Angiogenin mRNA Expression Levels in Prostate Cancer Tissue

Introduction: Prostate cancer is the most commonly diagnosed cancer in men and second leading cause of cancer deaths. Studies have shown that tRNA fragments are upregulated in prostate cancers and play important roles in carcinogenesis. This project looks at how tRNA cleaving enzyme angiogenin expression is regulated in prostate cancer tissues. Methods: Clinical data and mRNA expression levels of selected tRNA cleaving enzymes were extracted from the TCGA website. mRNAs were sequenced using IlluminaGA_RNASeqV2 at University of North Carolina. Results: 546 samples from 494 patients, with normal tissue from 53 patients were collected. ANG mRNA levels were lower in patients with higher Gleason scores(Intercept=1321.787362, regression coefficient= -87.05499452, R2=0.038). ANG mRNA levels were inconclusive in different clinical T grade(p=0.15), but were lower in higher pathologic T grade(intercept=1100.484695, x variable=-166.9047227, R2=0.038); ANG expression was lower in patients with nodal involvement versus without(539.56 vs 673.58, p=0.005). Discussion: Overall trend we found from the results were ANG mRNA expression levels are down regulated in patients that have more advanced disease versus early disease. This supports the hypothesis that ANG expression plays an interesting role in prostate cancer biology. This trend might be due to the negative feedback due to high levels of tRNA fragments however there is no single theory available to answer this question

Determinants of Health-Promoting Lifestyle among Nurses in Taiwan

This two-phase study was undertaken to: (1) determine the relationships of self-efficacy, perceived health status, perceived social support, age, marital status, education, work shift, work setting, and years employed as a registered nurse (RN) to the practice of a health-promoting lifestyle; (2) determine the combination of predictor variables explaining the variance in the practice of a health-promoting lifestyle; and (3) investigate other personal and environmental cues and characteristics related to health-promoting lifestyles among nurses (N = 218) in Taiwan. Findings of the quantitative approach in Phase One indicated that a health-promoting lifestyle was significantly related to self-efficacy, perceived health status, perceived social support, age, and years employed as an RN. Four predictor variables, namely, self-efficacy, perceived health status, perceived social support, and working the evening shift, explained 40.4% of the variance in health-promoting lifestyle in this sample. Responses to open-ended questions revealed other factors that contribute to health-promoting lifestyle among the nurses. In Phase Two, nine subjects who scored very high and 10 who scored very low on the Health-Promoting Lifestyle Profile (HPLP) were interviewed regarding health beliefs, behaviors and factors influencing the practice of a health-promoting lifestyle. The interview data partly validate the findings from Phase One. In many situations, the subjects in Phase Two of this study cited predictor variables investigated such as social supports and rotating shift. Subjects also stressed the importance of energy, perseverance and partners in initiating and maintaining a health-promoting lifestyle. The interview data revealed other personal and environmental cues and characteristics of a health-promoting lifestyle. The high group had initiated less lifestyle changes, but maintained the changes longer than subjects in the low group. They also identified more enabling characteristics than did the low group. Subjects in the low group used more stress management techniques and identified more hindrances for lifestyle changes than the high HPLP group. A revised model was developed for testing in future studies

A four gene signature of chromosome instability (CIN4) predicts for benefit from taxanes in the NCIC-CTG MA21 clinical trial.

Recent evidence demonstrated CIN4 as a predictive marker of anthracycline benefit in early breast cancer. An analysis of the NCIC CTG MA.21 clinical trial was performed to test the role of existing CIN gene expression signatures as prognostic and predictive markers in the context of taxane based chemotherapy.RNA was extracted from patients in cyclophosphamide, epirubicin and flurouracil (CEF) and epirubicin, cyclophosphamide and paclitaxel (EC/T) arms of the NCIC CTG MA.21 trial and analysed using NanoString technology.After multivariate analysis both high CIN25 and CIN70 score was significantly associated with an increased in RFS (HR 1.76, 95%CI 1.07-2.86, p=0.0018 and HR 1.59, 95%CI 1.12-2.25, p=0.0096 respectively). Patients whose tumours had low CIN4 gene expression scores were associated with an increase in RFS (HR: 0.64, 95% CI 0.39-1.03, p=0.06) when treated with EC/T compared to patients treated with CEF.In conclusion we have demonstrated CIN25 and CIN70 as prognostic markers in breast cancer and that CIN4 is a potential predictive maker of benefit from taxane treatment

Évaluation de la potabilité chimique des eaux souterraines dans un bassin versant tropical :Cas du Sud-Ouest de la Côte d’Ivoire

L'etude propose un etat des lieux de la qualite chimique des eaux souterraines ainsi que leur situation vis-a-vis d'elements toxiques d'origine anthropique (nitrate et pesticides) dans la nouvelle boucle du cacao (Sud-Ouest de la Cote dfIvoire). Cinquante-six echantillons dfeau souterraine et de surface ont ete preleves au cours de deux campagnes de terrain puis analyses en se basant sur seize parametres physico-chimiques. Les resultats montrent que les eaux souterraines ont pour la majorite un pH neutre. Elles sont a 34% tres faiblement mineralisees ; seulement 21% presente une conductivite superieure a 500 ƒÊS.cm-1. Ces eaux sont pour la plupart bicarbonatees dont une moitie est alcaline et lfautre calco-magnesienne. La qualite chimique est satisfaisante pour 80% des eaux etudiees au regard des normes OMS. Certaines ont meme des teneurs voisines a certaines eaux minerales de commerce. En revanche, dfautres presentent des teneurs tres critiques en nitrate, en sulfate et en fer. Les fortes teneurs en nitrate proviennent des activites humaines demontrant que les ressources en eau de la region ne sont pas a lfabri dfune pollution par les pesticides qui sont fortement utilises dans les plantations de cacao, cafe, hevea et palmier a huile ainsi que dans les maraichers.Mots cles: Qualite physico-chimique, pesticides, nitrate, hydrochimie, Cote dfIvoire

Childhood cancer in Côte d’Ivoire, 1995 - 2004 – challenges and hopes

Background. There is insufficient research into the state of paediatric oncology in African countries.Objectives. The purpose of this study was to analyse the state of paediatric oncology between 1995 and 2004 in Côte d’Ivoire.Methods. This retrospective descriptive study analysed all patients under the age of 18 who were diagnosed with cancer in Côte d’Ivoireover a period of 10 years (January 1995 - December 2004) with regard to demographics, types of pathology, delay in diagnosis andtreatment, treatment modalities, abandonment of treatment and survival rate.Results. Of 405 patients diagnosed with cancer, 331 were included in the study. Burkitt’s lymphoma was the most common malignancy (73.6%), followed by nephroblastoma (14.5%) and acute leukaemia (4%). Delay in diagnosis occurred in 38.7% of cases and ranged from 1 to 3 months; the average delay from diagnosis to starting treatment was 18 days. An abdominal mass and swelling of the jaw were the most common clinical presentations. Almost half of the patients (48.6%) were lost to follow-up and over a third (39.3%) died shortly after admission owing to advanced disease. The overall survival rate was 9.4%.Conclusions. Cancer in children in Côte d’Ivoire was dominated by Burkitt’s lymphoma. The rate of loss to follow-up of almost 50% is grounds for concern. The overall survival rate of 9.4% is very low, but such figures are not uncommon for African countries. Collaboration within the Franco-African Group of Paediatric Oncology has contributed to improving the management of children with cancer

Lipidomics Reveals Early Metabolic Changes in Subjects with Schizophrenia: Effects of Atypical Antipsychotics

There is a critical need for mapping early metabolic changes in schizophrenia to capture failures in regulation of biochemical pathways and networks. This information could provide valuable insights about disease mechanisms, trajectory of disease progression, and diagnostic biomarkers. We used a lipidomics platform to measure individual lipid species in 20 drug-naïve patients with a first episode of schizophrenia (FE group), 20 patients with chronic schizophrenia that had not adhered to prescribed medications (RE group), and 29 race-matched control subjects without schizophrenia. Lipid metabolic profiles were evaluated and compared between study groups and within groups before and after treatment with atypical antipsychotics, risperidone and aripiprazole. Finally, we mapped lipid profiles to n3 and n6 fatty acid synthesis pathways to elucidate which enzymes might be affected by disease and treatment. Compared to controls, the FE group showed significant down-regulation of several n3 polyunsaturated fatty acids (PUFAs), including 20:5n3, 22:5n3, and 22:6n3 within the phosphatidylcholine and phosphatidylethanolamine lipid classes. Differences between FE and controls were only observed in the n3 class PUFAs; no differences where noted in n6 class PUFAs. The RE group was not significantly different from controls, although some compositional differences within PUFAs were noted. Drug treatment was able to correct the aberrant PUFA levels noted in FE patients, but changes in re patients were not corrective. Treatment caused increases in both n3 and n6 class lipids. These results supported the hypothesis that phospholipid n3 fatty acid deficits are present early in the course of schizophrenia and tend not to persist throughout its course. These changes in lipid metabolism could indicate a metabolic vulnerability in patients with schizophrenia that occurs early in development of the disease. © 2013 McEvoy et al

MAXIMIZING TECHNOLOGY ACCEPTANCE MODEL IN ACCESSING THE ATTITUDE OF RURAL FARMERS USING ICT TOOLS IN FARMING TO ENHANCE PRODUCTIVITY

The study was conducted using a multi-stage sampling technique involving simple random sampling, a probability sampling method, purposive sampling, and snowball sampling, which are non-probability methods. Two regions, namely, the Greater Accra and Eastern were purposively selected for the study. In the Greater Accra Region, Ada East District was selected while in the Eastern Region Asuogyaman District. In Greater Accra, the study was conducted in six farming villages namely: Big Ada, Dogo, Kasseh, Addokope, Korlekope, and Bedeku. In Eastern regions, the study was conducted in Asogyaman, where Tortibo, Sappor, Yenease, Adina Donor, and Ankyease. A snowball strategy was relied on in selecting small-scale farmers for the study. A total of 390 households, 130 from each district, were sampled randomly. The methodologies used for primary data were household surveys and Focus Group Discussion (FGD) while the instruments used were semi-structured pre-tested Interview Schedules and Checklist respectively. The study concludes that age has an effect on the attitude of small-scale farmers concerning their perceived use of ICT to improve their farming activities. The educational level of an individual plays a significant role in the acceptance and use of ICT

Synovial chondrosarcoma arising from synovial chondromatosis of the knee

We report the case of a 51-year-old woman who had suffered from right knee pain and stiffness for 40 years. Her symptoms had gradually worsened over the past 5 years. One year previously, when she first visited our clinic, plain radiographs and CT scan images had revealed synovial chondromatosis over the right knee. At the current admission, follow-up MRI showed synovial masses around the knee and worsening of endosteal cortical scalloping in the patella, femoral condyle, and tibial plateau. After diagnosis on the basis of frozen sections, the patient had total excision of the lesions and total knee athroplasty. Histological examination revealed synovial chondromatosis in the joint cavity and grade 1 chondrosarcoma invasion into the adjacent bone. In long-standing synovial chondromatosis, presentation with aggravated symptoms and deterioration on imaging findings should alert clinicians to the potential for malignant change

A genetic contribution from the Far East into Ashkenazi Jews via the ancient Silk Road

Contemporary Jews retain a genetic imprint from their Near Eastern ancestry, but obtained substantial genetic components from their neighboring populations during their history. Whether they received any genetic contribution from the Far East remains unknown, but frequent communication with the Chinese has been observed since the Silk Road period. To address this issue, mitochondrial DNA (mtDNA) variation from 55,595 Eurasians are analyzed. The existence of some eastern Eurasian haplotypes in eastern Ashkenazi Jews supports an East Asian genetic contribution, likely from Chinese. Further evidence indicates that this connection can be attributed to a gene flow event that occurred less than 1.4 kilo-years ago (kya), which falls within the time frame of the Silk Road scenario and fits well with historical records and archaeological discoveries. This observed genetic contribution from Chinese to Ashkenazi Jews demonstrates that the historical exchange between Ashkenazim and the Far East was not confined to the cultural sphere but also extended to an exchange of genes