4 research outputs found
Endocarditis By Kocuria Rosea In An Immunocompetent Child
Kocuria rosea belongs to genus Kocuria (Micrococcaceae family, suborder Micrococcineae, order Actinomycetales) that includes about 11 species of bacteria. Usually, Kocuria sp are commensal organisms that colonize oropharynx, skin and mucous membrane Kocuria sp infections have been described in the last decade commonly affecting immunocompromised patients, using intravenous catheter or peritoneal dialysis. These patients had mainly bacteremia/recurrent sepsis. We hereby describe the case of a 10-year-old girl, immunocompetent, who had endocarditis/sepsis by K. rosea which was identified in five different blood cultures by Vitek 2 ID-GPC card (BioMérieux, France). Negative HIV serology, blood count within normal range of leukocytes/neutrophils and lymphocytes, normal fractions of the complement, normal level of immunoglobulins for the age lymphocyte immunophenotyping was also within the expected values. Thymus image was normal at chest MRI. No catheters were required. Identification of K. rosea was essential to this case, allowing the differentiation of coagulase-negative staphylococci and use of an effective antibiotic treatment. Careful laboratory analysis of Gram-positive blood-born infections may reveal more cases of Kocuria sp infections in immunocompetent patients, which may collaborate for a better understanding, prevention and early treatment of these infections in pediatrics.1918284Savini, V., Catavitello, C., Masciarelli, G., Drug sensitivity and clinical impact of members of the genus Kocuria (2010) J Med Microbiol, 59, pp. 1395-1402Becker, K., Rutsch, F., Uekötter, A., Kocuria rhizophila adds to the emerging spectrum of micrococcal species involved in human infections (2008) J Clin Microbiol, 46, pp. 3537-3539Dotis, J., Printza, N., Papachristou, F., Peritonitis attributable to Kocuria rosea in a pediatric peritoneal dialysis patient (2012) Perit Dial Int, 32, pp. 577-578Moissenet, D., Becker, K., Mérens, A., Persistent bloodstream infection with Kocuria rhizophila related to a damaged central catheter (2012) J Clin Microbiol, 50, pp. 1495-1498Chen, H.M., Chi, H., Chiu, N.C., Kocuria kristinae: a true pathogen in pediatric patients (2013) J Microbiol Immunol InfectKaradag Oncel, E., Boyraz, M.S., Kara, A., Black tongue associated with Kocuria (Micrococcus) kristinae bacteremia in a 4-month-old infant (2012) Eur J Pediatr, 171, p. 593Lai, C.C., Wang, J.Y., Lin, S.H., Catheter-related bacteraemia and infective endocarditis caused by Kocuria species (2011) Clin Microbiol Infect, 17, pp. 190-192Rushani, D., Kaufman, J.S., Ionescu-Ittu, R., Infective endocarditis in children with congenital heart disease: cumulative incidence and predictors (2013) Circulation, 128, pp. 1412-1419Srinivasa, K.H., Agrawal, N., Agarwal, A., Dancing vegetations: Kocuria rosea endocarditis (2013) BMJ Case Rep, 28Kumar, C.G., Sujitha, P., Kocuran an exopolysaccharide isolated from Kocuria rosea strain BS-1 and evaluation of its in vitro immunosuppression activities (2014) Enzyme Microb Technol, 55, pp. 113-120Ben-Ami, R., Navon-Venezia, S., Schwartz, D., Erroneous reporting of coagulase-negative staphylococci as Kocuria spp. by the Vitek 2 system (2005) J Clin Microbiol, 43, pp. 1448-1450Boudewijns, M., Vandeven, J., Verhaegen, J., Vitek 2 automated identification system and Kocuria kristinae (2005) J Clin Microbiol, 43, p. 583
Performance of carassius auratus with different food strategies in water recirculation system
The efficiency of a recirculation system for the
cultivation of C. auratus, fed with natural and
artificial diets was evaluated. The experiment
consisted of four treatments with four replicates.
The first two treatments used recirculation system
and the last two static system. In the first and
fourth treatment, animals were fed with brine
shrimp biomass (50% CP), while in the second and
third treatments a commercial flake ration (42%
CP) was used. At the end of cultivation (60 days),
water samples were taken from all experimental
units and subjected to standard plate count for
determining the density of mesophilic bacteria.
Final weight (g), final length (cm), specific growth
rate (% day -1 ) and weight gain (%) were significantly
different (p<0.05) among tested diets, but the
culture systems did not affect fish performance.
There was significant difference (p<0.05)
between systems for total ammonia and nitrite.
Animals fed with brine shrimp biomass acquired
more intense and brighter colors than those fed
only with commercial flake food. The values of
standard plate count of mesophilic bacteria ranged
from 7.0 × 10 3 to 1.1 × 10 4 CFU.ml -1 . In this study
we observed that C. auratus is a species tolerant
to adverse water conditions, perhaps with
increased stock densities, the recirculation system
could play some positive role on the zootechnical
performance of cultured animals.A eficiência de um sistema de recirculação
para o cultivo de C. auratus, alimentados com dieta
natural e artificial foi avaliada. O experimento
consistiu de quatro tratamentos com quatro
repetições. Os dois primeiros tratamentos
utilizaram sistemas de recirculação e os dois
últimos sistemas estáticos. No primeiro e quarto
tratamento, os animais foram alimentados com
biomassa de artêmia (PB 50%), enquanto nos
tratamentos dois e três, com ração comercial em
flocos (42% PB). Ao final do cultivo (60 dias),
amostras de água foram coletadas em todas as
unidades experimentais e submetidos a contagem
padrão em placas para determinação da densidade
de bactérias mesófilas. O peso final (g), com-
primento final (cm), taxa de crescimento especí-
fico (% dia -1 ) e ganho de peso (%) foram signifi-
cativamente diferentes (p<0,05) entre as dietas
testadas, mas os sistemas de cultivo não afetaram
o desempenho dos peixes. Houve diferença sig-
nificativa (p<0,05) entre os sistemas para os
níveis de amônia total e nitrito. Os animais alimen-
tados com biomassa artêmia adquiriram cor e
brilho mais intensos do que aqueles alimentados
apenas com ração comercial em flocos. Os valo-
res de contagem padrão em placas de bactérias
mesófilas variaram de 7,0 × 10 3 para 1,1 × 10 4
CFU.ml -1 . Neste estudo, observou-se que C.
auratus é uma espécie tolerante a condições
adversas de água, talvez com maior densidade de
estocagem, o sistema de recirculação poderia
desempenhar papel positivo sobre o desempenho
zootécnico dos animais cultivados