the potential biological effects of these contaminants on marine fauna is crucial to managing global decommissioning. A systematic review was conducted, and information extracted from available literature on the biological effects of contaminants on marine organisms and current environmental regulations of petroleum-associated NORM management. Studies defining the chemical and radiological effects from decommissioned structures were limited. The main source of contaminants was identified from offshore platforms, with none from subsea structures. Only three studies measured variable chemical effects of radium to organisms from scale materials in subsea oil and gas infrastructure. Current international regulations are absent for the closure of subsea pipelines with NORM being underreported and not addressed in environmental impact assessments. This review highlights research gaps from environmental monitoring and characterisation of NORM associated with decommissioned structures. Key recommendations for future research include using available techniques to monitor and characterise NORM scale and assess effects of scale to marine organisms through direct organism exposure experiments. This study provides guidance for the formation of appropriate risk assessments and decommissioning decisions incorporating ecotoxicological principles.Mode of access: Internet.1 online resource (75 pages) diagrams, graphs, table
An Empirical Evaluation of Environmental Citizen Suits under the Environmental Protection and Biodiversity Conservation Act 1999 (Cth)
Since the early 1970s, many jurisdictions have broadened legal standing rules to encourage environmental citizen suits and improve compliance with public rights. Although now widely adopted, expanded standing provisions are frequently criticised on the grounds they can give rise to significant social costs. This article sheds light on the costs associated with expanded standing provisions by evaluating the impacts of citizen suits taken under the Australian Governmentâs principal environmental statute, the Environment Protection and Biodiversity Conservation Act 1999 (Cth). The results suggest the social costs of citizen suit activity under the Act over the period 2000â2015 were negligible. Environmental citizen suits were seldom taken and rarely won; the substantive effects of successful suits were often nullified by subsequent executive action; and the suits generally had negligible or minor effects on project timelines
Outcomes from 10 years of biodiversity offsetting
We quantified net changes to the area and quality of native vegetation after the introduction of biodiversity offsetting in New South Wales, Australiaâa policy intended to âprevent broadâscale clearing of native vegetation unless it improves or maintains environmental values.â Over 10 years, a total of 21,928 ha of native vegetation was approved for clearing under this policy and 83,459 ha was established as biodiversity offsets. We estimated that no net loss in the area of native vegetation under this policy will not occur for 146 years. This is because 82% of the total area offset was obtained by averting losses to existing native vegetation and the rate that these averted losses accrue was overâestimated in the policy. There were predicted net gains in 10 of the 14 attributes used to assess the quality of habitat. An overall net gain in the quality of habitat was assessed under this policy by substituting habitat attributes that are difficult to restore (e.g. mature trees) with habitat attributes for which restoration is relatively easy (e.g. tree seedlings). Longâterm rates of annual deforestation did not significantly change across the study area after biodiversity offsetting was introduced. Overall, the policy examined here provides no net loss of biodiversity: (i) many generations into the future, which is not consistent with intergenerational equity; and (ii) by substituting different habitat attributes, so gains are not equivalent to losses. We recommend a number of changes to biodiversity offsetting policy to overcome these issues